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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
29/09/2015 |
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Data da última atualização: |
20/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FAI, A. E. C.; SIMIQUELI, A. P. R.; GHISELLI, G.; PASTORE, G. M. |
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Afiliação: |
Ana Elizabeth Cavalcante Fai, UNICAMP; Ana Paula Resende Simiqueli, UNICAMP; GISLAINE GHISELLI, CNPAE; Gláucia Maria Pastore, UNICAMP. |
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Título: |
Sequential optimization approach for prebiotic galactooligosaccharides synthesis by Pseudozyma tsukubaensis and Pichia kluyveri. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Food Science and Technology, v. 63, p. 1214-1219, 2015. |
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DOI: |
10.1016/j.lwt.2015.04.064 |
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Idioma: |
Inglês |
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Conteúdo: |
Synthesis of the prebiotic galactooligosaccharides from lactose fermentation by Pseudozyma tsukubaensis and Pichia kluyveri was investigated. In this study, a sequential strategy of two experimental designs was used to optimize galactooligosaccharides production. First, Plackett-Burman screening design was applied to evaluate the effects of lactose, yeast extract, urea concentration, inoculum concentration, pH and agitation rate on the response of galactooligosaccharides yield synthesized by both yeasts. The preliminary Plackett-Burman design allowed the identification of the pH and urea concentration as statistically significant variables in the range studied. In order to find the optimal conditions for galactooligosaccharides synthesis, a central composite rotational design was employed. The independent variables were lactose, yeast extract and urea concentrations, and the response taken into account was galactooligosaccharides yield produced by P. tsukubaensis. The optimized condition for this prebiotic synthesis was: lactose, yeast extract and urea at concentrations of 26.0, 0.8 and 1.8 g/100 mL, respectively. Under the optimized and validated conditions, the transgalactosylation activity of P. tsukubaensis resulted in GOS yield of 28.35 GOS (g)/100 g of lactose at 24 h, with a maximum GOS production of 73.71 g/L, and approximately 50 g/100 g of lactose hydrolysis. |
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Palavras-Chave: |
Metodologia de superfície de resposta; Prebiotic; Transgalactosilação; Transgalactosylation. |
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Thesagro: |
Lactose. |
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Thesaurus Nal: |
response surface methodology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02189naa a2200241 a 4500
001 2025325
005 2017-09-20
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.lwt.2015.04.064$2DOI
100 1 $aFAI, A. E. C.
245 $aSequential optimization approach for prebiotic galactooligosaccharides synthesis by Pseudozyma tsukubaensis and Pichia kluyveri.$h[electronic resource]
260 $c2015
520 $aSynthesis of the prebiotic galactooligosaccharides from lactose fermentation by Pseudozyma tsukubaensis and Pichia kluyveri was investigated. In this study, a sequential strategy of two experimental designs was used to optimize galactooligosaccharides production. First, Plackett-Burman screening design was applied to evaluate the effects of lactose, yeast extract, urea concentration, inoculum concentration, pH and agitation rate on the response of galactooligosaccharides yield synthesized by both yeasts. The preliminary Plackett-Burman design allowed the identification of the pH and urea concentration as statistically significant variables in the range studied. In order to find the optimal conditions for galactooligosaccharides synthesis, a central composite rotational design was employed. The independent variables were lactose, yeast extract and urea concentrations, and the response taken into account was galactooligosaccharides yield produced by P. tsukubaensis. The optimized condition for this prebiotic synthesis was: lactose, yeast extract and urea at concentrations of 26.0, 0.8 and 1.8 g/100 mL, respectively. Under the optimized and validated conditions, the transgalactosylation activity of P. tsukubaensis resulted in GOS yield of 28.35 GOS (g)/100 g of lactose at 24 h, with a maximum GOS production of 73.71 g/L, and approximately 50 g/100 g of lactose hydrolysis.
650 $aresponse surface methodology
650 $aLactose
653 $aMetodologia de superfície de resposta
653 $aPrebiotic
653 $aTransgalactosilação
653 $aTransgalactosylation
700 1 $aSIMIQUELI, A. P. R.
700 1 $aGHISELLI, G.
700 1 $aPASTORE, G. M.
773 $tFood Science and Technology$gv. 63, p. 1214-1219, 2015.
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| 1. |  | FERRAZ, R. E. de O.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; MELO, V. S. P. de; DANTAS, T. V. M.; CAMINHA, C. M. M.; BARROSO, I. C. Lavagem do sêmen de reprodutores caprinos com solução Krebs-Ringer-Fosfato no controle da Artrite Encefalite Caprina (CAE). In: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 4.; FEIRA NACIONAL DO AGRONEGÓCIO DA CAPRINO-OVINOCULTURA DE CORTE, 3., 2009, João Pessoa. Anais... João Pessoa: EMEPA-PB, 2009. 3 f. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
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