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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/12/2016 |
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Data da última atualização: |
07/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MIRANDA, Í. K. S. P. B.; MIRANDA, A. F. S.; SOUZA, F. V. D.; PIROVANI, C. P.; PEPE, I. M.; RODOWANSKI, I. J.; FERREIRA, K. T. de S. E.; VAZ, L. M. S.; ASSIS, S. A. de. |
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Afiliação: |
ÍNGARA KEISLE SÃO PAULO BARRETTO MIRANDA; ANDERSON FONTES SUZART MIRANDA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; CARLOS PRIMINHO PIROVANI; IURI MUNIZ PEPE; IVANOÉ JOÃO RODOWANSKI; KATIÚCIA TÍCILA DE SOUZA EDUVIRGENS FERREIRA; LUCIANO MENDES SOUZA VAZ; SANDRA APARECIDA DE ASSIS. |
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Título: |
The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
International Journal of Food Sciences and Nutrition, 2016 |
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ISSN: |
0963-7486 |
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Idioma: |
Português |
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Conteúdo: |
The current study aims to extract bromelain from different parts (stem, crown, peels, pulp and leaves) of Ananas comosus var. comosus AGB 772; to determine of optimum pH and temperature; to test bromelain stability in disodium EDTA and sodium benzoate, and to investigate its pharmacological activity on B16F10 murine melanoma cells in vitro. The highest enzymatic activity was found in bromelain extracted from the pulp and peel. The optimum bromelain pH among all studied pineapple parts was 6.0. The optimum temperature was above 50?°C in all bromelain extracts. The fluorescence analysis confirmed the stability of bromelain in the presence of EDTA and sodium benzoate. Bromelain was pharmacologically active against B16F10 melanoma cells and it was possible verifying approximately 100% inhibition of tumor cell proliferation in vitro. Since bromelain activity was found in different parts of pineapple plants, pineapple residues from the food industry may be used for bromelain extraction. |
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Thesagro: |
Bromélia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01766naa a2200241 a 4500
001 2059254
005 2017-02-07
008 2016 bl uuuu u00u1 u #d
022 $a0963-7486
100 1 $aMIRANDA, Í. K. S. P. B.
245 $aThe biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells.$h[electronic resource]
260 $c2016
520 $aThe current study aims to extract bromelain from different parts (stem, crown, peels, pulp and leaves) of Ananas comosus var. comosus AGB 772; to determine of optimum pH and temperature; to test bromelain stability in disodium EDTA and sodium benzoate, and to investigate its pharmacological activity on B16F10 murine melanoma cells in vitro. The highest enzymatic activity was found in bromelain extracted from the pulp and peel. The optimum bromelain pH among all studied pineapple parts was 6.0. The optimum temperature was above 50?°C in all bromelain extracts. The fluorescence analysis confirmed the stability of bromelain in the presence of EDTA and sodium benzoate. Bromelain was pharmacologically active against B16F10 melanoma cells and it was possible verifying approximately 100% inhibition of tumor cell proliferation in vitro. Since bromelain activity was found in different parts of pineapple plants, pineapple residues from the food industry may be used for bromelain extraction.
650 $aBromélia
700 1 $aMIRANDA, A. F. S.
700 1 $aSOUZA, F. V. D.
700 1 $aPIROVANI, C. P.
700 1 $aPEPE, I. M.
700 1 $aRODOWANSKI, I. J.
700 1 $aFERREIRA, K. T. de S. E.
700 1 $aVAZ, L. M. S.
700 1 $aASSIS, S. A. de
773 $tInternational Journal of Food Sciences and Nutrition, 2016
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| 1. |  | OLIVEIRA, E. J. de; OLIVEIRA, S. A. S. de; OTTO, C.; ALICAI, T.; FREITAS, J. P. X. de; CORTES, D. F. M.; PARIYO, A.; LIRI, C.; ADIGA, G.; BALMER, A.; ROBINSON, M. A novel seed treatment-based multiplication approach for cassava planting material. Plos One, v.15, n.3, e0229943, March, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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