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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/12/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, R. L. da; SOUZA, E. H. de; VIEIRA, L. de J.; PELACANI, C. R.; SOUZA, F. V. D. |
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Afiliação: |
RONILZE LEITE DA SILVA, EFS; EVERTON HILO DE SOUZA, CAPES; LÍVIA DE JESUS VIEIRA, CAPES; CLAUDINÉIA REGINA PELACANI, UEFS; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Cryopreservation of pollen of wild pineapple accessions. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Scientia Horticulturae, v.219, p.326-334, May 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Cryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in vitro germination percentages and production of viable seeds. MenosCryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in... Mostrar Tudo |
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Thesagro: |
Abacaxi. |
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Categoria do assunto: |
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Marc: |
LEADER 02070naa a2200181 a 4500
001 2081351
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008 2017 bl uuuu u00u1 u #d
100 1 $aSILVA, R. L. da
245 $aCryopreservation of pollen of wild pineapple accessions.$h[electronic resource]
260 $c2017
520 $aCryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in vitro germination percentages and production of viable seeds.
650 $aAbacaxi
700 1 $aSOUZA, E. H. de
700 1 $aVIEIRA, L. de J.
700 1 $aPELACANI, C. R.
700 1 $aSOUZA, F. V. D.
773 $tScientia Horticulturae$gv.219, p.326-334, May 2017.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 5 | |
| 1. |  | BRAGA, L. D. da C.; PRAXEDES, V. A.; BRITO, L. F.; PEIXOTO, M. G. C. D.; OLIVEIRA, M. M. N. F. de; PIRES, M. de F. A. Comportamento materno-filial de vacas Guzerá com bezerros mestiços Holandês x Guzerá em rebanhos leiteiros. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | BRAGA, L. D. da C.; PRAXEDES, V. A.; BRITO, L. F.; PEIXOTO, M. G. C. D.; OLIVEIRA, M. M. N. F. de; PIRES, M. de F. A. Influência da presença do urubu (Coragyps atratus) na interação materno filial após o parto. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | AMORIM, L. da S.; TORRES, C. A. A.; SIQUEIRA, L. G. B.; FONSECA, J. F. da; GUIMARÃES, J. D.; CARVALHO, G. R. de; ALVES, N. G.; OLIVEIRA, M. M. N. F. de. Embryo development and follicular status of Toggenburg does fed urea diet. Revista Brasileira de Zootecnia, v. 40, n. 2, p. 277-285, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 4. | | NASCIMENTO, V. A.; TORRES, C. A. A.; OLIVEIRA, M. M. N. F. de; MOURÃO, L. L.; CARNEIRO, C.; DIAS, M.; SOUSA, K. R. S.; OLIVEIRA, F. A.; VIANA, J. H. M. Características biofísicas do Milieu Intérieur uterino em vacas da raça nelore submetidas a protocolos de sincronização de ovulação e estro In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 43., 2006, João Pessoa. Produção animal em biomas tropicais: anais. Viçosa, MG: Sociedade Brasileira de Zootecnia, 2006.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | NASCIMENTO, V. A.; TORRES, C. A. A.; OLIVEIRA, M. M. N. F. de; LOGRADO, M. L.; VIANA, J. H. M.; COSTA, E. P. da; SOUSA, K. R. S.; CARNEIRO, C.; OLIVEIRA, F. A. Oestrous and of the ovulation synchronization, artificial insemination in fixed time and pregnancy values in nelore milk cows. Acta Scientiae Veterinariae, v. 34, s282, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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