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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
24/02/2016 |
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Data da última atualização: |
16/01/2026 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
SANCHES, M. M.; FERNANDES, F. R. |
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Afiliação: |
MARCIO MARTINELLO SANCHES, CENARGEN; FERNANDA RAUSCH FERNANDES, SIQ. |
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Título: |
Diagnostic methods for seed-transmitted viruses. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
GORAWALA, P.; MANDHATRI, S. (Ed.). Agricultural research updates. New York: Nova Science Publishers, 2015. v. 9, p. 41-62. |
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Idioma: |
Inglês |
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Conteúdo: |
It is estimated that more than 230 plant viruses are seed-transmitted in different food, fiber and ornamental crops, even as weeds. Despite a limited number of virus species that are transmitted in this manner, this is a primary factor for virus spread, especially through new areas. The transmission rate is variable between species, strains and plant hosts of viruses. There are two manners of seed infection by viruses. Infection of the embryo is the most common and external seed contamination. Only few virus species cause symptoms in seeds. Also, seeds are rich in compounds that are inhibitory for a number of laboratory tests. To overcome the difficulty to detect viruses, some different approaches have been used for diagnosis, like planting in greenhouse and further analysis: electron microscopy, ELISA test, PCR–based detection and more recently Real-Time PCR and NGS. The best diagnostic procedure to be used depends on the purpose of seed utilization and the seed transmission rates. However, the composition and the size of the seed could be limiting for some tests. In this chapter, the advantages of each strategy for diagnosis are discussed. |
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Palavras-Chave: |
Biological methods; ELISA test; PCR-based detection; Seed contamination; Seed infection. |
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Thesagro: |
Vírus. |
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Thesaurus Nal: |
Electron microscopy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01836naa a2200217 a 4500
001 2053734
005 2026-01-16
008 2015 bl uuuu u00u1 u #d
100 1 $aSANCHES, M. M.
245 $aDiagnostic methods for seed-transmitted viruses.$h[electronic resource]
260 $c2015
520 $aIt is estimated that more than 230 plant viruses are seed-transmitted in different food, fiber and ornamental crops, even as weeds. Despite a limited number of virus species that are transmitted in this manner, this is a primary factor for virus spread, especially through new areas. The transmission rate is variable between species, strains and plant hosts of viruses. There are two manners of seed infection by viruses. Infection of the embryo is the most common and external seed contamination. Only few virus species cause symptoms in seeds. Also, seeds are rich in compounds that are inhibitory for a number of laboratory tests. To overcome the difficulty to detect viruses, some different approaches have been used for diagnosis, like planting in greenhouse and further analysis: electron microscopy, ELISA test, PCR–based detection and more recently Real-Time PCR and NGS. The best diagnostic procedure to be used depends on the purpose of seed utilization and the seed transmission rates. However, the composition and the size of the seed could be limiting for some tests. In this chapter, the advantages of each strategy for diagnosis are discussed.
650 $aElectron microscopy
650 $aVírus
653 $aBiological methods
653 $aELISA test
653 $aPCR-based detection
653 $aSeed contamination
653 $aSeed infection
700 1 $aFERNANDES, F. R.
773 $tGORAWALA, P.; MANDHATRI, S. (Ed.). Agricultural research updates. New York: Nova Science Publishers, 2015.$gv. 9, p. 41-62.
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