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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
30/11/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIAS, J.; MARCONDES, M. I.; SOUZA, S. M. de; SILVA, B. C. da M. e; NORONHA, M. F.; RESENDE, R. T.; MACHADO, F. S.; MANTOVANI, H. C.; DILL-MCFARLAND, K. A.; SUEN, G. |
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Afiliação: |
JULIANA DIAS, UFV / CAPES; MARCOS INÁCIO MARCONDES, UFV; SHIRLEY MOTTA DE SOUZA, CAPES; BARBARA CARDOSO DA MATA E SILVA, Universidade José do Rosário Vellano, Alfenas; MELLINE FONTES NORONHA, UNICAMP; RAFAEL TASSINARI RESENDE, UFV; FERNANDA SAMARINI MACHADO, CNPGL; HILÁRIO CUQUETTO MANTOVANI, UFV; KIMBERLY A. DILL-MCFARLAND, University of Wisconsin-Madison, Madison; GARRET SUEN, University of Wisconsin-Madison, Madison. |
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Título: |
Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, v. 84, n. 9, p. e02675-17, 2018. |
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DOI: |
10.1128/AEM.02675-17 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Microbial communities play critical roles in the gastrointestinal tracts (GIT) of preruminant calves by influencing performance and health. However, little is known about the establishment of microbial communities in the calf GIT or their dynamics during development. In this study, next-generation sequencing was used to assess changes in the bacterial communities of the rumen, jejunum, cecum, and colon in 26 crossbred calves at four developmental stages (7, 28, 49, and 63 days old). Alpha diversity differed among GIT regions with the lowest diversity and evenness in the jejunum, whereas no changes in alpha diversity were observed across developmental stage. Beta diversity analysis showed both region and age effects, with low numbers of operational taxonomic units (OTUs) shared between regions within a given age group or between ages in a given region. Taxonomic analysis revealed that several taxa coexisted in the rumen, jejunum, cecum, and colon but that their abundances differed considerably by GIT region and age. As calves aged, we observed lower abundances of taxa such as Bacteroides, Parabacteroides, and Paraprevotella with higher abundances of Bulleidia and Succiniclasticum in the rumen. The jejunum also displayed taxonomic changes with increases in Clostridiaceae and Turicibacter taxa in older calves. In the lower gut, taxa such as Lactobacillus, Blautia, and Faecalibacterium decreased and S24-7, Paraprevotella, and Prevotella increased as calves aged. These data support a model whereby early and successive colonization by bacteria occurs across the GIT of calves and provides insights into the temporal dynamics of the GIT microbiota of dairy calves during preweaning development.IMPORTANCE The gastrointestinal tracts (GIT) of ruminants, such as dairy cows, house complex microbial communities that contribute to their overall health and support their ability to produce milk. For example, the rumen microbiota converts feed into usable nutrients, while the jejunal microbiota provides access to protein. Thus, establishing a properly functioning GIT microbiota in dairy calves is critical to their productivity as adult cows. However, little is known about the establishment, maintenance, and dynamics of the calf GIT microbiota in early life. In this study, we evaluated the bacterial communities in the rumen, jejunum, cecum, and colon in dairy calves across preweaning development and show that they are highly variable early on in life before transitioning to a stable community. Understanding the dairy calf GIT microbiota has implications for ensuring proper health during early life and will aid in efforts to develop strategies for improving downstream production. MenosAbstract Microbial communities play critical roles in the gastrointestinal tracts (GIT) of preruminant calves by influencing performance and health. However, little is known about the establishment of microbial communities in the calf GIT or their dynamics during development. In this study, next-generation sequencing was used to assess changes in the bacterial communities of the rumen, jejunum, cecum, and colon in 26 crossbred calves at four developmental stages (7, 28, 49, and 63 days old). Alpha diversity differed among GIT regions with the lowest diversity and evenness in the jejunum, whereas no changes in alpha diversity were observed across developmental stage. Beta diversity analysis showed both region and age effects, with low numbers of operational taxonomic units (OTUs) shared between regions within a given age group or between ages in a given region. Taxonomic analysis revealed that several taxa coexisted in the rumen, jejunum, cecum, and colon but that their abundances differed considerably by GIT region and age. As calves aged, we observed lower abundances of taxa such as Bacteroides, Parabacteroides, and Paraprevotella with higher abundances of Bulleidia and Succiniclasticum in the rumen. The jejunum also displayed taxonomic changes with increases in Clostridiaceae and Turicibacter taxa in older calves. In the lower gut, taxa such as Lactobacillus, Blautia, and Faecalibacterium decreased and S24-7, Paraprevotella, and Prevotella increased as calves aged. These d... Mostrar Tudo |
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Palavras-Chave: |
16S rRNA; Dairy calf; Gastrointestinal tract; Microbiota. |
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Thesagro: |
Rúmen. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03614naa a2200301 a 4500
001 2100503
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1128/AEM.02675-17$2DOI
100 1 $aDIAS, J.
245 $aBacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development.$h[electronic resource]
260 $c2018
520 $aAbstract Microbial communities play critical roles in the gastrointestinal tracts (GIT) of preruminant calves by influencing performance and health. However, little is known about the establishment of microbial communities in the calf GIT or their dynamics during development. In this study, next-generation sequencing was used to assess changes in the bacterial communities of the rumen, jejunum, cecum, and colon in 26 crossbred calves at four developmental stages (7, 28, 49, and 63 days old). Alpha diversity differed among GIT regions with the lowest diversity and evenness in the jejunum, whereas no changes in alpha diversity were observed across developmental stage. Beta diversity analysis showed both region and age effects, with low numbers of operational taxonomic units (OTUs) shared between regions within a given age group or between ages in a given region. Taxonomic analysis revealed that several taxa coexisted in the rumen, jejunum, cecum, and colon but that their abundances differed considerably by GIT region and age. As calves aged, we observed lower abundances of taxa such as Bacteroides, Parabacteroides, and Paraprevotella with higher abundances of Bulleidia and Succiniclasticum in the rumen. The jejunum also displayed taxonomic changes with increases in Clostridiaceae and Turicibacter taxa in older calves. In the lower gut, taxa such as Lactobacillus, Blautia, and Faecalibacterium decreased and S24-7, Paraprevotella, and Prevotella increased as calves aged. These data support a model whereby early and successive colonization by bacteria occurs across the GIT of calves and provides insights into the temporal dynamics of the GIT microbiota of dairy calves during preweaning development.IMPORTANCE The gastrointestinal tracts (GIT) of ruminants, such as dairy cows, house complex microbial communities that contribute to their overall health and support their ability to produce milk. For example, the rumen microbiota converts feed into usable nutrients, while the jejunal microbiota provides access to protein. Thus, establishing a properly functioning GIT microbiota in dairy calves is critical to their productivity as adult cows. However, little is known about the establishment, maintenance, and dynamics of the calf GIT microbiota in early life. In this study, we evaluated the bacterial communities in the rumen, jejunum, cecum, and colon in dairy calves across preweaning development and show that they are highly variable early on in life before transitioning to a stable community. Understanding the dairy calf GIT microbiota has implications for ensuring proper health during early life and will aid in efforts to develop strategies for improving downstream production.
650 $aRúmen
653 $a16S rRNA
653 $aDairy calf
653 $aGastrointestinal tract
653 $aMicrobiota
700 1 $aMARCONDES, M. I.
700 1 $aSOUZA, S. M. de
700 1 $aSILVA, B. C. da M. e
700 1 $aNORONHA, M. F.
700 1 $aRESENDE, R. T.
700 1 $aMACHADO, F. S.
700 1 $aMANTOVANI, H. C.
700 1 $aDILL-MCFARLAND, K. A.
700 1 $aSUEN, G.
773 $tApplied and Environmental Microbiology$gv. 84, n. 9, p. e02675-17, 2018.
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