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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
14/03/2017 |
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Data da última atualização: |
14/03/2017 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
COTTA, M. G. |
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Afiliação: |
MICHELLE GUITTON COTTA. |
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Título: |
Molecular mechanisms in the first step of aba-mediated response in Coffea ssp. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
2016. |
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Páginas: |
169 p. |
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Idioma: |
Inglês |
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Notas: |
Tese (doutorado Biotecnologia Vegetal) - Universidade Federal de Lavras, Lavras, MG. Orientador: Alan Carvalho Andrade. Coorientador: Pierre Roger René Marraccini. |
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Conteúdo: |
Abscisic acid (ABA) pathway is a phytohormone universally conserved in land plants which coordinates several aspects of the plant response to water deficit such as root architecture, seed dormancy and regulation of stomatal closure. A mechanism of ABA signal transduction has been proposed, evolving intracellular ABA receptors (PYR/PYL/RCARs) interacting with PP2Cs phosphatases and SnRK2 protein kinases regulating this tripartite protein system. The goal of this study was to identify and characterize for the first time the orthologs genes of this tripartite system in Coffea. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, tomato and potato were chosen as query to search orthologous genes in the Coffee Genome Hub (http://coffee-genome.org/). Differential expression in tissues as leaves, seeds, roots and floral organs waschecked through in silico analyses. In vivo gene expression analyses were also performed by RT-qPCR in leaves and roots of drought-tolerant (DT 14, 73 and 120) and drought-susceptible (DS 22) C. canephora Conilon clones submitted (or not) to drought. The expression profiles of the tripartite system CcPYL-PP2C-SnRK2 genes were also analyzed in leaves of C. arabica and C. canephora plants growing under hydroponic condition and submitted to ABA exogenous treatment (500 ?M). This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCARs, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein motifs identified in predict coffee sequences enabled characterize these genes as family’s members of PYL/RCARs receptors, PP2Cs phosphatases or SnRK2 kinases of the ABA response pathway. These families were functionally annotated in the C. canephora genome. In vivo analyses revealed that eight genes are upregulated under drought conditions in both leaves and roots tissues. Among them, three genes coding phosphatases were expressed in all (DT and DS) clones therefore suggesting that they were activated as a general response to cope with drought stress. However, two other phosphatase coding genes were up-regulated only in the DT clones, suggesting that they constituted key-genes for drought tolerance in these clones. The DT clones also showed differential gene expression profiles for five other genes therefore reinforcing the idea that multiple biological mechanisms are involved drought tolerance in C. canephora. In response to exogenous ABA, 17 genes were expressed in leaves of C. canephora and C. arabica plants. Several genes were differentially expressed in the DT clone 14 either in control condition or after 24h ABA treatment. Under control conditions, five genes were higher expressed in the C. canephora and C. arabica DT plants. The kinase CcSnRK2.6 was highlighted as gene specifically expressed in the C. canephora plants (DT and DS) after 72h of ABA treatment. Overall, it was observed that ABA signaling pathway is delayed in the DS C. arabica Rubi. Those molecular evidences corroborated with microscopies analyses which showed that the DT clone 14 was more efficient to control stomatal closure than other coffee plants in response to ABA treatment. All these evidences will help us to identify the genetic determinism of drought tolerance through ABA pathway essential to obtain molecular markers that could be used in coffee breeding programs.. MenosAbscisic acid (ABA) pathway is a phytohormone universally conserved in land plants which coordinates several aspects of the plant response to water deficit such as root architecture, seed dormancy and regulation of stomatal closure. A mechanism of ABA signal transduction has been proposed, evolving intracellular ABA receptors (PYR/PYL/RCARs) interacting with PP2Cs phosphatases and SnRK2 protein kinases regulating this tripartite protein system. The goal of this study was to identify and characterize for the first time the orthologs genes of this tripartite system in Coffea. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, tomato and potato were chosen as query to search orthologous genes in the Coffee Genome Hub (http://coffee-genome.org/). Differential expression in tissues as leaves, seeds, roots and floral organs waschecked through in silico analyses. In vivo gene expression analyses were also performed by RT-qPCR in leaves and roots of drought-tolerant (DT 14, 73 and 120) and drought-susceptible (DS 22) C. canephora Conilon clones submitted (or not) to drought. The expression profiles of the tripartite system CcPYL-PP2C-SnRK2 genes were also analyzed in leaves of C. arabica and C. canephora plants growing under hydroponic condition and submitted to ABA exogenous treatment (500 ?M). This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCARs, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein motifs ide... Mostrar Tudo |
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Palavras-Chave: |
Déficit hídrico. |
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Thesagro: |
Ácido abscísico; Hormonio vegetal. |
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Thesaurus Nal: |
Abscisic acid; Coffea; Plant hormones. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04070nam a2200205 a 4500
001 2066980
005 2017-03-14
008 2016 bl uuuu m 00u1 u #d
100 1 $aCOTTA, M. G.
245 $aMolecular mechanisms in the first step of aba-mediated response in Coffea ssp.$h[electronic resource]
260 $a2016.$c2016
300 $a169 p.
500 $aTese (doutorado Biotecnologia Vegetal) - Universidade Federal de Lavras, Lavras, MG. Orientador: Alan Carvalho Andrade. Coorientador: Pierre Roger René Marraccini.
520 $aAbscisic acid (ABA) pathway is a phytohormone universally conserved in land plants which coordinates several aspects of the plant response to water deficit such as root architecture, seed dormancy and regulation of stomatal closure. A mechanism of ABA signal transduction has been proposed, evolving intracellular ABA receptors (PYR/PYL/RCARs) interacting with PP2Cs phosphatases and SnRK2 protein kinases regulating this tripartite protein system. The goal of this study was to identify and characterize for the first time the orthologs genes of this tripartite system in Coffea. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, tomato and potato were chosen as query to search orthologous genes in the Coffee Genome Hub (http://coffee-genome.org/). Differential expression in tissues as leaves, seeds, roots and floral organs waschecked through in silico analyses. In vivo gene expression analyses were also performed by RT-qPCR in leaves and roots of drought-tolerant (DT 14, 73 and 120) and drought-susceptible (DS 22) C. canephora Conilon clones submitted (or not) to drought. The expression profiles of the tripartite system CcPYL-PP2C-SnRK2 genes were also analyzed in leaves of C. arabica and C. canephora plants growing under hydroponic condition and submitted to ABA exogenous treatment (500 ?M). This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCARs, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein motifs identified in predict coffee sequences enabled characterize these genes as family’s members of PYL/RCARs receptors, PP2Cs phosphatases or SnRK2 kinases of the ABA response pathway. These families were functionally annotated in the C. canephora genome. In vivo analyses revealed that eight genes are upregulated under drought conditions in both leaves and roots tissues. Among them, three genes coding phosphatases were expressed in all (DT and DS) clones therefore suggesting that they were activated as a general response to cope with drought stress. However, two other phosphatase coding genes were up-regulated only in the DT clones, suggesting that they constituted key-genes for drought tolerance in these clones. The DT clones also showed differential gene expression profiles for five other genes therefore reinforcing the idea that multiple biological mechanisms are involved drought tolerance in C. canephora. In response to exogenous ABA, 17 genes were expressed in leaves of C. canephora and C. arabica plants. Several genes were differentially expressed in the DT clone 14 either in control condition or after 24h ABA treatment. Under control conditions, five genes were higher expressed in the C. canephora and C. arabica DT plants. The kinase CcSnRK2.6 was highlighted as gene specifically expressed in the C. canephora plants (DT and DS) after 72h of ABA treatment. Overall, it was observed that ABA signaling pathway is delayed in the DS C. arabica Rubi. Those molecular evidences corroborated with microscopies analyses which showed that the DT clone 14 was more efficient to control stomatal closure than other coffee plants in response to ABA treatment. All these evidences will help us to identify the genetic determinism of drought tolerance through ABA pathway essential to obtain molecular markers that could be used in coffee breeding programs..
650 $aAbscisic acid
650 $aCoffea
650 $aPlant hormones
650 $aÁcido abscísico
650 $aHormonio vegetal
653 $aDéficit hídrico
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Embrapa Café (CNPCa) |
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| Registros recuperados : 20 | |
| 3. |  | MAIA, S. M. F.; XAVIER, F. A. S.; OLIVEIRA, T. S.; MENDONÇA, E. S.; ARAUJO FILHO, J. A. de. Compartimentos da matéria orgânica em sistemas agroflorestais e convencinal no trópico semi-árido cearense. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 30., 2005, Recife. Solos, sustentabilidade e qualidade ambiental: [ palestras e trabalhos]. Recife: Sociedade Brasileira de Ciência do Solo, 2005. 4 f. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|    |
| 6. | | MAIA, S. M. F.; XAVIER, F. A. S.; OLIVEIRA, T. S.; MENDONÇA, E. S.; ARAUJO FILHO, J. A. de. Organic carbon pools in a luvisol under agroforestry and conventional farming systems in the semi-arid region of Ceará, Brazil. Agroforestry Systems, v. 71, n. 2, p. 127-138, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|  |
| 7. | | AGUIAR, M. I. de; MAIA, S. M. F.; OLIVEIRA, T. S. de; MENDONÇA, E. S.; ARAÚJO FILHO, J. A. de. Perdas de solo, água e nutrientes em sistemas agroflorestais no município de Sobral, CE. Revista Ciência Agronômica, v. 37, n. 3, p.270-278, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | MAIA, S. M. F.; MEDEIROS, A. de S.; SANTOS, T. C. dos; LYRA, G. B.; LAL, R.; ASSAD, E. D.; CERRI, C. E. P. Potential of no-till agriculture as a nature-based solution for climate-change mitigation in Brazil. Soil & Tillage Research, v. 220, 105368, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 10. | | AGUIAR, M. I. de; MAIA, S. M. F.; XAVIER, F. A. da S.; MENDONÇA, E. de S.; ARAUJO FILHO, J. A. de; OLIVEIRA, T. S. de. Sediment, nutrient and water losses by water erosion under agroforestry systems in the semi-arid region in northeastern Brazil. Agroforest System, v. 79, n. 3, p. 277-289, Jul. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | MAIA, S. M. F.; XAVIER, F. A. da S.; OLIVEIRA, T. S. de; MENDONÇA, E. de S.; ARAUJO FILHO, J. A. de. Impactos de sistemas agroflorestais e convencional sobre a qualidade do solo no semi-árido cearense. Revista Árvore, Viçosa, MG, v. 30, n. 5, p. 837-848, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | MAIA, S. M. P. C.; PONTES, D. F.; GARRUTI, D. dos S.; OLIVEIRA, M. N. DE; ARCANJO, S. R. S.; CHINELATE, G. C. B. Farinha de maracujá na elaboração de bolo de milho. Revista Verde de Agroecologia e Desenvolvimento Sustentável, Pombal, v. 13, n. 3, p. 328-336, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 13. | | XAVIER, F. A. da S.; MAIA, S. M. F.; RIBEIRO, K. A. RAÚJO; MENDONÇA, E. de S.; OLIVEIRA, T. S. de. Effect of cover plants on soil C and N dynamics in different soil management systems in dwarf cashew culture. Agriculture, Ecosystems and Environment, v. 165, 2013, p. 173 - 183, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | BRAY, A. W.; KIM, J. H.; SCHRUMPF, M.; PEACOCK, C.; BANWART, S.; SCHIPPER, L.; ANGERS, D.; CHIRINDA, N.; ZINN, Y. L.; ALBRECHT, A.; KUIKMAN, P.; JOUQUET, P.; DEMENOIS, J.; FARRELL, M.; SOUSSANA, J.-F.; KUHNERT, M.; MILNE, E.; FONTAINE, S.; TAGHIZADEH-TOOSI, A.; CERRI, C. E. P.; CORBEELS, M.; CARDINAEL, R.; CERVANTES, V. A.; OLESEN, J. E.; BATJES, N.; HEUVELINK, G.; MAIA, S. M. F.; KEESSTRA, S.; CLAESSEN, L.; MADARI, B. E.; VERCHOT, L.; NIE, W.; BRUNELLE, T.; MORAN, D.; FRANK, S.; BODLE, R.; FRELIH-LARSEN, A.; DOUGILL, A.; MONTANARELLA, L.; STRINGER, L.; CHENU, C.; HIEDERER, R.; SMITH, P.; ARIAS-NAVARRO, C. The science base of a strategic research agenda: executive summary. Wageningen: CIRCASA, 2019. 15 p. European Union's Horizon 2020 Research and Innovation Programme Grant Agreement No 774378. Coordination of International Research Cooperation on Soil Carbon Sequestration in Agriculture.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 20. | | FONTANA, A.; PIRES, A. M. M.; VENTURIERI, A.; JESUS, A. H. de; PAULA, A. J. de; MEDEIROS, A. de S.; VARGAS, A. de G.; BERNDT, A.; FERREIRA, A. L.; LUIZ, A. J. B.; ALVES, A. E.; PEREIRA, A. C.; FREITAS, A. F. de; PACKER, A. P.; CAVALCANTE, A. P. C. M.; FRASCA, A. V. da L.; FERNANDES, A.; ZIMBRES, B.; RUDORFF, B. F. T.; DENARDI, B. M.; BORDRON, B.; ALVES, B. J. R.; KRAHEMBUHL, C. B. B. P.; ARAÚJO, C. E. R.; DUBEUX, C. B. S; MAEQUES, C. L.; SILVA, C. R.; BALDI, C.; JANTALIA, C. P.; GESTEIRA NETO, C. J. M.; BACK, C. D.; ANDRADE, C. A. de; AGUIAR, D. A. de; OBERLING, D. F.; OLIVEIRA, D. M. de; CALÇADO, D. M.; GONÇALVES, D. N. S.; MOLLETA, D. G. S.; GAROFALO, D. F. T.; FERREIRA, D.; GOULART, D.; OLIVEIRA, E. C. de; ROSA, E. R.; ALMEIDA, E. G. de; ALBUQUERQUE, E. R. G. M. de; LA ROVERE, E. L.; RIEGELHAUPT, E. M.; HENRIQUE, F. L.; FARIA, F. F. de; PATERNOST, F. F.; CESÁRIO, F. V.; PEREIRA, F. R. de S.; PAREYN, F. G. C.; RODRIGUES, G. A. H.; BERTANI, G.; LEMOS, G. da S.; RIBEIRO, G. H. P. de M.; CABRAL, G. H.; FROES, G. A. M.; GOES, G. V.; ANDRADE, G. S. de; CARVALHO, G. C. de; NASPOLINI, G. F.; CHRISTO, G. L. de; SANTOS, G. V. dos; BARROS, H. H. D. de; HASENACK, H.; TAVARES, H. C.; MARTINS, I. T.; FRANÇA, I. E. R.; ROITMAN, I.; SOARES, I. M. de M.; SIMIONATO, J.; FREITAS, J.; OMETTO, J. P. H. B.; LINARES, J. A. H.; RISSO, J.; CRUZ FILHO, J. L. V. da; QUINTÃO, J. M. B.; SILVA, J. S. O.; LANA, J. T. de O.; ZANATTA, J. A.; MARACAHIPES, L.; RIBEIRO, L. da S.; VICENTE, L. E.; SCHRAMM, L. F. P.; ROSSI, L. M. B.; SILVA, M. G. da; SARAIVA, M.; D'AGOSTO, M. de A.; VIRTUOSO, M. A.; LIGO, M. A. V.; RACHWAL, M. F. G.; ROSA, M.; PICHARILLO, M. E.; SILVA, M. G. L. da; MATSUURA, M. I. da S. F.; DALLA BETTA, M. M.; PEREIRA, M. J.; CARON, M. L.; ASSIS, M. L. R. de; SANTOS, M. M. de O.; ROCHA, M. B.; BUSTAMANTE, M. M. da C.; WALTER, M. C.; AQUINO, M. F. S. de; SALGADO, M. P. G.; SERRUYA, N. M.; MOREIRA, N. de P.; FERREIRA, N. C.; RAMOS, N. P.; CABRAL, O. M. R.; MOSER, P.; COLTURATO, P. D.; GRÜTZMACHER, P.; AUGUSTO, R. C.; GRISOLI, R. P. S.; SOLARI, R.; PAZIANOTTO, R. A. A.; DUPONT, R. A. B.; CANTINHO, R. Z.; PEIXOTO, R. de A.; PADILHA, R. A.; NICOLOSO, R. da S.; OLIVEIRA, R. R. S. de; HIGA, R. C. V.; GUIDOTTI, R. M. M.; NOGUEIRA, S. F.; MAIA, S. M. F.; ASSIS, T. F. de; IGAWA, T. K.; RODRIGUES, T. da S.; CARMO, T. R. L. do; MIRANDA, V. S.; KONDO, V. Y.; SCIVITTARO, W. B.; WILLS, W.; HOLLER, W. A. Inventário nacional de emissões e remoções antrópicas de gases de efeito estufa. In: BRASIL. Ministério da Ciência, Tecnologia e Inovações. Quarta Comunicação Nacional do Brasil à Convenção-Quadro das Nações Unidas sobre Mudança do Clima. Brasília, DF, 2021. cap. 2, p. 80-181.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Amazônia Oriental; Embrapa Florestas; Embrapa Pecuária Sudeste; Embrapa Solos. |
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| Registros recuperados : 20 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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