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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/11/2015 |
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Data da última atualização: |
26/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WIEBKE-STROHM, B.; RECHENMACHER, C.; OLIVEIRA, L. A. de; GODOY, C. V.; ZANETTINI, M. H. B. |
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Afiliação: |
BEATRIZ WIEBKE-STROHM, UFRGS; CILIANA RECHENMACHER, UFRGS; LUISA ABRUZZI DE OLIVEIRA, UFRGS; CLAUDIA VIEIRA GODOY, CNPSO; MARIA HELENA BODANESE ZANETTINI, UFRGS. |
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Título: |
Phakopsora pachyrhizi infection bioassay in detached soybean transgenic leaves For candidate gene validation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Bio-protocol, v. 5, n. 14, e1540, Jul. 2015. |
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ISSN: |
2331-8325 |
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Idioma: |
Inglês |
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Conteúdo: |
Soybean (Glycine max) is one of the most important crops in the world. Phakopsora pachyrhizi is a plant pathogenic basidiomycete fungus that infects soybean, causing Asian Soybean Rust (ASR) disease and affecting production. Here, we describe how to prepare the plant material and the uredospore suspension (from spores harvested from leaves exhibiting sporulating uredinia) for in vitro leaf infection. Plant material is sprayed with the uredospore suspension and incubated for 12 days. During the incubation period, the presence of lesions and pustules is visually verified. After this incubation period, the leaves are classified according to the lesion type. The number of uredospores per cm2 of leaf was also estimated. The detached-leaf assay is routinely used to test fungicide efficiency (Scherb and Mehl, 2006). Detached-leaf, greenhouse and field results have been shown to be significantly correlated (Twizeyimana et al., 2007). The present protocol was adapted from the two publications cited above. The usefulness of this approach for studying P. pachyrhyzi infection on transgenic soybean was previously demonstrated by our research team (Wiebke-Strohm et al., 2012; Bencke-Malato et al., 2014). |
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Palavras-Chave: |
Ferrugem asiática da soja. |
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Thesagro: |
Doença de planta; Fungo; Phakopsora pachyrhizi. |
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Thesaurus Nal: |
Microbial growth; Plant diseases and disorders; Soybean rust. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02016naa a2200265 a 4500
001 2029004
005 2017-07-26
008 2015 bl uuuu u00u1 u #d
022 $a2331-8325
100 1 $aWIEBKE-STROHM, B.
245 $aPhakopsora pachyrhizi infection bioassay in detached soybean transgenic leaves For candidate gene validation.$h[electronic resource]
260 $c2015
520 $aSoybean (Glycine max) is one of the most important crops in the world. Phakopsora pachyrhizi is a plant pathogenic basidiomycete fungus that infects soybean, causing Asian Soybean Rust (ASR) disease and affecting production. Here, we describe how to prepare the plant material and the uredospore suspension (from spores harvested from leaves exhibiting sporulating uredinia) for in vitro leaf infection. Plant material is sprayed with the uredospore suspension and incubated for 12 days. During the incubation period, the presence of lesions and pustules is visually verified. After this incubation period, the leaves are classified according to the lesion type. The number of uredospores per cm2 of leaf was also estimated. The detached-leaf assay is routinely used to test fungicide efficiency (Scherb and Mehl, 2006). Detached-leaf, greenhouse and field results have been shown to be significantly correlated (Twizeyimana et al., 2007). The present protocol was adapted from the two publications cited above. The usefulness of this approach for studying P. pachyrhyzi infection on transgenic soybean was previously demonstrated by our research team (Wiebke-Strohm et al., 2012; Bencke-Malato et al., 2014).
650 $aMicrobial growth
650 $aPlant diseases and disorders
650 $aSoybean rust
650 $aDoença de planta
650 $aFungo
650 $aPhakopsora pachyrhizi
653 $aFerrugem asiática da soja
700 1 $aRECHENMACHER, C.
700 1 $aOLIVEIRA, L. A. de
700 1 $aGODOY, C. V.
700 1 $aZANETTINI, M. H. B.
773 $tBio-protocol$gv. 5, n. 14, e1540, Jul. 2015.
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Embrapa Soja (CNPSO) |
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| 1. |  | SAMPAIO, D. R.; FERREIRA, E. A. B.; MARTINS, E. de S.; CARDOSO, W. dos S.; PASSO, D. P.; NASCIMENTO, G. de J. Matéria orgânica e capacidade de troca catiônica em diferentes sistemas de manejo na região do Cerrado. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 165-166 (Embrapa Cerrados. Documentos, 243).| Tipo: Resumo em Anais de Congresso |
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