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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
Data corrente: |
06/07/2017 |
Data da última atualização: |
21/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARTINS, K. B.; FACCIOLI, P. Y.; BONESSO, M. F.; FERNANDES, S.; OLIVEIRA, A. A.; DANTAS, A.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. |
Afiliação: |
Katheryne Benini Martins, UNESP; Patricia Yoshida Faccioli, UNESP; Mariana Fávero Bonesso, UNESP; Simone Fernandes, UNESP; Aline A. Oliveira, UNESP; Ariane Dantas, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Maria de Lourdes Ribeiro de Souza da Cunha, UNESP. |
Título: |
Characteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Journal of Dairy Science, v. 100, n. 3, p. 2184-2195, 2017. |
ISSN: |
0022-0302 |
DOI: |
https://doi.org/10.3168/jds.2016-11583 |
Idioma: |
Inglês |
Conteúdo: |
Coagulase-negative staphylococci (CNS) are among the main responsible agents for mastitis in sheep. Cure rates can be reduced due to several causes, such as those related to virulence factors presented by microorganisms. This study aims at characterizing the virulence and resistance factors to antimicrobial agents in different CNS species isolated from sheep milk. After collecting milk samples, the samples were analyzed and the CNS species were identified. After identification, the susceptibility-sensitivity profile was examined using the disk diffusion technique for 10 antimicrobial agents. The DNA was extracted to detect the presence of the mecA gene, biofilm (icaADBC, bap, and bhp) and toxin genes (sea, seb, sec, sed, tst, and luk-PV) by PCR. Samples carrying toxin genes had their expression assessed using the reverse-transcription PCR technique. The biofilm production was assessed using the adherence method on a polystyrene plate. One hundred twelve CNS samples were isolated, 53 (47.3%) from animals with subclinical mastitis and 59 (52.7%) from healthy animals. Drugs tested have shown to be efficient for most CNS samples. The largest resistance percentage of CNS was found for the penicillin (17.0%) and tetracycline (10.7%) and 4 samples carried the mecA gene. As for the biofilm genes, the icaADBC operon was found in 10 (8.9%) samples, the bap gene was found in 16 (14.3%), and the bhp gene was found in 3 (2.7%). In addition, 69 (61.6%) samples produced biofilm. The survey of toxin genes has shown that 70 (62.5%) samples showed some toxin-encoding gene. However, none of the samples has expressed any of the genes from those toxins studied. MenosCoagulase-negative staphylococci (CNS) are among the main responsible agents for mastitis in sheep. Cure rates can be reduced due to several causes, such as those related to virulence factors presented by microorganisms. This study aims at characterizing the virulence and resistance factors to antimicrobial agents in different CNS species isolated from sheep milk. After collecting milk samples, the samples were analyzed and the CNS species were identified. After identification, the susceptibility-sensitivity profile was examined using the disk diffusion technique for 10 antimicrobial agents. The DNA was extracted to detect the presence of the mecA gene, biofilm (icaADBC, bap, and bhp) and toxin genes (sea, seb, sec, sed, tst, and luk-PV) by PCR. Samples carrying toxin genes had their expression assessed using the reverse-transcription PCR technique. The biofilm production was assessed using the adherence method on a polystyrene plate. One hundred twelve CNS samples were isolated, 53 (47.3%) from animals with subclinical mastitis and 59 (52.7%) from healthy animals. Drugs tested have shown to be efficient for most CNS samples. The largest resistance percentage of CNS was found for the penicillin (17.0%) and tetracycline (10.7%) and 4 samples carried the mecA gene. As for the biofilm genes, the icaADBC operon was found in 10 (8.9%) samples, the bap gene was found in 16 (14.3%), and the bhp gene was found in 3 (2.7%). In addition, 69 (61.6%) samples produced biofilm. The survey... Mostrar Tudo |
Palavras-Chave: |
Coagulase-negative staphylococci; Resistance; Sheep mastitis. |
Thesaurus Nal: |
Biofilm; Disease resistance; Mastitis; Sheep diseases; Toxins. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02716naa a2200325 a 4500 001 2072041 005 2017-11-21 008 2017 bl uuuu u00u1 u #d 022 $a0022-0302 024 7 $ahttps://doi.org/10.3168/jds.2016-11583$2DOI 100 1 $aMARTINS, K. B. 245 $aCharacteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis.$h[electronic resource] 260 $c2017 520 $aCoagulase-negative staphylococci (CNS) are among the main responsible agents for mastitis in sheep. Cure rates can be reduced due to several causes, such as those related to virulence factors presented by microorganisms. This study aims at characterizing the virulence and resistance factors to antimicrobial agents in different CNS species isolated from sheep milk. After collecting milk samples, the samples were analyzed and the CNS species were identified. After identification, the susceptibility-sensitivity profile was examined using the disk diffusion technique for 10 antimicrobial agents. The DNA was extracted to detect the presence of the mecA gene, biofilm (icaADBC, bap, and bhp) and toxin genes (sea, seb, sec, sed, tst, and luk-PV) by PCR. Samples carrying toxin genes had their expression assessed using the reverse-transcription PCR technique. The biofilm production was assessed using the adherence method on a polystyrene plate. One hundred twelve CNS samples were isolated, 53 (47.3%) from animals with subclinical mastitis and 59 (52.7%) from healthy animals. Drugs tested have shown to be efficient for most CNS samples. The largest resistance percentage of CNS was found for the penicillin (17.0%) and tetracycline (10.7%) and 4 samples carried the mecA gene. As for the biofilm genes, the icaADBC operon was found in 10 (8.9%) samples, the bap gene was found in 16 (14.3%), and the bhp gene was found in 3 (2.7%). In addition, 69 (61.6%) samples produced biofilm. The survey of toxin genes has shown that 70 (62.5%) samples showed some toxin-encoding gene. However, none of the samples has expressed any of the genes from those toxins studied. 650 $aBiofilm 650 $aDisease resistance 650 $aMastitis 650 $aSheep diseases 650 $aToxins 653 $aCoagulase-negative staphylococci 653 $aResistance 653 $aSheep mastitis 700 1 $aFACCIOLI, P. Y. 700 1 $aBONESSO, M. F. 700 1 $aFERNANDES, S. 700 1 $aOLIVEIRA, A. A. 700 1 $aDANTAS, A. 700 1 $aZAFALON, L. F. 700 1 $aCUNHA, M. de L. R. de S. da 773 $tJournal of Dairy Science$gv. 100, n. 3, p. 2184-2195, 2017.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
19/03/2009 |
Data da última atualização: |
16/02/2024 |
Tipo da produção científica: |
Documentos |
Autoria: |
SILVA, J. N. M.; CARVALHO, J. O. P. de; MENEZES, N. P. de; SILVA, U. S. da C.; MARTINS FILHO, S. E. C.; TAVARES, M. J. M.; LELIS, E. A. |
Afiliação: |
JOSÉ NATALINO MACEDO SILVA, SERVIÇO FLORESTAL BRASILEIRO; JOAO OLEGARIO PEREIRA DE CARVALHO, CPATU; NAIANA PERES DE MENEZES, IBAMA; ULISSES SIDNEI DA CONCEIÇÃO SILVA; SERGIO EVANDRO COSTA MARTINS FILHO; MÁRIO JOSÉ MATOS TAVARES, AMASOFT; EVANDRO AMORIM LELIS, AMASOFT. |
Título: |
Ferramenta para cálculo de volumes de smalian: manual do usuário. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Belém, PA: Embrapa Amazônia Oriental, 2008. |
Páginas: |
41 p. |
Descrição Física: |
il. |
Série: |
(Embrapa Amazônia Oriental. Documentos, 317). |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Manejo florestal; Software. |
Thesagro: |
Análise de Dados; Árvore; Floresta Tropical; Produção Florestal. |
Thesaurus NAL: |
Amazonia. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/27995/1/Doc317.pdf
|
Marc: |
LEADER 00834nam a2200289 a 4500 001 1410131 005 2024-02-16 008 2008 bl uuuu u0uu1 u #d 100 1 $aSILVA, J. N. M. 245 $aFerramenta para cálculo de volumes de smalian$bmanual do usuário. 260 $aBelém, PA: Embrapa Amazônia Oriental$c2008 300 $a41 p.$cil. 490 $a(Embrapa Amazônia Oriental. Documentos, 317). 650 $aAmazonia 650 $aAnálise de Dados 650 $aÁrvore 650 $aFloresta Tropical 650 $aProdução Florestal 653 $aBrasil 653 $aManejo florestal 653 $aSoftware 700 1 $aCARVALHO, J. O. P. de 700 1 $aMENEZES, N. P. de 700 1 $aSILVA, U. S. da C. 700 1 $aMARTINS FILHO, S. E. C. 700 1 $aTAVARES, M. J. M. 700 1 $aLELIS, E. A.
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