| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
01/09/2015 |
|
Data da última atualização: |
29/03/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
OLIVEIRA, M. B.; LOBO JUNIOR, M.; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. |
|
Afiliação: |
MARÍLIA BARROS OLIVEIRA, UFG; MURILLO LOBO JUNIOR, CNPAF; MARIA FATIMA GROSSI DE SA, CENARGEN; SILVANA PETROFEZA, UFG. |
|
Título: |
Exogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Journal of Plant Physiology, v. 182, p. 13-22, June 2015. |
|
DOI: |
10.1016/j.jplph.2015.04.006 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem. MenosSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalan... Mostrar Tudo |
|
Palavras-Chave: |
Genes relacionadas com patogénese. |
|
Thesagro: |
Doença de planta; Feijão; Fungo; Mofo Branco; Phaseolus vulgaris; Sclerotinia sclerotiorum. |
|
Thesaurus Nal: |
Dry beans; Methyl jasmonate. |
|
Categoria do assunto: |
--
H Saúde e Patologia |
|
Marc: |
LEADER 02883naa a2200277 a 4500
001 2025021
005 2016-03-29
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jplph.2015.04.006$2DOI
100 1 $aOLIVEIRA, M. B.
245 $aExogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants.$h[electronic resource]
260 $c2015
520 $aSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem.
650 $aDry beans
650 $aMethyl jasmonate
650 $aDoença de planta
650 $aFeijão
650 $aFungo
650 $aMofo Branco
650 $aPhaseolus vulgaris
650 $aSclerotinia sclerotiorum
653 $aGenes relacionadas com patogénese
700 1 $aLOBO JUNIOR, M.
700 1 $aGROSSI-DE-SÁ, M. F.
700 1 $aPETROFEZA, S.
773 $tJournal of Plant Physiology$gv. 182, p. 13-22, June 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 5 | |
| 1. |  | COSTA, M. de A. A.; VIEIRA, C. V.; SANTOS, V. A. H. F. dos; FARIAS NETO, A. L. de; MENEGUCI, J. L. P.; ZOLIN, C. A. Comportamento ecofisiológico de Eucalyptus Urograndis submetido a diferentes níveis de estresse hídrico In: JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 2.,2013, Sinop. Anais... Sinop, MT: Embrapa Agrossilvipastoril, 2013. 1 CD.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
|   |
| 2. | | FERRER, J. L. R.; CASTRO, E. M. de C.; ALVES, J. D.; ALENCAR, M. A.; SILVA, S. da; VIEIRA, C. V.; MAGALHAES, P. C. Efeito do cálcio sobre as características anatômicas de raízes de milho (Zea mays L.) "Saracura" BRS-4154 submetido ao alagamento em casa de vegetação. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 3, n. 2, p. 172-181, 2004.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|  |
| 3. | | VIEIRA, C. V.; FERRER, J. R.; CASTRO, E. M.; ALVES, J. D.; MAGALHAES, P. C.; FRIES, D. D.; ALENCAR, M. A. Efeito do cálcio sobre as características anatômicas de raízes de milho (Zea mays L.) "Saracura" BRS-4154 submetido ao alongamento em casa de vegetação. Brazilian Journal of Plant Physiology, Campinas, v.15, p. 395, 2003. Suplemento. Edição dos resumos do IX Congresso Brasileiro de Fisiologia Vegetal, 2003, Atibaia, SP.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 4. | | COSTA, M. de A. A.; HARDT, V. A.; VIEIRA, C. V.; FARIAS NETO, A. L. de; MENEGUCI, J. L. P.; ZOLIN, C. A.; LULU, J.; PORFIRIO-DA-SILVA, V. Aspectos ecofisiológicos e de crescimento de Eucalyptus urograndis submetido a estresse hídrico com potencial para sistemas agrossilvipastoris. In: FARIAS NETO, A. L. de; NASCIMENTO, A. F. do; ROSSONI, A. L.; MAGALHÃES, C. A. de S.; ITUASSU, D. R.; HOOGERHEIDE, E. S. S.; IKEDA, F. S.; FERNANDES JUNIOR, F.; FARIA, G. R.; ISERNHAGEN, I.; VENDRUSCULO, L. G.; MORALES, M. M.; CARNEVALLI, R. A. (Ed.). Embrapa Agrossilvipastoril: primeiras contribuições para o desenvolvimento de uma agropecuária sustentável. Brasília, DF: Embrapa, 2019. pt. 4, cap. 8, p. 221-225.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Florestas. |
| |
| 5. | | COSTA, M. de A. A.; SANTOS, V. A. H. F. dos; VIEIRA, C. V.; FARIAS NETO, A. L. de; MENEGUCI, J. L. P.; ZOLIN, C. A.; LULU, J.; PORFIRIO-DA-SILVA, V. Aspectos ecofisiológicos e de crescimento de Eucalyptus urograndis submetido à suplementação hídrica em plantios clonais. Revista de Biologia Neotropical, v. 12, n. 1, p. 44-57, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Florestas. |
| |
| Registros recuperados : 5 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
