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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/04/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SÁ, J. F. de; SAMPAIO, E. dos S.; MENDES, M. I. de S.; SANTOS, K. C. F. dos; SOUZA, A. da S.; LEDO, C. A. da S. |
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Afiliação: |
JUCIENY FERREIRA DE SÁ, UFRB; UFRB; MARIA INÊS DE SOUZA MENDES, UESC; KAREN CRISTINA FIALHO DOS SANTOS, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Culture media for the multiplication of wild Manihot species meios de cultura para a multiplicação de espécies silvestres de Manihot. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v.42, n.6, p.598-607, Nov./Dec. 2018 |
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ISSN: |
1981-1829 |
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Idioma: |
Inglês |
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Conteúdo: |
The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species belonging to genus Manihot. MenosThe cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species... Mostrar Tudo |
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Thesagro: |
Cultura de Tecido; Mandioca; Micropropagação. |
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Thesaurus Nal: |
Micropropagation; Tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 2. |  | BOLDRIN, P. F.; REIS, A. C. P. dos; BENITES, V. de M.; TAVARES, R. L. M.; MENEZES, J. F. S.; CANTÃO, V. C. G. Soil phosphorus and corn development under application of phosphate sources. Journal of Agricultural Science, v. 13, n. 9, p. 61-72, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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| 3. | | MENEZES, J. F. S.; TEODORO, A. de O. A.; BERTI, M. P. da S.; MENEZES, C. C. E. de; CANTÃO, V. C. G.; CAETANO, J. O.; BENITES, V. de M. Long-term application of swine manure can increase Cu and Zn contents in the pasture soils. Australian Journal of Crop Science, v. 11, n. 6, p. 744-748, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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