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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NANI, T. F.; PEREIRA, D. L.; SOUZA SOBRINHO, F. de; TECHIO, V. H. |
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Afiliação: |
Thaís Furtado Nani, UFLA; Daniele Laís Pereira, UFLA; FAUSTO DE SOUZA SOBRINHO, CNPGL; Vânia Helena Techio, UFLA. |
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Título: |
Physical map of repetitive DNA sites in Brachiaria spp.: intravarietal and interspecific polymorphisms. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Crop Science, v. 56, n. 4, p. 1769-1783, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Brachiaria (Trin.) Griseb. is a genus belonging to the Poaceae Barnh. family that aggregates species of forage interest, which have shown different levels of ploidy, intraspecific variability for chromosomal morphology and for number of rDNA sites. This study aimed at characterizing the karyotype of Brachiaria brizantha (Hochst. ex A. Rich) Stapf, Brachiaria decumbens Stapf, and Brachiaria ruziziensis R. Germ. & C.M. Evrard by the location of 5S rDNA, 45S rDNA, centromeric retrotransposons, chromomycin A3 (CMA) and 4¢, 6-diamidino-2-phenylindole dihydrochloride (DAPI) chromosome bands, and transcriptional activity at nucleolar organizing regions (NORs). Brachiaria ruziziensis has 2n = 2x = 18 chromosomes, karyotypic formula 9M, one 45S rDNA site, and two 5S rDNA sites. Brachiaria brizantha has 2n = 4x = 36 chromosomes, karyotypic formula 18M, four to six 5S rDNA signals, and two to four 45S rDNA sites. One of the chromosomes carrying 45S rDNA is heteromorphic. Brachiaria decumbens has 2n = 4x = 36 chromosomes, karyotypic formula 17M+1M/SM, and seven 5S rDNA signals including one hemizygous 5S rDNA locus. One of the two chromosome pairs carrying 45S rDNA signals was heteromorphic with one of its chromosomes possessing a tertiary constriction. Hybridization signals with a centromeric retrotransposon probe, CRM1, were observed in Brachiaria chromosomes with distinct clusters on all B. ruziziensis chromosomes. Chromosomal CMA bands were located along with 45S rDNA signals in B. decumbens and B. ruziziensis whereas in B. brizantha only two out of four signals coincided. The karyotype of Brachiaria species is symmetrical. The transcriptional activity of NORs is variable with two or three heteromorphic nucleoli. MenosAbstract Brachiaria (Trin.) Griseb. is a genus belonging to the Poaceae Barnh. family that aggregates species of forage interest, which have shown different levels of ploidy, intraspecific variability for chromosomal morphology and for number of rDNA sites. This study aimed at characterizing the karyotype of Brachiaria brizantha (Hochst. ex A. Rich) Stapf, Brachiaria decumbens Stapf, and Brachiaria ruziziensis R. Germ. & C.M. Evrard by the location of 5S rDNA, 45S rDNA, centromeric retrotransposons, chromomycin A3 (CMA) and 4¢, 6-diamidino-2-phenylindole dihydrochloride (DAPI) chromosome bands, and transcriptional activity at nucleolar organizing regions (NORs). Brachiaria ruziziensis has 2n = 2x = 18 chromosomes, karyotypic formula 9M, one 45S rDNA site, and two 5S rDNA sites. Brachiaria brizantha has 2n = 4x = 36 chromosomes, karyotypic formula 18M, four to six 5S rDNA signals, and two to four 45S rDNA sites. One of the chromosomes carrying 45S rDNA is heteromorphic. Brachiaria decumbens has 2n = 4x = 36 chromosomes, karyotypic formula 17M+1M/SM, and seven 5S rDNA signals including one hemizygous 5S rDNA locus. One of the two chromosome pairs carrying 45S rDNA signals was heteromorphic with one of its chromosomes possessing a tertiary constriction. Hybridization signals with a centromeric retrotransposon probe, CRM1, were observed in Brachiaria chromosomes with distinct clusters on all B. ruziziensis chromosomes. Chromosomal CMA bands were located along with 45S rDNA signa... Mostrar Tudo |
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Palavras-Chave: |
CRM1; Cytogenetic analyses; Intravarietal variations; Karyotypes of Brachiaria spp. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| 2. |  | ALENCAR, M. M. de. Seleção no gado Canchim. Palestra apresentada na 1ª convenção de criadores técnicos e jurados da raça Canchim, Presidente Prudente, SP, 15/09/93. São Paulo: ABCCAN, 1993. 32 p.| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | ALENCAR, M. M. de. The Canchim beef cattle breed. In: GLOBAL CONFERENCE ON CONSERVATION OF DOMESTIC ANIMAL GENETICO, RESOURCES, 15., 2000, Brasilia, DF. Conservation and Biotecnology: a balanced approach for the new millenirium. Proceedings... Brasilia: Embrapagenetic resources and Biotechonology, 2000. 5 p. Resumo| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | ALENCAR, M. M. de. Avaliação do gado Canchim e de seus cruzamentos. In: PUIGNAU, J. P. (Ed.). Evaluación y elección de biotipos de acuerdo a los sistemas de producción. Montevideo (Uruguay): IICA/PROCISUR, 1993. p.303-316. (Dialogo IICA-PROCISUR, 35).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | ALENCAR, M. M. de. Rebanho de fino trato. In: SABERES acumulados: resultados de projetos temáticos em São Paulo. São Paulo: FAPESP, 2007. 216p. il. (Jornalismo científico, v. 8). p. 23-30.| Tipo: Capítulo em Livro Técnico-Científico |
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