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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. |
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Afiliação: |
Helena F. R. de A. Saraiva, UFF; Ribrio I. T. P. Batista, UFF; Vivian A. P. Alfradique, UFF; Pedro H. N. Pinto, UFF; Lilian S. Ribeiro, UFF; Clara S. Oliveira, UFF; Joanna M. G. de Souza-Fabjan, UFF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Z. Brandão, UFF. |
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Título: |
L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, jan. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production; Santa ines. |
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Thesagro: |
Ovis Aries. |
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Thesaurus Nal: |
cryopreservation; gene expression. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02970naa a2200289 a 4500
001 2085659
005 2023-01-27
008 2018 bl uuuu u00u1 u #d
100 1 $aSARAIVA, H. F. R. de A.
245 $aL-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression.$h[electronic resource]
260 $c2018
520 $aAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
650 $acryopreservation
650 $agene expression
650 $aOvis Aries
653 $aIn vivo embryo production
653 $aSanta ines
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDAO, F. Z.
773 $tTheriogenology$gv. 105, p. 150-157, jan. 2018.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 38 | |
| 1. |  | ZILLI, J. E.; CAMPO, R. J.; RIBEIRO, K. G.; GIANLUPPI, V.; SMIDERLE, O. J.; HUNGRIA, M. Eficiência das estirpes de Bradyrhizobium recomendadas no Brasil para a fixação biológica de nitrogênio (FBN) na cultura da soja, cultivada no Cerrado de Roraima. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 182. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 2. | | ZILLI, J. E.; CAMPO, R. J.; RIBEIRO, K. G.; GIANLUPPI, V.; SMIDERLE, O. J.; HUNGRIA, M. Eficiência da fixação biológica de nitrogênio na soja em área primeiro ano de cultivo, no cerrado de Roraima. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 181-182. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 5. | | ZILLI, J. E.; RIBEIRO, K. G.; CAMPO, R. J.; HUNGRIA, M. Efeito de fungicidas à base de carbendazim+thiram e carboxin+thiram na nodulação e rendimento de grãos na cultura da soja. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR, UEL, 2008. 1 CD-ROM. Organizado por: Adilson de Oliveira Júnior, Regina Maria Villas Bôas de Campos Leite, César de Castro, Fábio Álvares de Oliveira; Odilon Ferreira Saraiva.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | ZILLI, J. E.; RIBEIRO, K. G.; CAMPO, R. J.; HUNGRIA, M. Influence of fungicide seed treatment on soybean nodulation and grain yield. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 33, n. 4, p. 917-923, July/Aug. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Roraima; Embrapa Soja. |
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| 11. | | GOMIDE, C. A. de M.; ANJOS, A. J.; RIBEIRO, K. G.; PACIULLO, D. S. C.; MORENZ, M. J. F. Canopy structure of marandu palisadegrass pasture under rotational stocking strategies. In: ASA, CSSA & SSSA INTERNATIONAL ANNUAL MEETINGS, 2013, Tampa, FL. Water, food, energy & inovation for a sustainable world: abstracts. Madison: American Society of Agronomy, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | SANTOS, V, C.; CHAVES, C. S.; RIBEIRO, K. G.; PEREIRA, O. G.; CECCON, P. R.; GOMIDE, C. A. de M. Morphogenic, structural, and chemical characteristics of Brachiaria grass (Urochloa decumbens Stapf.) pastures in monoculture and intercropped with forage peanut under two grazing intensities. Semina. Ciências Agrárias, v. 44, n. 1, p. 375-392, jan./fev. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | CHAVES, C. S.; RIBEIRO, K. G.; GOMIDE, C. A. de M.; CAMPOS, A. F.; PEREIRA, O. G.; COSTA, I. DE A. Fibra em detergente neutro e matéria seca potencialmente digeríveis de clones de capim-elefante anão sob estratégias de lotação intermitente. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém. O Desenvolvimento da produção animal e a responsabilidade frente a novos desafios. Viçosa, MG: Sociedade Brasileira de Zootecnia, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | GOMIDE, C. A. de M.; SILVA, R. B.; RIBEIRO, K. G.; LEDO, F. J. da S.; PACIULLO, D. S. C.; BARRADAS, E. M. Evaluation of new dwarf elephant grass genotypes for grazing. In: INTERNATIONAL GRASSLAND CONGRESS, 25., 2023, Kentucky. Grasslands for animal, soil and human health: proceedings. Berea: American Forage and Grassland Council, 2023.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | ANJOS, A. J. DOS; GOMIDE, C. A. de M.; RIBEIRO, K. G.; MADEIRO, A. S.; MATA E SILVA, B. C. DA; MORENZ, M. J. F. Composição morfológica do resíduo do capim-marandu submetido a estratégias de lotação rotacionada. In: MOSTRA DE PESQUISA E EXTENSÃO EM PECUÁRIA DE CORTE NO NORTE DE MINAS GERAIS, 2., 2013, Montes Claros. Anais... Montes Claros: [s.n.], 2013,| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | ANJOS, A. J. DOS; GOMIDE, C. A. de M.; RIBEIRO, K. G.; OLIVEIRA, A. M. S. DE; MORENZ, M. J. F.; PACIULLO, D. S. C. Produtividade e composição morfológica do capim-marandu sob lotação intermitente com período de descanso variável ou fixo. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília. A produção animal no mundo em transformação: anais. Brasília, DF: SBZ, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | RIBEIRO, K. G.; BARAÚNA, A. C.; PEREIRA, G. M. D.; FERNANDES JÚNIOR, P. I.; VITAL, M. J. S.; ZILLI, J. E. Fungos simbióticos associados ao sistema radicular de arroz silvestre. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 20. | | ANJOS, A. J. DOS; GOMIDE, C. A. de M.; RIBEIRO, K. G.; MADEIRO, A. S.; MATA E SILVA, B. C. DA; PACIULLO, D. S. C. Relação lâmina/colmo do capim-marandu submetido a estratégias de lotação rotacionada. In: MOSTRA DE PESQUISA E EXTENSÃO EM PECUÁRIA DE CORTE NO NORTE DE MINAS GERAIS, 2., 2013, Montes Claros. Anais... Montes Claros: [s.n.], 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 38 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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