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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
06/01/2016 |
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Data da última atualização: |
20/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FREIRE, M. S.; CANTUÁRIA, A. P. C.; LIMA, S. M. F.; ALMEIDA, J. A.; MURAD, A. M.; FRANCO, O. L.; REZENDE, T. M. B. |
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Afiliação: |
MIRNA S. FREIRE, UCB; ANA PAULA C. CANTUÁRIA, UnB; STELLA M. F. LIMA, UCB; JEESER A. ALMEIDA, UCB; ANDRE MELRO MURAD, CENARGEN; OCTAVIO L. FRANCO, UCB; TAIA M. B. REZENDE, UCB. |
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Título: |
NanoUPLC-MS(E) proteomic analysis of osteoclastogenesis downregulation by IL-4. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 131, p. 8-16, 2015. |
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Idioma: |
Português |
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Conteúdo: |
Bone resorption is an important factor in bone homeostasis and imbalance can cause several diseases. In osteoimmunology, IL-4 has been described as an important factor in promoting M2 macrophage profile. In order to shed some light on the effect of IL-4 on osteoclast precursors in the presence of RANKL, cytokines and nitric oxide (NO) production and the proteomic profile were analyzed. The presence of IL-4 in in vitro osteoclastogenesis provides production of TNF-?, IL-1?, IL-1?, IL-10 and IL-12 at basal cell levels. Regarding NO production, IL-4 was sufficient to increase the basal NO levels. Proteomic analyses identified 877 global proteins. IL-4 in in vitro RANKL-mediated osteoclastogenesis leads to the expression of 118 proteins. The presence of rIL-4 in in vitro rRANKL-mediated-osteoclastogenesis downregulated this process. However, the proteomics findings in the osteoclastogenesis demonstrated a much greater effect on osteoclast precursor cells than on RANKL-differentiated osteoclasts. These results suggest that the main effect of IL-4 in pre-osteoclast cells leads to a M2 macrophage activation, and this probably contributed to a reduction in osteoclastogenesis when both stimuli were used. This study noticed that IL-4 plays an important regulatory role in bone homeostasis due to its suppressive potential of precursor osteoclast cells. |
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Palavras-Chave: |
NanoUPLC-MSE; Osteoimmunology. |
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Thesaurus Nal: |
bone resorption; interleukin-4. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02048naa a2200241 a 4500
001 2033154
005 2023-03-20
008 2015 bl uuuu u00u1 u #d
100 1 $aFREIRE, M. S.
245 $aNanoUPLC-MS(E) proteomic analysis of osteoclastogenesis downregulation by IL-4.$h[electronic resource]
260 $c2015
520 $aBone resorption is an important factor in bone homeostasis and imbalance can cause several diseases. In osteoimmunology, IL-4 has been described as an important factor in promoting M2 macrophage profile. In order to shed some light on the effect of IL-4 on osteoclast precursors in the presence of RANKL, cytokines and nitric oxide (NO) production and the proteomic profile were analyzed. The presence of IL-4 in in vitro osteoclastogenesis provides production of TNF-?, IL-1?, IL-1?, IL-10 and IL-12 at basal cell levels. Regarding NO production, IL-4 was sufficient to increase the basal NO levels. Proteomic analyses identified 877 global proteins. IL-4 in in vitro RANKL-mediated osteoclastogenesis leads to the expression of 118 proteins. The presence of rIL-4 in in vitro rRANKL-mediated-osteoclastogenesis downregulated this process. However, the proteomics findings in the osteoclastogenesis demonstrated a much greater effect on osteoclast precursor cells than on RANKL-differentiated osteoclasts. These results suggest that the main effect of IL-4 in pre-osteoclast cells leads to a M2 macrophage activation, and this probably contributed to a reduction in osteoclastogenesis when both stimuli were used. This study noticed that IL-4 plays an important regulatory role in bone homeostasis due to its suppressive potential of precursor osteoclast cells.
650 $abone resorption
650 $ainterleukin-4
653 $aNanoUPLC-MSE
653 $aOsteoimmunology
700 1 $aCANTUÁRIA, A. P. C.
700 1 $aLIMA, S. M. F.
700 1 $aALMEIDA, J. A.
700 1 $aMURAD, A. M.
700 1 $aFRANCO, O. L.
700 1 $aREZENDE, T. M. B.
773 $tJournal of Proteomics$gv. 131, p. 8-16, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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