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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
12/12/2018 |
Data da última atualização: |
24/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. |
Afiliação: |
S. WOHLRES-VIANA, UFJF; E. K. N. ARASHIRO, UFF; T. P. MINARE, Universidade José do Rosario Vellano, Alfenas, MG; C. A. C. FERNANDES, Universidade Jose do Rosario Vellano / Biotran Biotecnologia Animal, Alfenas, MG; J. G. V. GRAZIA, UFMG; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
Título: |
Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Theriogenology, v. 126, p. 68-74, 2019. |
DOI: |
10.1016/j.theriogenology.2018.12.004 |
Idioma: |
Inglês |
Conteúdo: |
Abstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype. MenosAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0... Mostrar Tudo |
Palavras-Chave: |
In vivo embryo production. |
Thesaurus Nal: |
Embryo transfer; Luteinizing hormone. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02857naa a2200253 a 4500 001 2106166 005 2023-01-24 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1016/j.theriogenology.2018.12.004$2DOI 100 1 $aWOHLRES-VIANA, S. 245 $aDifferential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle.$h[electronic resource] 260 $c2019 520 $aAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype. 650 $aEmbryo transfer 650 $aLuteinizing hormone 653 $aIn vivo embryo production 700 1 $aARASHIRO, E. K. N. 700 1 $aMINARE, T. P. 700 1 $aFERNANDES, C. A. C. 700 1 $aGRAZIA, J. G. V. 700 1 $aSIQUEIRA, L. G. B. 700 1 $aMACHADO, M. A. 700 1 $aVIANA, J. H. M. 773 $tTheriogenology$gv. 126, p. 68-74, 2019.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
08/12/2014 |
Data da última atualização: |
09/12/2014 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, E. da; ROSA, M. G. da; SCHUHLI, G.; JAMES, S. W.; BARTZ, M. L. C.; NADOLNY, H.; BUSCH, E.; BROWN, G. G. |
Afiliação: |
Elodie da Silva, Pós-doutoranda da Embrapa Florestas; Marcio Gonçalves da Rosa, Universidade do Estado de Santa Catarina; GUILHERME SCHNELL E SCHUHLI, CNPF; Samuel Wooster James, University of Iowa; Marie Luise Carolina Bartz, Universidade Positivo; Herlon Nadolny, Doutorando da UFPR; Eliza Busch, UFPR; GEORGE GARDNER BROWN, CNPF. |
Título: |
O potencial do DNA barcode para a taxonomia de minhocas: identificação e conservação de espécies brasileiras. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 31.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 15.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 13.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 10., 2014, Araxá. Fertilidade e biologia do solo: integração e tecnologias para todos: anais. Araxá: Núcleo Regional Leste da Sociedade Brasileira de Ciência do Solo, 2014. |
Idioma: |
Português |
Notas: |
Resumo. FERTBIO 2014. |
Palavras-Chave: |
Brasil; Identificação molecular; Impedimento taxonômico; Oligoqueta. |
Thesagro: |
Biodiversidade; Minhoca. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/113242/1/2014-GuilhermeS-FERTBIO-OPotencial.pdf
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Marc: |
LEADER 01140nam a2200265 a 4500 001 2001883 005 2014-12-09 008 2014 bl uuuu u00u1 u #d 100 1 $aSILVA, E. da 245 $aO potencial do DNA barcode para a taxonomia de minhocas$bidentificação e conservação de espécies brasileiras.$h[electronic resource] 260 $aIn: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 31.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 15.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 13.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 10., 2014, Araxá. Fertilidade e biologia do solo: integração e tecnologias para todos: anais. Araxá: Núcleo Regional Leste da Sociedade Brasileira de Ciência do Solo$c2014 500 $aResumo. FERTBIO 2014. 650 $aBiodiversidade 650 $aMinhoca 653 $aBrasil 653 $aIdentificação molecular 653 $aImpedimento taxonômico 653 $aOligoqueta 700 1 $aROSA, M. G. da 700 1 $aSCHUHLI, G. 700 1 $aJAMES, S. W. 700 1 $aBARTZ, M. L. C. 700 1 $aNADOLNY, H. 700 1 $aBUSCH, E. 700 1 $aBROWN, G. G.
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