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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
13/11/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M. |
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Afiliação: |
FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ERGUN KAYA, National Center for Genetic Resources Preservation; LÍVIA DE JESUS VIEIRA; EVERTON HILO DE SOUZA; VANUSIA BATISTA DE OLIVEIRA AMORIM; DIANNE SKOGERBOE, National Center for Genetic Resources Preservation; TRACIE MATSUMOTO, Pacific Basin Agriculture Research Center; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; MARIA M. JENDEREK, National Center for Genetic Resources Preservation. |
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Título: |
Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015. |
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ISSN: |
0167-6857 |
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DOI: |
10.1007/s11240-015-0899-8 |
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Idioma: |
Português |
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Conteúdo: |
Germplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue. MenosGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the ... Mostrar Tudo |
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Thesagro: |
Abacaxi; Ananás Comosus. |
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Thesaurus Nal: |
Histology; Pineapples. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02476naa a2200301 a 4500
001 2028518
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a0167-6857
024 7 $a10.1007/s11240-015-0899-8$2DOI
100 1 $aSOUZA, F. V. D.
245 $aDroplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes.$h[electronic resource]
260 $c2015
520 $aGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue.
650 $aHistology
650 $aPineapples
650 $aAbacaxi
650 $aAnanás Comosus
700 1 $aKAYA, E.
700 1 $aVIEIRA, L. de J.
700 1 $aSOUZA, E. H. de
700 1 $aAMORIM, V. B. de O.
700 1 $aSKOGERBOE, D.
700 1 $aMATSUMOTO, T.
700 1 $aALVES, A. A. C.
700 1 $aLEDO, C. A. da S.
700 1 $aJENDEREK, M. M.
773 $tPlant Cell, Tissue and Organ Culture$gv.123, n.2, nov. 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 9 | |
| 3. |  | MACEDO, R. S. C.; MARQUES, J. R. F.; OHAZE, M. M. M.; BARBOSA, T. M. F. Conservação de recursos genéticos de bovídeos de interesse socioeconômico para a Amazônia Oriental/BAGAM. In: SEMINÁRIO DE INICIAÇÃO CIENTIFICA DA FCAP, 10.; SEMINÁRIO DE INICIAÇÃO CIENTIFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 4., 2000, Belém, PA. Resumos. Belém, PA: FCAP, 2000. p. 396.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | OHAZE, M. M. M.; MARQUES, J. R. F.; MACEDO, R. S. C.; BARBOSA, T. M. F. Conservação de recursos genéticos de equídeos de interesse socioeconômico para a Amazônia Oriental. In: SEMINÁRIO DE INICIAÇÃO CIENTIFICA DA FCAP, 10.; SEMINÁRIO DE INICIAÇÃO CIENTIFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 4., 2000, Belém, PA. Resumos. Belém, PA: FCAP, 2000. p. 372.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | CELESTINO FILHO, P.; QUANZ, D.; BARBOSA, T. M. F.; NAHUM, B. de S. Roça permanente de lavoura branca (RPLB) na transamazônica: alternativas para a produção de culturas alimentares em áreas alteradas. Revista Amazônia, Belém, PA, ano 4, n. 14, p. 30-31, 2009.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | BARBOSA, T. M. F.; COSTA, M. R.; MARQUES, J. R. F.; OHAZE, M. M. M.; MACEDO, R. S. C. Uso de marcadores moleculares RAPD na caracterização genética de bubalinos conservados no BAGAM. In: SEMINÁRIO DE INICIAÇÃO CIENTIFICA DA FCAP, 10.; SEMINÁRIO DE INICIAÇÃO CIENTIFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 4., 2000, Belém, PA. Resumos. Belém, PA: FCAP, 2000. p. 240.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | CELESTINO FILHO, P.; QUANZ, D.; BARBOSA, T. M. F.; SCOPEL, E. J.; VEIGA, J. B. da; SIST, P. L. J.; TOURRAND, J.-F.; NAHUM, B. de S. Plantio direto em áreas alteradas na agricultura familiar. Belém, PA: Embrapa Amazônia Oriental: FLOAGRI: CIRAD, 2009. 23 p. il. Cartilha.| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | BARBOSA, T. M. F.; TOURRAND, J.-F.; NAHUM, B. de S.; VEIGA, J. B. da; QUANZ, D.; SIST, P.; SCOPEL, E. The start of sustainability in Amazonia? A new paradigm based on fertility for sustainable land management. In: EUROPEAN IFSA SYMPOSIUM, 8., 2008, Clermont Ferrand. Empowerment of the rural actors: a renewal of farming systems perspectives. [S.l.]: IFSA, 2008. p. 591-600.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | BARBOSA, T. M. F.; TOURRAND, J. F.; NAHUM, B. S.; VEIGA, J. B. da; QUANZ, D.; SIST, P.; SCOPEL, E.; HOMEM, V. S. F. Well-being, work comfort and food security are better than maximizing production in the Amazon. In: WORLD CONGRESS OF RURAL SOCIOLOGY, 12., 2008, Goyang. Envisioning prosperous rural futures in a globalizing world. [S.l.]: IRSA, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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