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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/04/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SÁ, J. F. de; SAMPAIO, E. dos S.; MENDES, M. I. de S.; SANTOS, K. C. F. dos; SOUZA, A. da S.; LEDO, C. A. da S. |
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Afiliação: |
JUCIENY FERREIRA DE SÁ, UFRB; UFRB; MARIA INÊS DE SOUZA MENDES, UESC; KAREN CRISTINA FIALHO DOS SANTOS, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Culture media for the multiplication of wild Manihot species meios de cultura para a multiplicação de espécies silvestres de Manihot. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v.42, n.6, p.598-607, Nov./Dec. 2018 |
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ISSN: |
1981-1829 |
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Idioma: |
Inglês |
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Conteúdo: |
The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species belonging to genus Manihot. MenosThe cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species... Mostrar Tudo |
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Thesagro: |
Cultura de Tecido; Mandioca; Micropropagação. |
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Thesaurus Nal: |
Micropropagation; Tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | OKINO, C. H.; MONTASSIER, M. de F. S.; SILVA, K. R.; FERNANDO, F. S.; BRENTANO, L.; MONTASSIER, H. J. Avian infectious bronchitis vírus (IBV): effect of vaccine doses on mucosal imune responses and protection after challenge in chickens. In: WORLD´S POULTRY CONGRESS, 24., 2012, Salvador. Abstract... Salvador: WSPA, 2012. 1 CD-ROM. World's Poultry Science Journal, v. 68, supl. 1, 2012. Projeto/Plano de Ação: 05.11.11.002.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 2. | | SANTOS, I. L.; OKINO, C. H.; MONTASSIER, M. de F. S.; SILVA, K. R.; FERNANDO, F. S.; MONTASSIER, H. J. Immune-protection mechanisms against late challenge with avian infectious bronchitis vírus (IBV) induced by a single-dose of an attenuated vaccine in day-old chicks. In: WORLD´S POULTRY CONGRESS, 24., 2012, Salvador. Abstract... Salvador: WSPA, 2012. 1 CD-ROM. World's Poultry Science Journal, v. 68, supl. 1, 2012. Projeto/Plano de Ação: 05.11.11.002| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 3. | | SANTOS, I. L.; GIBERTONI, A. M.; MONTASSIER, M. de F. S.; OKINO, C. H.; MONTASSIER, H. J. Improvement of immunodiagnosis of avian infectious bronchitis virus (IBV) infection by the detection of IgM and IgG antibodies using a new recombinant nucleoprotein-based Elisa. In: WORLD´S POULTRY CONGRESS, 24., 2012, Salvador. Abstract... Salvador: WSPA, 2012. 1 CD-ROM. World's Poultry Science Journal, v. 68, supl. 1, 2012. Projeto/Plano de Ação: 05.11.11.002.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | OKINO, C. H.; ALESSI, A. C.; MONTASSIER, M. de F. S.; ROSA, A. J. de M.; WANG, X.; MONTASSIER, H. J. Humoral and Cell-Mediated Immune Responses to Different Doses of Attenuated Vaccine Against Avian Infectious Bronchitis Virus. Viral Immunology, Nova York, v. 26, n. 4, p. 1-9, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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| 6. | | LOPES, P. D.; OKINO, C. H.; FERNANDO, F. S.; PAVANI, C.; MARIGUELA, V. C.; MONTASSIER, M. DE F. S.; MONTASSIER, H. J. Comparative evaluation of immune responses and protection of chitosan nanoparticles and oil-emulsion adjuvants in avian coronavirus inactivated vaccines in chickens. Vaccines, v.9, n.12, 1457, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | SANTOS, R. M. dos; FERNANDO, F. S.; MONTASSIER, M. de F. S.; SILVA, K. R.; LOEPS, P. D.; PAVANI, C.; BORZI, M. M.; OKINO, C. H.; MONTASSIER, H. J. Memory immune responses and protection of chickens against a nephropathogenic infectious bronchitis virus strain by combining live heterologous and inactivated homologous vaccines. The Journal of Veterinary Medical Science, v.81, n.4, p.612-619, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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| Registros recuperados : 7 | |
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