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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
24/02/2016 |
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Data da última atualização: |
08/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOPES, T. A.; COSTA, J. N.; RIBEIRO, R. P.; PASSOS, J. R. S.; SOARES, M. A. A.; ALVES FILHO, J. G.; CUNHA, E. V.; HURK, R. van den; ANDRIOLI, A.; SILVA, J. R. V. |
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Afiliação: |
BIOTECHNOLOGY NUCLEUS OF SOBRAL, NUBIS, FEDERAL UNIVERSITY OF CEARA; BIOTECHNOLOGY NUCLEUS OF SOBRAL, NUBIS, FEDERAL UNIVERSITY OF CEARA; DEPARTMENT OF PATHOBIOLOGY, FACULTY OF VETERINARY MEDICINE, UTRECHT UNIVERSITY - UTRECHT, THE NETHERLANDS; ALICE ANDRIOLI PINHEIRO, CNPC. |
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Título: |
Influence of caprine arthritis encephalitis on expression of ovulation related genes and activation of primordial follicles cultured in presence of phytohemagglutinin, epidermal growth factor or both. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Small Ruminant Research, Amsterdam, v. 123, n. 2/3, p. 278-286, Feb. 2015. |
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DOI: |
10.1016/j.smallrumres.2014.11.016 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study investigated the levels of mRNA for BMP15, GDF9 and BMPR1B in preantral follicles, as well as in cumulus oocyte complex (COC) and follicular walls from antral follicles of healthy and CAEV infected goats by real-time PCR. In addition, the influence of phytohemagglutinin (PHA) and EGF on activation of primordial follicles and on expression of mRNA for TNFa and its receptors in cultured ovarian tissue from healthy and infected goats was evaluated. The expression of BMP15 and GDF9 in primordial/primary follicles from infected goats was significantly higher than in healthy animals, whereas the expression of BMP15 and GDF9 in secondary follicles from infected goats was significantly lower. The expression of mRNA for BMP15 in follicular wall of antral follicles from infected goats was significantly higher than in healthy goats. CAEV infection did not influence the ability of primordial follicles to develop in vitro, but the presence of EGF in culture medium significantly increased follicular diameter in healthy and infected animals. Ovarian tissue from infected goats cultured in medium with PHA had primordial follicles with larger diameters than those from healthy animals. An increase of mRNAs for TNF? was observed after culturing tissue in presence of both EGF and PHA in healthy animals, but this same treatment promoted a reduction of mRNAs for TNFa and increase of TNFR2 transcripts in infected animals. In conclusion, CAEV influences the expression of mRNA for BMP15 and GDF9 in goat ovarian follicles and PHA and EGF differentially regulate the expression of TNFa and TNFR2 in cultured ovarian tissue. MenosAbstract: This study investigated the levels of mRNA for BMP15, GDF9 and BMPR1B in preantral follicles, as well as in cumulus oocyte complex (COC) and follicular walls from antral follicles of healthy and CAEV infected goats by real-time PCR. In addition, the influence of phytohemagglutinin (PHA) and EGF on activation of primordial follicles and on expression of mRNA for TNFa and its receptors in cultured ovarian tissue from healthy and infected goats was evaluated. The expression of BMP15 and GDF9 in primordial/primary follicles from infected goats was significantly higher than in healthy animals, whereas the expression of BMP15 and GDF9 in secondary follicles from infected goats was significantly lower. The expression of mRNA for BMP15 in follicular wall of antral follicles from infected goats was significantly higher than in healthy goats. CAEV infection did not influence the ability of primordial follicles to develop in vitro, but the presence of EGF in culture medium significantly increased follicular diameter in healthy and infected animals. Ovarian tissue from infected goats cultured in medium with PHA had primordial follicles with larger diameters than those from healthy animals. An increase of mRNAs for TNF? was observed after culturing tissue in presence of both EGF and PHA in healthy animals, but this same treatment promoted a reduction of mRNAs for TNFa and increase of TNFR2 transcripts in infected animals. In conclusion, CAEV influences the expression of mRNA fo... Mostrar Tudo |
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Palavras-Chave: |
CAEV; Fitohemaglutinina; Ovary; Phytohaemagglutinins; TNF. |
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Thesagro: |
Cabra; Caprino; Doença Animal; Ovário. |
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Thesaurus Nal: |
Caprine arthritis encephalitis virus; Gene expression; Goats. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
null
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 3 | |
| 1. |  | OLEGÁRIO, R. M. B. N.; SILVA, V. P.; MACHADO, F. R. B.; AZEVEDO, V. C. R.; CIAMPI, A. Y. Análise da variabilidade genética da Dalbergia nigra (Jacarandá-da-Bahia) utilizando RAPD. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 57.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | AZEVEDO, V. C. R.; SILVA, V. P.; MACHADO, F. R. B.; OLEGÁRIO, R. M.; CIAMPI, A. Y. Análise genética de Tabebuia impetiginosa utilizando marcadores RAPD. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 60. Apresentado também no evento: CONGRESSO NACIONAL DE GENÉTICA, 48., 2002, Águas de Lindóia. A genética na inclusão social: anais. Águas de Lindóia: Sociedade Brasileira de Genética, 2002. Homenagem - ESALQ: Caminhos para o manejo de...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | VINSON, C. C.; AMARAL, A. C.; PROITE, K.; SILVA, V. da P.; OLEGÁRIO, R. M. B. N.; CIAMPI, A. Y.; SAMPAIO, I. Desenvolvimento de marcadores microssatélites para Jacaranda copaia (Leguminosae), uma espécie arbórea madeireira tropical. In: CONGRESSO NACIONAL DE GENÉTICA, 48., 2002, Águas de Lindóia. A genética na inclusão social: anais. Águas de Lindóia: Sociedade Brasileira de Genética, 2002. Homenagem - ESALQ: Caminhos para o manejo de recursos genéticos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 3 | |
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