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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
28/09/2018 |
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Data da última atualização: |
28/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GRESSLER, V.; FEDDERN, V.; PEIXOTO, J. de O.; LEDUR, M. C.; DALLA COSTA, O. A.; LIMA, G. J. M. M. de. |
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Afiliação: |
VANESSA GRESSLER, CNPSA; VIVIAN FEDDERN, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; MONICA CORREA LEDUR, CNPSA; OSMAR ANTONIO DALLA COSTA, CNPSA; GUSTAVO JULIO MELLO M DE LIMA, CNPSA. |
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Título: |
Application of enzyme digestion and deconjugation followed by quick, easy, cheap, effective, rugged, safe extraction and liquid chromatography?tandem mass spectrometry methodology to determine ractopamine residue in pork. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Food Protection, v. 81, n.8, p. 1258-1263, 2018. |
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DOI: |
10.4315/0362-028X.JFP-17-526 |
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Idioma: |
Inglês |
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Conteúdo: |
A new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography?tandem mass spectrometry (LC-MS/MS). In brief, the samples were digested with protease and then deconjugated with b-glucuronidase enzyme; they were then subjected to extraction and cleanup by QuEChERS and underwent sequential analysis by LC-MS/MS. The method performance was evaluated in accordance to the validation guidelines regulated by the Brazilian Ministry of Agriculture and Food Supply. The limit of detection was 0.15 lg/kg and limit of quantification was 0.5 lg/kg. When the method was applied to real samples, ractopamine residue was found in concentrations (up to 7.86 lg/kg) below international recommendation limits up to 10 lg/kg. The method is sensitive, accurate, quick, simple, and suitable for routine analysis; therefore, it is a monitoring tool that may be adopted by laboratories to achieve compliance levels. |
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Palavras-Chave: |
B-agonista; Carne suína; Method validation; Método de validação; Músculo suíno; Ractopamina; Swine muscle. |
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Thesagro: |
Carcaça; Cromatografia; Digestão; Espectrometria; Hidrolise; Nutrição Animal; Suíno. |
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Thesaurus Nal: |
Animal nutrition; Digestion; Hydrolysis; Liquid chromatography; Mass spectrometry; Methodology; Ractopamine; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
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005 2018-09-28
008 2018 bl uuuu u00u1 u #d
024 7 $a10.4315/0362-028X.JFP-17-526$2DOI
100 1 $aGRESSLER, V.
245 $aApplication of enzyme digestion and deconjugation followed by quick, easy, cheap, effective, rugged, safe extraction and liquid chromatography?tandem mass spectrometry methodology to determine ractopamine residue in pork.$h[electronic resource]
260 $c2018
520 $aA new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography?tandem mass spectrometry (LC-MS/MS). In brief, the samples were digested with protease and then deconjugated with b-glucuronidase enzyme; they were then subjected to extraction and cleanup by QuEChERS and underwent sequential analysis by LC-MS/MS. The method performance was evaluated in accordance to the validation guidelines regulated by the Brazilian Ministry of Agriculture and Food Supply. The limit of detection was 0.15 lg/kg and limit of quantification was 0.5 lg/kg. When the method was applied to real samples, ractopamine residue was found in concentrations (up to 7.86 lg/kg) below international recommendation limits up to 10 lg/kg. The method is sensitive, accurate, quick, simple, and suitable for routine analysis; therefore, it is a monitoring tool that may be adopted by laboratories to achieve compliance levels.
650 $aAnimal nutrition
650 $aDigestion
650 $aHydrolysis
650 $aLiquid chromatography
650 $aMass spectrometry
650 $aMethodology
650 $aRactopamine
650 $aSwine
650 $aCarcaça
650 $aCromatografia
650 $aDigestão
650 $aEspectrometria
650 $aHidrolise
650 $aNutrição Animal
650 $aSuíno
653 $aB-agonista
653 $aCarne suína
653 $aMethod validation
653 $aMétodo de validação
653 $aMúsculo suíno
653 $aRactopamina
653 $aSwine muscle
700 1 $aFEDDERN, V.
700 1 $aPEIXOTO, J. de O.
700 1 $aLEDUR, M. C.
700 1 $aDALLA COSTA, O. A.
700 1 $aLIMA, G. J. M. M. de
773 $tJournal of Food Protection$gv. 81, n.8, p. 1258-1263, 2018.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
18/03/2011 |
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Data da última atualização: |
19/12/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BENKO-ISEPPON, A. M.; KIDO, E. A.; PANDOLFI, V.; BARBOSA, P. K. A; BELARMINO, L. C.; MONTE, S. J. H. do; BRANDÃO, R. M. S. de; ARAUJO, A. de S.; CASTRO, J. A. F. de; SOARES-CAVALCANTI, N. da M.; SILVA, A. R. da; CALSA JUNIOR, T.; ROCHA, M. de M.; WINTER, P.; KAHL, G.; ROTTER, B.; HORRES, R.; MOLINA, C.; JUNGMANN, R.; AMORIM, L. L. B.; ONOFRE, A. V. C.; FERREIRA-NETO, J. R. C.; GRANJEIRO, T. B.; LIMA, A. S.; LOBO, M. D. P.; HOULLOU-KIDO, L. M.; CARVALHO, R. de; WANDERLEY-NOGUEIRA, A. C.; BARROS, P. dos S.; VIEIRA-MELLO, G. S.; BRASILEIRO-VIDAL, A. C.; BORTOLETI, K. C. de A.; PEDROSA-HARAND, A.; ANDRADE, P. P. de; ANDRADE, G. P. de; PIO-RIBEIRO, G.; SITTOLIN, I. M.; FREIRE FILHO, F. R. |
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Afiliação: |
ANA M. BENKO-ISEPPON, UFPE; EDERSON A. KIDO, UFPE; VALESCA PANDOLFI, UFPE; PEDRANNE K. A. BARBOSA, UFPE; LUIZ C. BELARMINO, UFPE; SEMIRAMIS J. H. DO MONTE, UFPI; RAFAEL M. S. DE S. BRANDÃO, UFPI; ANAREGINA DE S. ARAUJO, UFPI; JOSE A. F. DE CASTRO, UFPI; NINA DA M. SOARES-CAVALCANTI, UFPE; ADRIANO R. DA SILVA, UFPE; TERCILIO CALSA JUNIOR, UFPE; MAURISRAEL DE MOURA ROCHA, CPAMN; PETER WINTER, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; GUNTER KAHL, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; BJORN ROTTER, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; RALF HORRES, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; CARLOS MOLINA, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; RUTH JUNGMANN, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; LIDIANE L. B. AMORIM, UFPE; ALBERTO V. C. ONOFRE, UFPE; JOSE R. C. FERREIRA-NETO, UFPE; THALLES B. GRANJEIRO, UFC; ADRIANA S. LIMA, UFC; MARINA D. P. LOBO, UFC; LAUREEN M. HOULLOU-KIDO, CETENE; REGINALDO DE CARVALHO, UFPE; ANA C. WANDERLEY-NOGUEIRA, UFPE; PETRA DOS S. BARROS, UFPE; GABRIELA S. VIEIRA-MELLO, UFPE; ANA C. BRASILEIRO-VIDAL, UFPE; KYRIA C. DE A. BORTOLETI, UFPE; ANDREA PEDROSA-HARAND, UFPE; PAULO P. DE ANDRADE, UFPE; GENIRA P. DE ANDRADE, UFPE; GILVAN PIO-RIBEIRO, UFRPE; ILZA MARIA SITTOLIN, CPAMN; FRANCISCO RODRIGUES FREIRE FILHO, CPAMN. |
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Título: |
Brazilian cowpea transcriptome project: over 20 million expressed sequence tags to understand salinity and virus resistance. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010. Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. |
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Páginas: |
p. 97-98. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Feijão-caupi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30057/1/Brazilian0001.pdf
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Marc: |
LEADER 01714nam a2200565 a 4500
001 1881607
005 2023-12-19
008 2010 bl uuuu u00u1 u #d
100 1 $aBENKO-ISEPPON, A. M.
245 $aBrazilian cowpea transcriptome project$bover 20 million expressed sequence tags to understand salinity and virus resistance.
260 $aIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010. Fortaleza. Programa e resumos. Brasília, DF: SBBiotec$c2010
300 $ap. 97-98.
653 $aFeijão-caupi
700 1 $aKIDO, E. A.
700 1 $aPANDOLFI, V.
700 1 $aBARBOSA, P. K. A
700 1 $aBELARMINO, L. C.
700 1 $aMONTE, S. J. H. do
700 1 $aBRANDÃO, R. M. S. de
700 1 $aARAUJO, A. de S.
700 1 $aCASTRO, J. A. F. de
700 1 $aSOARES-CAVALCANTI, N. da M.
700 1 $aSILVA, A. R. da
700 1 $aCALSA JUNIOR, T.
700 1 $aROCHA, M. de M.
700 1 $aWINTER, P.
700 1 $aKAHL, G.
700 1 $aROTTER, B.
700 1 $aHORRES, R.
700 1 $aMOLINA, C.
700 1 $aJUNGMANN, R.
700 1 $aAMORIM, L. L. B.
700 1 $aONOFRE, A. V. C.
700 1 $aFERREIRA-NETO, J. R. C.
700 1 $aGRANJEIRO, T. B.
700 1 $aLIMA, A. S.
700 1 $aLOBO, M. D. P.
700 1 $aHOULLOU-KIDO, L. M.
700 1 $aCARVALHO, R. de
700 1 $aWANDERLEY-NOGUEIRA, A. C.
700 1 $aBARROS, P. dos S.
700 1 $aVIEIRA-MELLO, G. S.
700 1 $aBRASILEIRO-VIDAL, A. C.
700 1 $aBORTOLETI, K. C. de A.
700 1 $aPEDROSA-HARAND, A.
700 1 $aANDRADE, P. P. de
700 1 $aANDRADE, G. P. de
700 1 $aPIO-RIBEIRO, G.
700 1 $aSITTOLIN, I. M.
700 1 $aFREIRE FILHO, F. R.
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