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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido; Embrapa Uva e Vinho. |
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Data corrente: |
29/10/2018 |
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Data da última atualização: |
08/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FALAVGNA, V. da S.; PORTO, D. D.; MIOTTO, Y. E.; SANTOS, H. P. dos; OLIVEIRA, P. R. D. de; PINHEIRO, M M.; GIANCARLO PASQUALI, G.; REVERS, L. F. |
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Afiliação: |
VÍTOR DA SILVEIRA FALAVIGNA, Universidade Federal do Rio Grande do Sul; DIOGO DENARDI PORTO, CPATSA; YOHANNA EVELYN MIOTTO; HENRIQUE PESSOA DOS SANTOS, CNPUV; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; MÁRCIA MARGIS-PINHEIRO, Universidade Federal do Rio Grande do Sul; GIANCARLO PASQUALI, Universidade Federal do Rio Grande do Sul; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Experimental Botany, v. 69, n. 5, p. 1247- 1259, 2018. |
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DOI: |
10.1093/jxb/erx451 |
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Idioma: |
Inglês |
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Conteúdo: |
Galactinol synthase (GolS) is a key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs), which play roles in carbon storage, signal transduction, and osmoprotection. The present work assessed the evolutionary history of GolS genes across the Rosaceae using several bioinformatic tools. Apple (Malus × domestica) GolS genes were transcriptionally characterized during bud dormancy, in parallel with galactinol and raffinose measurements. Additionally, MdGolS2, a candidate to regulate seasonal galactinol and RFO content during apple bud ormancy, was functionally characterized in Arabidopsis. Evolutionary analyses revealed that whole genome duplications have driven GolS gene evolution and diversification in Rosaceae speciation. The strong purifying selection identified in duplicated GolS genes suggests that differential gene expression might define gene function better than protein structure. Interestingly, MdGolS2 was differentially expressed during bud dormancy, concomitantly with the highest galactinol and raffinose levels. One of the intrinsic adaptive features of bud dormancy is limited availability of free water; therefore, we generated transgenic Arabidopsis plants expressing MdGolS2. They showed higher galactinol and raffinose contents and increased tolerance to water deficit. Our results suggest that MdGolS2 is the major GolS responsible for RFO accumulation during apple dormancy, and these carbohydrates help to protect dormant buds against limited water supply. Keywords: Apple, bud dormancy, galactinol synthase, Malus × domestica, raffinose family oligosaccharides, tolerance to water deficit, transgenic expression, whole-genome duplication. MenosGalactinol synthase (GolS) is a key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs), which play roles in carbon storage, signal transduction, and osmoprotection. The present work assessed the evolutionary history of GolS genes across the Rosaceae using several bioinformatic tools. Apple (Malus × domestica) GolS genes were transcriptionally characterized during bud dormancy, in parallel with galactinol and raffinose measurements. Additionally, MdGolS2, a candidate to regulate seasonal galactinol and RFO content during apple bud ormancy, was functionally characterized in Arabidopsis. Evolutionary analyses revealed that whole genome duplications have driven GolS gene evolution and diversification in Rosaceae speciation. The strong purifying selection identified in duplicated GolS genes suggests that differential gene expression might define gene function better than protein structure. Interestingly, MdGolS2 was differentially expressed during bud dormancy, concomitantly with the highest galactinol and raffinose levels. One of the intrinsic adaptive features of bud dormancy is limited availability of free water; therefore, we generated transgenic Arabidopsis plants expressing MdGolS2. They showed higher galactinol and raffinose contents and increased tolerance to water deficit. Our results suggest that MdGolS2 is the major GolS responsible for RFO accumulation during apple dormancy, and these carbohydrates help to protect dormant buds against limit... Mostrar Tudo |
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Palavras-Chave: |
Dormência das gemas; Galactinol sintase; Oligossacarídeos da família da rafinose. |
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Thesagro: |
Dormência; Maçã; Malus Domestica. |
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Thesaurus Nal: |
Apples. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02658naa a2200301 a 4500
001 2098356
005 2018-11-08
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1093/jxb/erx451$2DOI
100 1 $aFALAVGNA, V. da S.
245 $aEvolutionary diversification of galactinol synthases in Rosaceae$badaptive roles of galactinol and raffinose during apple bud dormancy.$h[electronic resource]
260 $c2018
520 $aGalactinol synthase (GolS) is a key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs), which play roles in carbon storage, signal transduction, and osmoprotection. The present work assessed the evolutionary history of GolS genes across the Rosaceae using several bioinformatic tools. Apple (Malus × domestica) GolS genes were transcriptionally characterized during bud dormancy, in parallel with galactinol and raffinose measurements. Additionally, MdGolS2, a candidate to regulate seasonal galactinol and RFO content during apple bud ormancy, was functionally characterized in Arabidopsis. Evolutionary analyses revealed that whole genome duplications have driven GolS gene evolution and diversification in Rosaceae speciation. The strong purifying selection identified in duplicated GolS genes suggests that differential gene expression might define gene function better than protein structure. Interestingly, MdGolS2 was differentially expressed during bud dormancy, concomitantly with the highest galactinol and raffinose levels. One of the intrinsic adaptive features of bud dormancy is limited availability of free water; therefore, we generated transgenic Arabidopsis plants expressing MdGolS2. They showed higher galactinol and raffinose contents and increased tolerance to water deficit. Our results suggest that MdGolS2 is the major GolS responsible for RFO accumulation during apple dormancy, and these carbohydrates help to protect dormant buds against limited water supply. Keywords: Apple, bud dormancy, galactinol synthase, Malus × domestica, raffinose family oligosaccharides, tolerance to water deficit, transgenic expression, whole-genome duplication.
650 $aApples
650 $aDormência
650 $aMaçã
650 $aMalus Domestica
653 $aDormência das gemas
653 $aGalactinol sintase
653 $aOligossacarídeos da família da rafinose
700 1 $aPORTO, D. D.
700 1 $aMIOTTO, Y. E.
700 1 $aSANTOS, H. P. dos
700 1 $aOLIVEIRA, P. R. D. de
700 1 $aPINHEIRO, M M.
700 1 $aGIANCARLO PASQUALI, G.
700 1 $aREVERS, L. F.
773 $tJournal of Experimental Botany$gv. 69, n. 5, p. 1247- 1259, 2018.
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Embrapa Semiárido (CPATSA) |
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| 1. |  | OTUKA, A. J. G.; ALMEIDA, J. M. P.; TRIBUZI, V.; CARDOSO, M. R.; HERNANDES, A. C.; CORREA, D. S.; MENDONÇA, C. R. Femtosecond lasers for processing glassy and polymeric materials. Materials Research, São Carlos, v. 112, p. 203-317, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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| 2. | | OTUKA, A. J. G. ALMEIDA, J. M. P. TRIBUZI, V. CARDOSO, M. R. HERNANDES, A. C.; CORREA, D. S.; MENDONÇA, C. R. Femtosecond lasers for processing glassy and polymeric materials. Materials Research, São Carlos, v. 17, n. 2, p. 352-358, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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