| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
06/01/2016 |
|
Data da última atualização: |
20/06/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FREIRE, M. S.; CANTUÁRIA, A. P. C.; LIMA, S. M. F.; ALMEIDA, J. A.; MURAD, A. M.; FRANCO, O. L.; REZENDE, T. M. B. |
|
Afiliação: |
MIRNA S. FREIRE, UCB; ANA PAULA C. CANTUÁRIA, UnB; STELLA M. F. LIMA, UCB; JEESER A. ALMEIDA, UCB; ANDRE MELRO MURAD, CENARGEN; OCTAVIO L. FRANCO, UCB; TAIA M. B. REZENDE, UCB. |
|
Título: |
NanoUPLC-MS(E) proteomic analysis of osteoclastogenesis downregulation by IL-4. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Journal of Proteomics, v. 131, p. 8-16, 2015. |
|
Idioma: |
Português |
|
Conteúdo: |
Bone resorption is an important factor in bone homeostasis and imbalance can cause several diseases. In osteoimmunology, IL-4 has been described as an important factor in promoting M2 macrophage profile. In order to shed some light on the effect of IL-4 on osteoclast precursors in the presence of RANKL, cytokines and nitric oxide (NO) production and the proteomic profile were analyzed. The presence of IL-4 in in vitro osteoclastogenesis provides production of TNF-?, IL-1?, IL-1?, IL-10 and IL-12 at basal cell levels. Regarding NO production, IL-4 was sufficient to increase the basal NO levels. Proteomic analyses identified 877 global proteins. IL-4 in in vitro RANKL-mediated osteoclastogenesis leads to the expression of 118 proteins. The presence of rIL-4 in in vitro rRANKL-mediated-osteoclastogenesis downregulated this process. However, the proteomics findings in the osteoclastogenesis demonstrated a much greater effect on osteoclast precursor cells than on RANKL-differentiated osteoclasts. These results suggest that the main effect of IL-4 in pre-osteoclast cells leads to a M2 macrophage activation, and this probably contributed to a reduction in osteoclastogenesis when both stimuli were used. This study noticed that IL-4 plays an important regulatory role in bone homeostasis due to its suppressive potential of precursor osteoclast cells. |
|
Palavras-Chave: |
NanoUPLC-MSE; Osteoimmunology. |
|
Thesaurus Nal: |
bone resorption; interleukin-4. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02048naa a2200241 a 4500
001 2033154
005 2024-06-20
008 2015 bl uuuu u00u1 u #d
100 1 $aFREIRE, M. S.
245 $aNanoUPLC-MS(E) proteomic analysis of osteoclastogenesis downregulation by IL-4.$h[electronic resource]
260 $c2015
520 $aBone resorption is an important factor in bone homeostasis and imbalance can cause several diseases. In osteoimmunology, IL-4 has been described as an important factor in promoting M2 macrophage profile. In order to shed some light on the effect of IL-4 on osteoclast precursors in the presence of RANKL, cytokines and nitric oxide (NO) production and the proteomic profile were analyzed. The presence of IL-4 in in vitro osteoclastogenesis provides production of TNF-?, IL-1?, IL-1?, IL-10 and IL-12 at basal cell levels. Regarding NO production, IL-4 was sufficient to increase the basal NO levels. Proteomic analyses identified 877 global proteins. IL-4 in in vitro RANKL-mediated osteoclastogenesis leads to the expression of 118 proteins. The presence of rIL-4 in in vitro rRANKL-mediated-osteoclastogenesis downregulated this process. However, the proteomics findings in the osteoclastogenesis demonstrated a much greater effect on osteoclast precursor cells than on RANKL-differentiated osteoclasts. These results suggest that the main effect of IL-4 in pre-osteoclast cells leads to a M2 macrophage activation, and this probably contributed to a reduction in osteoclastogenesis when both stimuli were used. This study noticed that IL-4 plays an important regulatory role in bone homeostasis due to its suppressive potential of precursor osteoclast cells.
650 $abone resorption
650 $ainterleukin-4
653 $aNanoUPLC-MSE
653 $aOsteoimmunology
700 1 $aCANTUÁRIA, A. P. C.
700 1 $aLIMA, S. M. F.
700 1 $aALMEIDA, J. A.
700 1 $aMURAD, A. M.
700 1 $aFRANCO, O. L.
700 1 $aREZENDE, T. M. B.
773 $tJournal of Proteomics$gv. 131, p. 8-16, 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | FERNANDES, G. R.; BARBOSA, A. E. A. D.; ALMEIDA, R. N.; CASTRO, F. F. dos S.; PONTE, M. de C. P. da; FARIA-JUNIOR, C.; MÜLLER, F. M. P.; VIANA, A. A. B.; GRATTAPAGLIA, D.; FRANCO, O. L.; ALENCAR, S. A.; DIAS, S. C. Genomic comparison among lethal invasive strains of Streptococcus pyogenes Serotype M1. Frontiers in Microbiology, v. 8, 1993, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
