| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
29/09/2017 |
|
Data da última atualização: |
10/01/2025 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
|
Afiliação: |
HELENA FABIANA REIS DE ALMEIDA SARAIVA, UNIVERSIDADE FEDERAL FLUMINENSE; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; VIVIAN ANGÉLICO PEREIRA ALFRADIQUE, UNIVERSIDADE FEDERAL FLUMINENSE; PEDRO HENRIQUE NICOLAU PINTO, UNIVERSIDADE FEDERAL FLUMINENSE; LILIAN SANTOS RIBEIRO, UNIVERSIDADE FEDERAL FLUMINENSE; CLARA SLADE OLIVEIRA, CNPGL; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE. |
|
Título: |
l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, Jan. 2018. |
|
DOI: |
https://doi.org/10.1016/j.theriogenology.2017.09.022 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control... Mostrar Tudo |
|
Palavras-Chave: |
Animal embryos; ATP cycle; Drug effects; Mitochondrial genetics; Santa ines; Survival. |
|
Thesagro: |
Ovino. |
|
Thesaurus Nal: |
Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; oocytes; Sheep; Vitrification. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03336naa a2200433 a 4500
001 2076472
005 2025-01-10
008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2017.09.022$2DOI
100 1 $aSARAIVA, H. F. R. de A.
245 $al-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression.$h[electronic resource]
260 $c2017
520 $aAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
650 $aCarnitine
650 $aCell culture
650 $aCryopreservation
650 $aEwes
650 $aGene expression
650 $aIn vitro culture
650 $aoocytes
650 $aSheep
650 $aVitrification
650 $aOvino
653 $aAnimal embryos
653 $aATP cycle
653 $aDrug effects
653 $aMitochondrial genetics
653 $aSanta ines
653 $aSurvival
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tTheriogenology$gv. 105, p. 150-157, Jan. 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 16 | |
| 12. |  | TEIXEIRA, S. C. G.; MOREIRA, J. da C.; FERREIRA, L. F. V.; OLIVEIRA, A. S.; MARQUES, M. R. da C.; PEREZ, D. V. Adsorção e fotoquímica de pireno em solos brasileiros com e sem matéria orgânica. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
|    |
| 13. | | TEIXEIRA, S. C. G.; MOREIRA, J. da C.; FERREIRA, L. F. V.; OLIVEIRA, A. S.; PEREZ, D. V.; MARQUES, M. R. da C. Adição de matéria orgânica em Latossolo Amarelo e seu reflexo na adsorção de pireno. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios. [Viçosa, MG]: SBCS; Fortaleza: UFC, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
| |
| 14. | | TEIXEIRA, S. C. G.; MOREIRA, J. da C.; FERREIRA, L. F. V.; OLIVEIRA, A. S.; PEREZ, D. V.; MARQUES, M. R. da C. Comportamento de um poluente orgânico persistente (POP) com organossolos brasileiros. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 32., 2009, Fortaleza. Químicos para uma potência emergente: resumos. São Paulo: Sociedade Brasileira de Química, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
| |
| 15. | | TEXEIRA, S. C. G.; MOREIRA, J. da C.; FERREIRA, L. F. V.; OLIVEIRA, A. S.; PEREZ, D. V.; MARQUES, M. R. da C. Interação de pireno e fenantreno com argilas em suspensão. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 32., 2009, Fortaleza. Químicos para uma potência emergente: resumos. São Paulo: Sociedade Brasileira de Química, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
| |
| 16. | | GOMES, F. V. A.; SANTOS, A. L. da C. dos; VALLE, A. S. da S.; TEIXEIRA, S. C. G.; CANELA, M. C.; SANTA MARIA, L. C. de; PEREZ, D. V.; MARQUES, M. R. da C. Avaliação da eficiência de argilas como catalisadores para reação de fenton em meio neutro. In: SIMPÓSIO INTERNACIONAL DE MEIO AMBIENTE, 4., 2009, Rio de Janeiro. Resumos expandidos. Rio de Janeiro: UFRJ, 2009. p. 444-448.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
| |
| Registros recuperados : 16 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
