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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
21/09/2017 |
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Data da última atualização: |
21/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEARSON, B. L.; BEARSON, S. M. D.; BRUNELLE, B. W.; BAYLES, D. O.; LEE, I. S.; KICH, J. D. |
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Afiliação: |
BRADLEY L. BEARSON, USDA/ARS/National Laboratory for Agriculture and the Environment; SHAWN M. D. BEARSON, USDA/ARS/National Animal Disease Center; BRIAN W. BRUNELLE, USDA/ARS/National Animal Disease Center; DARRELL O. BAYLES, USDA/ARS/National Animal Disease Center; IN SOO LEE, Hannam University; JALUSA DEON KICH, CNPSA. |
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Título: |
Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Medical Microbiology, v. 66, p. 651-661, 2017. |
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DOI: |
10.1099/jmm.0.000482 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination. Conclusion. Evaluation of the S. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits. MenosAbstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, whic... Mostrar Tudo |
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Thesagro: |
Salmonella Typhimurium; Suinocultura; Vacina; Virologia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02678naa a2200241 a 4500
001 2076069
005 2017-09-21
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1099/jmm.0.000482$2DOI
100 1 $aBEARSON, B. L.
245 $aSalmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine.$h[electronic resource]
260 $c2017
520 $aAbstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination. Conclusion. Evaluation of the S. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits.
650 $aSalmonella Typhimurium
650 $aSuinocultura
650 $aVacina
650 $aVirologia
700 1 $aBEARSON, S. M. D.
700 1 $aBRUNELLE, B. W.
700 1 $aBAYLES, D. O.
700 1 $aLEE, I. S.
700 1 $aKICH, J. D.
773 $tJournal of Medical Microbiology$gv. 66, p. 651-661, 2017.
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| 3. |  | LAGÔA, A. C. G.; MORAES, M. C. B.; BORGES, M.; LAUMANN, R. A. Interação entre percevejos (Heteroptera: Pentatomidae) e os parasitoides de ovos Telenomus podisi e Trissolcus basalis (Hymenoptera: Platygastridae) mediada por rastros químicos. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | SOUZA, L. S.; LIMA, D. D.; SOUSA, A. A. T. C. de; SOUZA, L. M. de; LAGÔA, A. C. G.; PIRES, C. S. S.; SUJII, E. R. Abelhas visitantes florais dos maracujás BRS Mel do Cerrado e BRS Sertão forte no Distrito Federal. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., Gramado. Saúde, ambiente e agricultura: anais. Gramado: SEB, 2018. p. 1286| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | SOUZA, L. S.; PIRES, C. S. S.; LAGÔA, A. C. G.; SOUZA, L. M. de; SOUSA, A. A. T. C. de; FALEIRO, F. G.; SUJII, E. R. Abelhas visitantes florais e potenciais polinizadoras das cultivares de maracujás silvestres. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2019. 30 (Embrapa Recursos Genéticos e Biotecnologia, Boletim de Pesquisa, 348)| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | SILVEIRA, S. da; DIAS, A. M.; LAGOA, A. C. G.; BLASSIOLI-MORAES, M. C.; BORGES, M.; COK, A.; LAUMANN, R. A. Specificity of male responses to female vibratory signals in two Chinavia species (Hemiptera: Pentatomidae) is based on signal structure and narrow temporal parameters. Animal Behavior and Cognition, v. 6, n. 1, p. 1-12, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
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