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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
23/03/2016 |
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Data da última atualização: |
23/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, F. A. C.; MELO FILHO, P. DE A.; LIMA, L. M. de; SANTOS, R. C. dos. |
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Afiliação: |
FABIANA APARECIDA CAVALCANTE SILVA, UNIVERSIDADE DE SANTA CRUZ - ILHÉUS, BA; PÉRICLES DE ALBUQUERQUE MELO FILHO, UFRPE; LIZIANE MARIA DE LIMA, CNPA; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
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Título: |
A chlorophyllase differentially expressed in resistant cotton during infection with Colletotrichum gossypii var. cephalosporioides. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Asian Journal of Microbiology, Biotechnology & Environmental Sciences, v. 17, n. 4, p. 901-906, 2015. |
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ISSN: |
0972-3005 |
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Idioma: |
Inglês |
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Conteúdo: |
Resistant and susceptible cotton cultivars were infected with Colletotrichum gossypii var. Cephalosporioides in order to identify differentially expressed transcripts associated to disease tolerance. Plants were grown in greenhouse and sprayed with a fungus suspension at 1 x 106 conidia mL-1. Young leaves were collected to total RNA extraction at 3 and 15 days after inoculation. For molecular procedures, 3 days after inoculation leaves-RNA were used to RT-PCR reactions, with ten pre-selected RAPD primers. Eight of these primers exhibited polymorphic bands and were selected for transcripts analysis. Differential expression was visualized in both cultivars in down and up regulations and also new apparently activated transcripts were registered. A transcript (200 bp) obtained from cv. resistant BRS Antares exhibited homology (86%) with the GmChl 3 gene (AB181949.1) that codifies to Chlorophyllase III in soybean plants. Dot blot analysis revealed that this transcript was over-expressed just in a resistant cv. Antares at 3 and 15 days after inoculation. |
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Palavras-Chave: |
Cephalosporioides; Disease; Ramulosis. |
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Thesagro: |
Algodão; Colletotrichum gossypii; Stress. |
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Thesaurus Nal: |
cotton; fungi. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Algodão (CNPA) |
