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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
15/10/2015 |
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Data da última atualização: |
15/10/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS, D. F.; MATTHIENSEN, A.; COLVARA, W.; VOTTO, A. P. S. de; TRINDADE, G. S.; SILVA, P. E. A. de; YUNES, J. S. |
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Afiliação: |
DANIELA FERNANDES RAMOS, UFRGS; ALEXANDRE MATTHIENSEN, CNPSA; WILSON COLVARA, UFRGS; ANA PAULA SOUZA DE VOTTO, UFRGS; GILMA SANTOS TRINDADE, UFRGS; PEDRO EDUARDO ALMEIDA DA SILVA; JOÃO SARKIS YUNES, UFRGS. |
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Título: |
Antimycobacterial and cytotoxicity activity of microcystins. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Venomous Animals and Toxins including Tropical Diseases, v. 21, n. 9, 2015. |
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DOI: |
10.1186/s40409-015-0009-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods: The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terrae, M. chelonae and M. kansasii. The cytotoxicity assay was performed by trypan blue exclusion against the HTC cell line. Results: Antimicrobial activity was observed in the hexanic extract of M. aeruginosa (RST 9501 strain) against M. tuberculosis, including sensitive and resistant strains with minimal inhibitory concentrations (MIC) between 1.93 ?M and 0.06 ?M. The high activity of M. aeruginosa hexanic extract could be attributed to the major presence of the toxins MC-LR and [D-Leu1] MC-LR that showed activity at MIC between 53 and 0.42 ?M against tested mycobacterial strains. Even at the highest concentration tested, no toxicity of M. aeruginosa extracts was identified against HTC cells. Conclusions: These preliminary results suggest that [D-Leu1] MC-LR is a promising candidate for the development of a new antimycobacterial agent. |
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Palavras-Chave: |
Agente micobacteriano. |
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Thesagro: |
Meio Ambiente; Micobactéria. |
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Thesaurus Nal: |
Antibiotics; Cytotoxicity; Environment; Microcystis; Mycobacteriaceae; Toxicity. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02105naa a2200313 a 4500
001 2026509
005 2015-10-15
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1186/s40409-015-0009-8$2DOI
100 1 $aRAMOS, D. F.
245 $aAntimycobacterial and cytotoxicity activity of microcystins.$h[electronic resource]
260 $c2015
520 $aBackground: The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods: The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terrae, M. chelonae and M. kansasii. The cytotoxicity assay was performed by trypan blue exclusion against the HTC cell line. Results: Antimicrobial activity was observed in the hexanic extract of M. aeruginosa (RST 9501 strain) against M. tuberculosis, including sensitive and resistant strains with minimal inhibitory concentrations (MIC) between 1.93 ?M and 0.06 ?M. The high activity of M. aeruginosa hexanic extract could be attributed to the major presence of the toxins MC-LR and [D-Leu1] MC-LR that showed activity at MIC between 53 and 0.42 ?M against tested mycobacterial strains. Even at the highest concentration tested, no toxicity of M. aeruginosa extracts was identified against HTC cells. Conclusions: These preliminary results suggest that [D-Leu1] MC-LR is a promising candidate for the development of a new antimycobacterial agent.
650 $aAntibiotics
650 $aCytotoxicity
650 $aEnvironment
650 $aMicrocystis
650 $aMycobacteriaceae
650 $aToxicity
650 $aMeio Ambiente
650 $aMicobactéria
653 $aAgente micobacteriano
700 1 $aMATTHIENSEN, A.
700 1 $aCOLVARA, W.
700 1 $aVOTTO, A. P. S. de
700 1 $aTRINDADE, G. S.
700 1 $aSILVA, P. E. A. de
700 1 $aYUNES, J. S.
773 $tJournal of Venomous Animals and Toxins including Tropical Diseases$gv. 21, n. 9, 2015.
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Embrapa Suínos e Aves (CNPSA) |
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| 3. |  | FIRMINO, P. de T.; TEOTÔNIO, M. A.; SILVA, A. C.; LIMA, L. M. R.; LIMA, W. I. M. DE. Análise de qualidade de óleo de gergelim extraído em escala artesanal. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 9. 2011, Campinas. Ciência de alimentos e qualidade de vida: saúde, meio ambiente e sustentabilidade: resumos. Campinas: UNICAMP, FEA: SLACA, 2011. 1 p. 1 CD-ROM| Tipo: Resumo em Anais de Congresso |
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