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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2017 |
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Data da última atualização: |
14/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, S. O. D. de; SAYD, R. M.; BALZON, T. A.; SCHERWINSKI PEREIRA, J. E. |
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Afiliação: |
Sharrine Omari Domingues de Oliveira, Universidade Federal de Viçosa; Ricardo Meneses Sayd, UnB; Talita Aparecida Balzon, Universidade Federal do Amazonas; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
A new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Scientia Horticulturae, v. 161, p. 204-209, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
We have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results indicate the establishment of a new procedure for vanilla micropropagation through DPS, with a high production of plantlets at the end of the process and great potential for agriculture application. In addition, the presented system is more economic than the single-phase during the subcultures. MenosWe have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results ind... Mostrar Tudo |
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Palavras-Chave: |
Shoot proliferation. |
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Thesagro: |
Micropropagação. |
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Thesaurus Nal: |
micropropagation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02408naa a2200193 a 4500
001 2067485
005 2024-06-14
008 2013 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, S. O. D. de
245 $aA new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system.$h[electronic resource]
260 $c2013
520 $aWe have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results indicate the establishment of a new procedure for vanilla micropropagation through DPS, with a high production of plantlets at the end of the process and great potential for agriculture application. In addition, the presented system is more economic than the single-phase during the subcultures.
650 $amicropropagation
650 $aMicropropagação
653 $aShoot proliferation
700 1 $aSAYD, R. M.
700 1 $aBALZON, T. A.
700 1 $aSCHERWINSKI PEREIRA, J. E.
773 $tScientia Horticulturae$gv. 161, p. 204-209, 2013.
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