|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
15/12/2016 |
|
Data da última atualização: |
27/06/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PINHEIRO, P. V.; GHANIM, M.; ALEXANDER, M.; REBELO, A. R.; SANTOS, R. S.; ORSBURN, B. C.; GRAY, S.; CILIA, M. |
|
Afiliação: |
PATRICIA VALLE PINHEIRO, CNPAF; MURAD GHANIM; MARIKO ALEXANDER; ANA RITA REBELO; ROGERIO S. SANTOS; BENJAMIN C. ORSBURN; STEWART GRAY; MICHELLE CILIA. |
|
Título: |
Host plants indirectly influence plant virus transmission by altering gut cysteine protease activity of aphid vectors. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Molecular & Cellular Proteomics, v. 16, n. 4 suppl. 1, p. S230-S243, Apr. 2017. |
|
DOI: |
10.1074/mcp.M116.063495 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The green peach aphid, Myzus persicae, is a vector of the Potato leafroll virus (PLRV, Luteoviridae), transmitted exclusively by aphids in a circulative manner. PLRV transmission efficiency was significantly reduced when a clonal lineage of M. persicae was reared on turnip as compared to the weed physalis, a transient effect caused by a host-switch response. A trend of higher PLRV titer in physalis-reared aphids as compared to turnip-reared aphids was observed at 24h and 72h after virus acquisition. The major difference in the proteomes of these aphids was the upregulation of predicted lysosomal enzymes, in particular the cysteine protease cathepsin B (cathB), in aphids reared on turnip. The aphid midgut is the site of PLRV acquisition, and cathB and PLRV localization were starkly different in midguts of the aphids reared on the two host plants. In viruliferous aphids that were reared on turnip, there is near complete co-localization of cathB and PLRV at the cell membranes, which was not observed in physalis-reared aphids. Chemical inhibition of cathB restored the ability of aphids reared on turnip to transmit PLRV in a dose-dependent manner, showing that the increased activity of cathB and other cysteine proteases at the cell membrane indirectly decreases virus transmission by aphids. Understanding how the host plant influences virus transmission by aphids is critical for growers to manage the spread of virus among field crops. |
|
Palavras-Chave: |
Host resistance. |
|
Thesagro: |
Praga de planta; Vírus. |
|
Thesaurus Nal: |
Aphidoidea; Plant viruses; Potato leafroll virus; Virus transmission. |
|
Categoria do assunto: |
O Insetos e Entomologia |
|
Marc: |
LEADER 02359naa a2200301 a 4500
001 2058750
005 2017-06-27
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1074/mcp.M116.063495$2DOI
100 1 $aPINHEIRO, P. V.
245 $aHost plants indirectly influence plant virus transmission by altering gut cysteine protease activity of aphid vectors.$h[electronic resource]
260 $c2017
520 $aThe green peach aphid, Myzus persicae, is a vector of the Potato leafroll virus (PLRV, Luteoviridae), transmitted exclusively by aphids in a circulative manner. PLRV transmission efficiency was significantly reduced when a clonal lineage of M. persicae was reared on turnip as compared to the weed physalis, a transient effect caused by a host-switch response. A trend of higher PLRV titer in physalis-reared aphids as compared to turnip-reared aphids was observed at 24h and 72h after virus acquisition. The major difference in the proteomes of these aphids was the upregulation of predicted lysosomal enzymes, in particular the cysteine protease cathepsin B (cathB), in aphids reared on turnip. The aphid midgut is the site of PLRV acquisition, and cathB and PLRV localization were starkly different in midguts of the aphids reared on the two host plants. In viruliferous aphids that were reared on turnip, there is near complete co-localization of cathB and PLRV at the cell membranes, which was not observed in physalis-reared aphids. Chemical inhibition of cathB restored the ability of aphids reared on turnip to transmit PLRV in a dose-dependent manner, showing that the increased activity of cathB and other cysteine proteases at the cell membrane indirectly decreases virus transmission by aphids. Understanding how the host plant influences virus transmission by aphids is critical for growers to manage the spread of virus among field crops.
650 $aAphidoidea
650 $aPlant viruses
650 $aPotato leafroll virus
650 $aVirus transmission
650 $aPraga de planta
650 $aVírus
653 $aHost resistance
700 1 $aGHANIM, M.
700 1 $aALEXANDER, M.
700 1 $aREBELO, A. R.
700 1 $aSANTOS, R. S.
700 1 $aORSBURN, B. C.
700 1 $aGRAY, S.
700 1 $aCILIA, M.
773 $tMolecular & Cellular Proteomics$gv. 16, n. 4 suppl. 1, p. S230-S243, Apr. 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
