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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
01/10/2015 |
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Data da última atualização: |
20/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUERRERO, E. B.; ARNEODO, J.; CAMPANHA, R. B.; OLIVEIRA, P. A. de; LABARTE, M. T. V.; CATALDI, T. R.; CAMPOS, E.; CATALDI, A.; LABARTE, C. A.; RODRIGUES, C. M.; TALIA, P. |
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Afiliação: |
Emiliano Ben Guerrero, INTA; Joel Arneodo, CONICET; RAQUEL BOMBARDA CAMPANHA, CNPAE; PATRICIA ABRAO DE OLIVEIRA, CNPAE; Mônica T. Veneziano Labate, USP; Thaís Regiani Cataldi, USP; Eleonora Campos, INTA; Angel Cataldi, INTA; Carlos A. Labate, USP; CLENILSON MARTINS RODRIGUES, CNPAE; Paola Talia, INTA. |
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Título: |
Prospection and evaluation of (Hemi) cellulolytic enzymes using untreated and pretreated biomasses in two argentinean native termites. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
PLoS ONE, v. 10, n. 8, p1-23. 23p, 2015 |
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DOI: |
10.1371/journal.pone.0136573 |
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Idioma: |
Inglês |
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Conteúdo: |
Saccharum officinarum bagasse (common name: sugarcane bagasse) and Pennisetum purpureum (also known as Napier grass) are among the most promising feedstocks for bioethanol production in Argentina and Brazil. In this study, both biomasses were assessed before and after acid pretreatment and following hydrolysis with Nasutitermes aquilinus and Cortaritermes fulviceps termite gut digestome. The chemical composition analysis of the biomasses after diluted acid pretreatment showed that the hemicellulose fraction was partially removed. The (hemi) cellulolytic activities were evaluated in bacterial culture supernatants of termite gut homogenates grown in treated and untreated biomasses. In all cases, we detected significantly higher endoglucanase and xylanase activities using pretreated biomasses compared to untreated biomasses, carboxymethylcellulose and xylan. Several protein bands with (hemi) cellulolytic activity were detected in zymograms and two-dimensional gel electrophoresis. Some proteins of these bands or spots were identified as xylanolytic peptides by mass spectrometry. Finally, the diversity of cultured cellulolytic bacterial endosymbionts associated to both Argentinean native termite species was analyzed. This study describes, for the first time, bacterial endosymbionts and endogenous (hemi) cellulases of two Argentinean native termites as well as their potential application in degradation of lignocellulosic biomass for bioethanol production. |
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Palavras-Chave: |
Bioetanol; Subproduto da cana de açúcar. |
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Thesagro: |
Pennisetum Purpureum; Saccharum Officinarum. |
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Thesaurus Nal: |
bioethanol. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02425naa a2200313 a 4500
001 2025620
005 2017-09-20
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0136573$2DOI
100 1 $aGUERRERO, E. B.
245 $aProspection and evaluation of (Hemi) cellulolytic enzymes using untreated and pretreated biomasses in two argentinean native termites.$h[electronic resource]
260 $c2015
520 $aSaccharum officinarum bagasse (common name: sugarcane bagasse) and Pennisetum purpureum (also known as Napier grass) are among the most promising feedstocks for bioethanol production in Argentina and Brazil. In this study, both biomasses were assessed before and after acid pretreatment and following hydrolysis with Nasutitermes aquilinus and Cortaritermes fulviceps termite gut digestome. The chemical composition analysis of the biomasses after diluted acid pretreatment showed that the hemicellulose fraction was partially removed. The (hemi) cellulolytic activities were evaluated in bacterial culture supernatants of termite gut homogenates grown in treated and untreated biomasses. In all cases, we detected significantly higher endoglucanase and xylanase activities using pretreated biomasses compared to untreated biomasses, carboxymethylcellulose and xylan. Several protein bands with (hemi) cellulolytic activity were detected in zymograms and two-dimensional gel electrophoresis. Some proteins of these bands or spots were identified as xylanolytic peptides by mass spectrometry. Finally, the diversity of cultured cellulolytic bacterial endosymbionts associated to both Argentinean native termite species was analyzed. This study describes, for the first time, bacterial endosymbionts and endogenous (hemi) cellulases of two Argentinean native termites as well as their potential application in degradation of lignocellulosic biomass for bioethanol production.
650 $abioethanol
650 $aPennisetum Purpureum
650 $aSaccharum Officinarum
653 $aBioetanol
653 $aSubproduto da cana de açúcar
700 1 $aARNEODO, J.
700 1 $aCAMPANHA, R. B.
700 1 $aOLIVEIRA, P. A. de
700 1 $aLABARTE, M. T. V.
700 1 $aCATALDI, T. R.
700 1 $aCAMPOS, E.
700 1 $aCATALDI, A.
700 1 $aLABARTE, C. A.
700 1 $aRODRIGUES, C. M.
700 1 $aTALIA, P.
773 $tPLoS ONE$gv. 10, n. 8, p1-23. 23p, 2015
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Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 10 | |
| 6. |  | PAES, J. M. V.; ANDRADE, A. T.; TEIXEIRA, C. M.; GAZZIERO, D. L. P.; BRIGHENTI, A. M.; ZITO, R. K. Cultivo da soja no Sistema Plantio Direto no Cerrado. Informe Agropecuário, v. 39, n. 302, p. 68-76, 2018. Título em inglês: Soybean no-tillage cropping system in the Cerrado.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Soja. |
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| 8. | | OLIVEIRA, L. H. de; GRIS, C. F.; FRONZA, V.; TEIXEIRA, C. M.; COUGO, W. G.; BALDONI, A. Densidades e cultivares de soja RR em dois anos de plantio para o Sul de Minas Gerais. In: JORNADA CIENTÍFICA E TECNOLÓGICA, 6., SIMPÓSIO DE PÓS-GRADUAÇÃO DE IFSULDEMINAS, 3., 2014, Pouso Alegre. 6 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | FONTANETTI, A.; CARVALHO, G. J. de; MORAES, S. R. G. de; ALMEIDA, K. de; TEIXEIRA, C. M. Plantas de cobertura no controle de plantas daninhas nas culturas de alface americana e repolho. Horticultura Brasileira, Brasília, v. 21, n. 2, jul. 2003. Suplemento 2. Trabalho apresentado no 43º Congresso Brasileiro de Olericultura, 2003. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 21, n. 2, p. 268, jul. 2003. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | SILVA, K. B.; BRUZI, A. T.; ZUFFO, A. M.; ZAMBIAZZI, E. V.; SOARES, I. O.; REZENDE, P. M. de; FRONZA, V.; VILELA, G. D. L.; BOTELHO, F. B. S.; TEIXEIRA, C. M.; COELHO, M. A. de O. Adaptability and phenotypic stability of soybean cultivars for grain yield and oil content. Genetics and Molecular Research, v. 15, n. 2, 11 p., Apr. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 10 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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