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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
22/02/2016 |
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Data da última atualização: |
05/12/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIEIRA, D. D. S. S.; EMILIANIC, G.; MICHELOZZI, M.; CENTRITTO, M.; LUROE, F. S; MORILLON, R.; LORETO, F.; GESTEIRA, A. da S.; MASERTI, B. |
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Afiliação: |
DAYSE DRIELLY SOUZA SANTANA VIEIRA, CNR-IPSP; GIOVANNI EMILIANIC, CNR-IVALSA; MARCO MICHELOZZI, CNR-IBBR; MAURO CENTRITTO; FRANÇOIS LUROE; RAPHAËL MORILLON, UMR AGAP-INRA; FRANCESCO LORETO, NR-DISBA; ABELMON DA SILVA GESTEIRA, CNPMF; BIANCAELENA MASERTI, CNR-IPSP. |
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Título: |
Polyploidization alters constitutive content of volatile organic compounds (VOC) and improves membrane stability under water deficit in Volkamer lemon (Citrus limonia Osb.) leaves. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Environmental and Experimental Botany, n.126, p. 1-9, 2016. |
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ISSN: |
0098-8472 |
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Idioma: |
Inglês |
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Conteúdo: |
In Citrus species chromosome doubling naturally occurs in somatic embryos and doubled diploid plants often show better adaptation to adverse environmental condition. To understand the molecular determinants of stress acclimation, we examined the response to water deficit in diploid (2VL) and doubled diploid (4VL) seedlings of Volkamer lemon (Citrus limonia Osb.) assessing the profile of constitutive volatile organic compound (VOC) in control and stressed conditions. Physiological parameters and leaf volatile compound profiles were measured during water deficit and 24 h after rehydration of plants to field capacity. Net photosynthesis and stomatal conductance were reduced in water stressed leaves, with no significant differences between 2VL and 4VL plants. Malondialdehyde concentration, a marker of lipid peroxidation of cellular membranes, was significantly more higher in stressed 2VL leaves than in 4VL. The blend of constitutive VOC was different in control leaves being oxygenated sesquiterpenoids more abundant in 2VL leaves, and monoterpenoids more abundant in 4VL leaves. Water deficit did not stimulate biosynthesis of terpenoids, whereas accumulation of trans- 2 hexenal, a green leaf volatile (GLV) synthesized after membrane denaturation, was observed in stressed leaves of 2VL leaves, but not in 4VL leaves. Semiquantitative PCR showed an increase of the expression of HPL, the gene encoding for hydroperoxidase lyase which catalyzes 2-hexenal formation, only in 2VL plants. The expression of the putative dehydration transcription factor DREB2A was also observed only in 2VL water stressed plants. This work shows that level of ploidy may alter constitutive content of GLV by Citrus, therefore likely affecting plants capacity of protection and interaction with other organisms. Whereas diploid and double diploid plants showed similar physiological responses to water deficit, a biochemical marker indicated that membranes of double diploid leaves were more resistant to the stress.These results provide intriguing MenosIn Citrus species chromosome doubling naturally occurs in somatic embryos and doubled diploid plants often show better adaptation to adverse environmental condition. To understand the molecular determinants of stress acclimation, we examined the response to water deficit in diploid (2VL) and doubled diploid (4VL) seedlings of Volkamer lemon (Citrus limonia Osb.) assessing the profile of constitutive volatile organic compound (VOC) in control and stressed conditions. Physiological parameters and leaf volatile compound profiles were measured during water deficit and 24 h after rehydration of plants to field capacity. Net photosynthesis and stomatal conductance were reduced in water stressed leaves, with no significant differences between 2VL and 4VL plants. Malondialdehyde concentration, a marker of lipid peroxidation of cellular membranes, was significantly more higher in stressed 2VL leaves than in 4VL. The blend of constitutive VOC was different in control leaves being oxygenated sesquiterpenoids more abundant in 2VL leaves, and monoterpenoids more abundant in 4VL leaves. Water deficit did not stimulate biosynthesis of terpenoids, whereas accumulation of trans- 2 hexenal, a green leaf volatile (GLV) synthesized after membrane denaturation, was observed in stressed leaves of 2VL leaves, but not in 4VL leaves. Semiquantitative PCR showed an increase of the expression of HPL, the gene encoding for hydroperoxidase lyase which catalyzes 2-hexenal formation, only in 2VL plants. T... Mostrar Tudo |
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Thesagro: |
Fruta citríca. |
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Categoria do assunto: |
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Marc: |
LEADER 02826naa a2200241 a 4500
001 2037897
005 2016-12-05
008 2016 bl uuuu u00u1 u #d
022 $a0098-8472
100 1 $aVIEIRA, D. D. S. S.
245 $aPolyploidization alters constitutive content of volatile organic compounds (VOC) and improves membrane stability under water deficit in Volkamer lemon (Citrus limonia Osb.) leaves.$h[electronic resource]
260 $c2016
520 $aIn Citrus species chromosome doubling naturally occurs in somatic embryos and doubled diploid plants often show better adaptation to adverse environmental condition. To understand the molecular determinants of stress acclimation, we examined the response to water deficit in diploid (2VL) and doubled diploid (4VL) seedlings of Volkamer lemon (Citrus limonia Osb.) assessing the profile of constitutive volatile organic compound (VOC) in control and stressed conditions. Physiological parameters and leaf volatile compound profiles were measured during water deficit and 24 h after rehydration of plants to field capacity. Net photosynthesis and stomatal conductance were reduced in water stressed leaves, with no significant differences between 2VL and 4VL plants. Malondialdehyde concentration, a marker of lipid peroxidation of cellular membranes, was significantly more higher in stressed 2VL leaves than in 4VL. The blend of constitutive VOC was different in control leaves being oxygenated sesquiterpenoids more abundant in 2VL leaves, and monoterpenoids more abundant in 4VL leaves. Water deficit did not stimulate biosynthesis of terpenoids, whereas accumulation of trans- 2 hexenal, a green leaf volatile (GLV) synthesized after membrane denaturation, was observed in stressed leaves of 2VL leaves, but not in 4VL leaves. Semiquantitative PCR showed an increase of the expression of HPL, the gene encoding for hydroperoxidase lyase which catalyzes 2-hexenal formation, only in 2VL plants. The expression of the putative dehydration transcription factor DREB2A was also observed only in 2VL water stressed plants. This work shows that level of ploidy may alter constitutive content of GLV by Citrus, therefore likely affecting plants capacity of protection and interaction with other organisms. Whereas diploid and double diploid plants showed similar physiological responses to water deficit, a biochemical marker indicated that membranes of double diploid leaves were more resistant to the stress.These results provide intriguing
650 $aFruta citríca
700 1 $aEMILIANIC, G.
700 1 $aMICHELOZZI, M.
700 1 $aCENTRITTO, M.
700 1 $aLUROE, F. S
700 1 $aMORILLON, R.
700 1 $aLORETO, F.
700 1 $aGESTEIRA, A. da S.
700 1 $aMASERTI, B.
773 $tEnvironmental and Experimental Botany$gn.126, p. 1-9, 2016.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 32 | |
| 5. |  | POZZOBON, M. T.; PAGANELLA, M. B.; SANTOS, S. dos; VALLS, J. F. M. Cytological and reproductive aspects in the Caespitosa group of Paspalum. Ciência Rural, Santa Maria, v. 43, n. 11, p. 2004-2010, nov, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | MACHADO, A. C. de C.; POZZOBON, M. T.; VALLS, J. F. M.; SANTOS, S. dos; ARAUJO, F. L. Contagens cromossômicas em novos acessos de germoplasma de espécies de Paspalum (Gramineae) do Rio Grande do Sul do Brasil. In: WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4., 1999, Brasília. Anais: resumos dos trabalhos. Brasília, DF: EMBRAPA-CENARGEN, 1999. p. 78.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | MACHADO, A. C. C.; VALLS, J. F. M.; PEÑALOZA, A. del P. de S.; SANTOS, S, dos. Novos biótipos pentaplóides do grupo Dilatata de paspalum L. (Gramineae) no Sul do Brasil. Ciência Rural, Santa Maria, v. 35, n. 1, p. 56-61, 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | MACHADO, A. C. de C.; POZZOBON, M. T.; VALLS, J. F. M.; SANTOS, S. dos; RODRIGUES, L. G. Estudo da variabilidade em Paspalum (Gramineae) pela análise citogenética revela novos acessos diplóides. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 5., 2000, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2000. p. 50.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | RIBEIRO, M. R.; LIMA, G. S.; POZZOBON, T. M.; SANTOS, S. dos; PENALOZA, A. D. P. de S. Germoplasma de trigo e suas características cromossômicas após 30 anos de armazenamento. In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 175.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | NEVES, J. D. S. das; NEGRISOLI JUNIOR, A. S.; TEODORO, A. V.; GUZZO, E. C.; SANTOS, S. dos. Utilização de selantes no controle da broca-do-estipe-do-coqueiro Rhinostomus barbirostris (Fabricius) (Coleoptera: Curculionidae). In: SEMINÁRIO SOBRE INTENSIFICAÇÃO ECOLÓGICA DA FRUTICULTURA, 3; REUNIÃO COMITÊ GESTOR DO PROJETO SISTEMA ECOLOGICAMENTE INTENSIVO DE PRODUÇÃO DE FRUTAS. 3., 2004, Brasília, DF; Aracaju, SE. Seifrut: [anais..]. Aracaju, SE: Embrapa Tabuleiros Costeiros, 2014. 197 p. Editores Técnicos: Carlos Roberto Martins, Embrapa Clima Temperado; Francisco Alisson Xavier, Embrapa Mandioca; Antônio Souza do Nascimento, Embrapa Mandioca e Fruticultura.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 16. | | POZZOBON, M. T.; BIANCHETTI, L. de B.; SANTOS, S. dos; CARVALHO, S. I. C. de; REIFSCHNEIDER, F. J. B.; RIBEIRO, C. S. da C. Comportamento meiótico em acessos de Capsicum chinense Jacq. do Banco de Germoplasma da Embrapa, Brasil. Revista Brasileira de Biociencias, Porto Alegre, v. 13, n. 2, p. 96-100, abr./jun. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | OLIVEIRA, J.; RIFFEL, A.; SANTOS, S. dos; BENISIO FILHO; SANTOS, T.; RIBEIRO, T.; OLIVEIRA, D.; SILVA, W. da; COSTA, J. da; BIRKETT, M.; PICKETT, J.; SANTANA, A. Cotesia flavipes is attracted to a sesquiterpene emitted by sugarcane in response to sugarcane borer infestation. In: INTERNATIONAL CHEMICAL ECOLOGY CONFERENCE, 2013, Victoria. Abstracts... Victoria, AT: International society of Chemical Ecology, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 18. | | POZZOBON, M. T.; SANTOS, S. dos; ALVES, R. B. N.; VIEIRA, R. F.; SILVA, D. B. da; PEÑALOZA, A. P. S.; RITTER, M. R. Citogenética como ferramenta na caracterização da coleção de germoplasma de guaco (Mikania SPP.) da Embrapa. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA E O CARIBE, 10., 2015, Bento Gonçalves. Anais... Bento Gonçalves: Aptor Software, 2015. p. 285.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | POZZOBON, M. T.; SANTOS, S. dos; ALVES, R. de B. das N.; VIEIRA, R. F.; SILVA, D. B. da; PENALOZA, A. de S. Número cromossômico para acessos de germoplasma de guaco, Mikania willd. (Asteraceae). In. CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2014. Resumo. 142. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | RIBEIRO, M. R.; POZZOBON, M. T.; SANTOS, S. dos; GOEDERT, C. de O.; BRAMMER, S.; PEÑALOZA, A. del P. de S. Análise do índice meiótico em acessos de germoplasma de trigo armazenados na Colbase da Embrapa Recursos Genéticos e Biotecnologia. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 101. Também publicado no: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 120.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 32 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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