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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
25/11/2015 |
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Data da última atualização: |
04/03/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
LOUZAN, A. L. R. M.; CAITANO, M. A. B.; SANTOS, M. G. dos; VERBISCK, N. V.; ROSINHA, G. M. S. |
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Afiliação: |
UFMS; UFMS; MARIA GORETTI DOS SANTOS, CNPGC; NEWTON VALERIO VERBISCK, CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
Maldi-tof mass spectrometry identification and differentiation of Brucella species. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: MEDITERRANEAN SEA REGION COUNTRIES MASS SPECTROMETRY WORKSHOP (MEDMS III), 3., 2015., Athens, Greece. [Poster]. Athens, Greece: Univeristy of Athens, 2015. p. 61. |
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Idioma: |
Inglês |
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Conteúdo: |
Brucella species are Gram-negative bacteria that cause BrucelIosis, also known as Mediterranean or Malta fever, a highly contagious and chronic disease that occurs worldwide. Brucellosis may affect human after close contact with infected animal secretions and by ingestion of contaminated animal products, such as those made with unpausterized milk or undercooked meat. Livestock, including cattle, sheep, goats, pigs and dogs are the most common reservoirs for Brucella bacteria, which the detection stilI relies on microbiological culture and serological tests. However, in the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) has been used for microrganism identification at genus and species leveI. CurrentIy, MALDI-TOF MS is being used as a rapid, cost effective and reliable methodology for bacteria identification even at the subspecies leveI. Here we show that MALDI- TOF MS is useful for Brucella identification and differentiation, B. abortus (strains 544, S2308, 19 and RB51), B. suis, B. ovis and B. canis reference samples were grown and, after ethanol inactivation, proteins were extracted with formic acidlacetonitrile. Mass spectra were acquired with alpha-cyano-4-hydroxy cinnamic acid in an Autoflex III Smartbeam mass spectrometer (Bruker Daltonics). MALDI Biotyper software (Bruker Daltonics) dendogram analysis provided that the mass spectra profiles (MSPs) obtained can distinguish alI the species of Brucella studied herein. Moreover, fingerprints of the wild-type S2308 and vaccine 19 and RB51 B. abortus strains are clearly different. The next step wilI be to use these MSPs to detect and identify Brucella from infected cattle. Financial support: Embrapa, FUNDECT, CNPq and CAPES. MenosBrucella species are Gram-negative bacteria that cause BrucelIosis, also known as Mediterranean or Malta fever, a highly contagious and chronic disease that occurs worldwide. Brucellosis may affect human after close contact with infected animal secretions and by ingestion of contaminated animal products, such as those made with unpausterized milk or undercooked meat. Livestock, including cattle, sheep, goats, pigs and dogs are the most common reservoirs for Brucella bacteria, which the detection stilI relies on microbiological culture and serological tests. However, in the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) has been used for microrganism identification at genus and species leveI. CurrentIy, MALDI-TOF MS is being used as a rapid, cost effective and reliable methodology for bacteria identification even at the subspecies leveI. Here we show that MALDI- TOF MS is useful for Brucella identification and differentiation, B. abortus (strains 544, S2308, 19 and RB51), B. suis, B. ovis and B. canis reference samples were grown and, after ethanol inactivation, proteins were extracted with formic acidlacetonitrile. Mass spectra were acquired with alpha-cyano-4-hydroxy cinnamic acid in an Autoflex III Smartbeam mass spectrometer (Bruker Daltonics). MALDI Biotyper software (Bruker Daltonics) dendogram analysis provided that the mass spectra profiles (MSPs) obtained can distinguish alI the species of Brucella studied he... Mostrar Tudo |
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Thesagro: |
Brucella. |
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Thesaurus Nal: |
Brucellosis; Spectroscopy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02466nam a2200193 a 4500
001 2029543
005 2016-03-04
008 2015 bl uuuu u00u1 u #d
100 1 $aLOUZAN, A. L. R. M.
245 $aMaldi-tof mass spectrometry identification and differentiation of Brucella species.$h[electronic resource]
260 $aIn: MEDITERRANEAN SEA REGION COUNTRIES MASS SPECTROMETRY WORKSHOP (MEDMS III), 3., 2015., Athens, Greece. [Poster]. Athens, Greece: Univeristy of Athens, 2015. p. 61.$c2015
520 $aBrucella species are Gram-negative bacteria that cause BrucelIosis, also known as Mediterranean or Malta fever, a highly contagious and chronic disease that occurs worldwide. Brucellosis may affect human after close contact with infected animal secretions and by ingestion of contaminated animal products, such as those made with unpausterized milk or undercooked meat. Livestock, including cattle, sheep, goats, pigs and dogs are the most common reservoirs for Brucella bacteria, which the detection stilI relies on microbiological culture and serological tests. However, in the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) has been used for microrganism identification at genus and species leveI. CurrentIy, MALDI-TOF MS is being used as a rapid, cost effective and reliable methodology for bacteria identification even at the subspecies leveI. Here we show that MALDI- TOF MS is useful for Brucella identification and differentiation, B. abortus (strains 544, S2308, 19 and RB51), B. suis, B. ovis and B. canis reference samples were grown and, after ethanol inactivation, proteins were extracted with formic acidlacetonitrile. Mass spectra were acquired with alpha-cyano-4-hydroxy cinnamic acid in an Autoflex III Smartbeam mass spectrometer (Bruker Daltonics). MALDI Biotyper software (Bruker Daltonics) dendogram analysis provided that the mass spectra profiles (MSPs) obtained can distinguish alI the species of Brucella studied herein. Moreover, fingerprints of the wild-type S2308 and vaccine 19 and RB51 B. abortus strains are clearly different. The next step wilI be to use these MSPs to detect and identify Brucella from infected cattle. Financial support: Embrapa, FUNDECT, CNPq and CAPES.
650 $aBrucellosis
650 $aSpectroscopy
650 $aBrucella
700 1 $aCAITANO, M. A. B.
700 1 $aSANTOS, M. G. dos
700 1 $aVERBISCK, N. V.
700 1 $aROSINHA, G. M. S.
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| 4. |  | MENEZES, C. Meliponicultura. Belém, PA: Embrapa Amazônia Oriental, 2014. 1 folder. Publicação também em inglês.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | MENEZES, C.; VOLLET NETO, A. Instalação e povoamento do meliponário. In: DRUMOND, P. M.; CARVALHO-ZILSE, G. A.; WITTER, S.; ALVES, R. M. de O.; DRUMMOND, M. S. (ed.). Meliponicultura: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa, 2024. (Coleção 500 perguntas, 500 respostas). p. 105-116.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | MENEZES, C.; VOLLET NETO, A. Multiplicação de colônias. In: DRUMOND, P. M.; CARVALHO-ZILSE, G. A.; WITTER, S.; ALVES, R. M. de O.; DRUMMOND, M. S. (ed.). Meliponicultura: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa, 2024. (Coleção 500 perguntas, 500 respostas). p. 117-127.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 15. | | MENEZES, C. B. de (ed.). Sorgo granífero: estenda sua safrinha com segurança. Sete Lagoas: Embrapa Milho e Sorgo, 2015. 65 p. (Embrapa Milho e Sorgo. Documentos, 176). Autores: Alexandre Ferreira da Silva, Álvaro Vilela de Resende, André May, Antônio Marcos Coelho, Cicero Beserra de Menezes, Dagma Dionisia da Silva, Emerson Borghi, Evandro Chartuni Mantonvani, Flávio Dessaune Tardin, Flávia Cristina dos...| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 16. | | CONTRERA, F. A. L.; MENEZES, C. Alimentador externo para abelhas-sem-ferrão: estudo de caso com a abelha sem ferrão Melipona flavolineata (Uruçu-amarela). In: CONGRESSO BRASILEIRO DE APICULTURA, 20.; CONGRESSO BRASILEIRO DE MELIPONICULTURA, 6., 2014, Belém, PA. Sustentabilidade, tecnologia e mercados. Belém, PA: CBA, [2014]. p. 255.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 543 | |
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