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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/01/2019 |
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Data da última atualização: |
24/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MELO, J. O.; MARTINS, L. G. C.; BARROS, B. de A.; PIMENTA, M. R.; LANA, U. G. de P.; DUARTE, C. E. M.; PASTINA, M. M.; GUIMARÃES, C. T.; SCHAFFERT, R. E.; KOCHIAN, L. V.; FONTES, E. P. B.; MAGALHAES, J. V. de. |
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Afiliação: |
Janaina O. Melo, Universidade Federal de Minas Gerais; Laura G. C. Martins, Universidade Federal de Viçosa; BEATRIZ DE ALMEIDA BARROS, CNPMS; Maiana R. Pimenta, Universidade Federal de Viçosa; UBIRACI GOMES DE PAULA LANA, CNPMS; Christiane E. M. Duarte, Universidade Federal de Viçosa; MARIA MARTA PASTINA, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; ROBERT EUGENE SCHAFFERT, CNPMS; Leon V. Kochian, University of Saskatchewan, Canada; Elizabeth P. B. Fontes, Universidade Federal de Viçosa; JURANDIR VIEIRA DE MAGALHAES, CNPMS. |
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Título: |
Repeat variants for the SbMATE transporter protect sorghum roots from aluminum toxicity by transcriptional interplay in cis and trans. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Proceedings of the National Academy of Sciences of the United States of America, Washington, v. 116, n. 1, p. 313-318, 2019. |
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DOI: |
10.1073/pnas.1808400115 |
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Idioma: |
Inglês |
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Notas: |
Publicado online em 13 dez. 2018. |
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Conteúdo: |
Acidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespreadand are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by factors located away from SbMATE. Using expressionQTL mapping and expression genome-wide association mapping, we establish that SbMATE transcription is controlled in a bipartite fashion,primarily incis butalsointrans.Multiallelicpromotertransactivation and ChIP analyses demonstrated that intermolecular effects on SbMATE expression arise from a WRKY and a zinc fingerDHHC transcription factor (TF) that bind to and trans-activate the SbMATE promoter. A haplotype analysis in sorghum RILs indicates that the TFs influence SbMATE expression and Al tolerance. Variation in SbMATE expression likely results from changes in tandemly repeated cis sequences flanking a transposable element (a miniature inverted repeattransposable element)insertion inthe SbMATE promoter, which are recognized by the Al3+-responsive TFs. According to our model, repeat expansion in Al-tolerant genotypes increases TF recruitment and, hence, SbMATE expression, which is, in turn, lower in Al-sensitive genetic backgrounds as a result of lower TF expression and fewer binding sites. We thus show that even dominant cis regulation of an agronomically important gene can be subjected to precise intermolecular finetuning. These concerted cis/trans interactions, which allow the plant to sense and respond to environmental cues, such as Al3+ toxicity, can now be used to increase yields and food security on acidic soils. MenosAcidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespreadand are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by factors located away from SbMATE. Using expressionQTL mapping and expression genome-wide association mapping, we establish that SbMATE transcription is controlled in a bipartite fashion,primarily incis butalsointrans.Multiallelicpromotertransactivation and ChIP analyses demonstrated that intermolecular effects on SbMATE expression arise from a WRKY and a zinc fingerDHHC transcription factor (TF) that bind to and trans-activate the SbMATE promoter. A haplotype analysis in sorghum RILs indicates that the TFs influence SbMATE expression and Al tolerance. Variation in SbMATE expression likely results from changes in tandemly repeated cis sequences flanking a transposable element (a miniature inverted repeattransposable element)insertion inthe SbMATE promoter, which are recognized by the Al3+-responsive TFs. According to our model, repeat expansion in Al-tolerant genotypes increases TF recruitment and, hence, SbMATE expression, which is, in turn, lower in Al-sensitive genetic backgrounds as a result of lower TF expression and fewer binding sites. We thus show that even dominant c... Mostrar Tudo |
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Palavras-Chave: |
Estresse abiótico; Regulação transcricional; Toxicidade do alumínio. |
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Thesagro: |
Gene; Genética. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02927naa a2200337 a 4500
001 2103451
005 2020-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1073/pnas.1808400115$2DOI
100 1 $aMELO, J. O.
245 $aRepeat variants for the SbMATE transporter protect sorghum roots from aluminum toxicity by transcriptional interplay in cis and trans.$h[electronic resource]
260 $c2019
500 $aPublicado online em 13 dez. 2018.
520 $aAcidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespreadand are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by factors located away from SbMATE. Using expressionQTL mapping and expression genome-wide association mapping, we establish that SbMATE transcription is controlled in a bipartite fashion,primarily incis butalsointrans.Multiallelicpromotertransactivation and ChIP analyses demonstrated that intermolecular effects on SbMATE expression arise from a WRKY and a zinc fingerDHHC transcription factor (TF) that bind to and trans-activate the SbMATE promoter. A haplotype analysis in sorghum RILs indicates that the TFs influence SbMATE expression and Al tolerance. Variation in SbMATE expression likely results from changes in tandemly repeated cis sequences flanking a transposable element (a miniature inverted repeattransposable element)insertion inthe SbMATE promoter, which are recognized by the Al3+-responsive TFs. According to our model, repeat expansion in Al-tolerant genotypes increases TF recruitment and, hence, SbMATE expression, which is, in turn, lower in Al-sensitive genetic backgrounds as a result of lower TF expression and fewer binding sites. We thus show that even dominant cis regulation of an agronomically important gene can be subjected to precise intermolecular finetuning. These concerted cis/trans interactions, which allow the plant to sense and respond to environmental cues, such as Al3+ toxicity, can now be used to increase yields and food security on acidic soils.
650 $aGene
650 $aGenética
653 $aEstresse abiótico
653 $aRegulação transcricional
653 $aToxicidade do alumínio
700 1 $aMARTINS, L. G. C.
700 1 $aBARROS, B. de A.
700 1 $aPIMENTA, M. R.
700 1 $aLANA, U. G. de P.
700 1 $aDUARTE, C. E. M.
700 1 $aPASTINA, M. M.
700 1 $aGUIMARÃES, C. T.
700 1 $aSCHAFFERT, R. E.
700 1 $aKOCHIAN, L. V.
700 1 $aFONTES, E. P. B.
700 1 $aMAGALHAES, J. V. de
773 $tProceedings of the National Academy of Sciences of the United States of America, Washington$gv. 116, n. 1, p. 313-318, 2019.
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Embrapa Milho e Sorgo (CNPMS) |
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| 1. |  | TORRES, T. R; LUDKE, M. do C. M. M.; RABELLO, C. B. V.; LUDKE, J. V.; SOUZA, E. J. O. de; FARIA, M. A. M. de; COSTA, A. A. G.; LIMA, M. R. de; ANDRADE, E. M. de S. R. de; ASSUNÇÃO FILHO, A. F. Uso de diferentes formas físicas com inclusão de milheto (Pennisetum glaucum) em dietas para frangos de corte In: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UFRPE, 9; SEMANA NACIONAL DE CIÊNCIA E TECNOLOGIA, 6; 2009, Recife. Anais.. Recife: UFRPE, 2009. 1 CD-ROM. Projeto/Plano de Ação: 16.00.30004-00| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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