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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
24/04/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, C. F.; FELICIANO SILVA, A. E. D.; DODE, M. A. N.; RUMPF, R.; CUMPA, H. C. B.; SILVA, C. G.; PIVATO, I. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, CENARGEN; RODOLFO RUMPF, CENARGEN. |
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Título: |
Morphological characterization and conservation of bovine spermatogenic cells by refrigeration at 4°C and freezing using different cryoprotective molecules. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Cryobiology, v. 71, p. 47-53, 2015. |
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ISSN: |
0011-2240 |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2015.06.003 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The objectives of this study were study a practical method to characterize bovine spermatogenic cells and test the efficiency cells conservation by refrigeration at 4 °C and cryopreservation in different solutions using two cooling curves. Cellular identification was performing by analysis of shape, size and morphology, associated with nucleus positioning and nuclear-cytoplasm ratio (NCR). Cellular samples were kept at 4 °C for a period of 96 h in refrigeration solution and every 24 h plasma membrane and DNA integrity were evaluated. Cryopreservation of cells was carried out using solutions containing 10% Dimethyl sulfoxide, 5% Dimethylformamide, 7% Glycerol and 7% Ethylene glycol, using a controlled and non-controlled cooling curve. Results of cellular characterization demonstrated that spermatocytes II presented a cylindrical shape, NCR of 1:1.5 and diameter ranging from 14.5 to 17.5 μm. Round spermatids presented diameter ranging from 7.6 to 13.4 μm, acrosomal cap and NCR of 1:2. Elongation and elongated spermatids showed to marked divergence in shape. There was a daily significant loss of viability of cooled cells until third day of storage, however they presented 72.77 ± 5.16% viability after 4 days of storage at 4 °C. There was no difference among the cryoprotectant solutions and cooling curves. In conclusion we demonstrated that association of microscopes and staining was a practical method to identify bovine spermatogenic cells. Furthermore, refrigeration at 4 °C is an important strategy to preserve over 70% of viable cells after 4 days and cryopreservation, regardless of cryoprotectant solution or cooling curve used, can maintain over 50% of cells viable. MenosAbstract: The objectives of this study were study a practical method to characterize bovine spermatogenic cells and test the efficiency cells conservation by refrigeration at 4 °C and cryopreservation in different solutions using two cooling curves. Cellular identification was performing by analysis of shape, size and morphology, associated with nucleus positioning and nuclear-cytoplasm ratio (NCR). Cellular samples were kept at 4 °C for a period of 96 h in refrigeration solution and every 24 h plasma membrane and DNA integrity were evaluated. Cryopreservation of cells was carried out using solutions containing 10% Dimethyl sulfoxide, 5% Dimethylformamide, 7% Glycerol and 7% Ethylene glycol, using a controlled and non-controlled cooling curve. Results of cellular characterization demonstrated that spermatocytes II presented a cylindrical shape, NCR of 1:1.5 and diameter ranging from 14.5 to 17.5 μm. Round spermatids presented diameter ranging from 7.6 to 13.4 μm, acrosomal cap and NCR of 1:2. Elongation and elongated spermatids showed to marked divergence in shape. There was a daily significant loss of viability of cooled cells until third day of storage, however they presented 72.77 ± 5.16% viability after 4 days of storage at 4 °C. There was no difference among the cryoprotectant solutions and cooling curves. In conclusion we demonstrated that association of microscopes and staining was a practical method to identify bovine spermatogenic cells. Furthermore, refrigeration a... Mostrar Tudo |
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Thesagro: |
Congelamento; Criopreservação; Reprodução animal. |
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Categoria do assunto: |
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Marc: |
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Registros recuperados : 2 | |
| 1. |  | MATONYEI, T. K; SIRMAH, P. K.; SITIENEI, A. J.; OUMA, E. O.; LIGEYO, D. O.; CHEPROT, R. K.; MARITIM, K. K.; WERE, B. A.; KISINYO, P. O.; GUDU, S. O.; MAGALHAES, J. V.; GUIMARAES, C. T.; KOCHIAN, L. V. The expression of ZmMATE1 gene at seminal root tip does not explain aluminum toxicity tolerance in a Kenyan maize breeding line. International Journal of Scientific Research and Innovative Technology, v. 4, n. 3, p. 45-59, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 2. | | MATONYEI, T. K.; BARROS, B. de A.; GUIMARÃES, R. G. N.; OUMA, E. O.; CHEPROT, R. K.; APOLINÁRIO, L. C.; LIGEYO, D. O.; COSTA, M. B. R.; WERE, B. A.; KISINYO, P. O.; ONKWARE, A. O.; NODA, R. W.; GUDU, S. O.; MAGALHAES, J. V. de; GUIMARÃES, C. T. Aluminum tolerance mechanisms in Kenyan maize germplasm are independent from the citrate transporter ZmMATE1. Scientific Reports, v. 10, article 7320, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 2 | |
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