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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
24/04/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. G.; CUNHA, E. R.; BLUME, G. R.; MALAQUIAS, J. V.; BÁO, S. N.; MARTINS, C. F. |
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Afiliação: |
JUACI VITORIA MALAQUIAS, CPAC; UNIVERSIDADE DE BRASILIA; CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Cryobiology, v. 70, p. 90-94, 2015. |
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ISSN: |
0011-2240 |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2015.01.001 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique. MenosAbstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cry... Mostrar Tudo |
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Thesagro: |
Congelamento; Conservação; Criopreservação; Esperma; Inseminação artificial; Suino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02370naa a2200277 a 4500
001 2024328
005 2025-04-24
008 2015 bl uuuu u00u1 u #d
022 $a0011-2240
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2015.01.001$2DOI
100 1 $aSILVA, C. G.
245 $aCryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.$h[electronic resource]
260 $c2015
520 $aAbstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.
650 $aCongelamento
650 $aConservação
650 $aCriopreservação
650 $aEsperma
650 $aInseminação artificial
650 $aSuino
700 1 $aCUNHA, E. R.
700 1 $aBLUME, G. R.
700 1 $aMALAQUIAS, J. V.
700 1 $aBÁO, S. N.
700 1 $aMARTINS, C. F.
773 $tCryobiology$gv. 70, p. 90-94, 2015.
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| Registros recuperados : 4 | |
| 1. |  | SILVA, W. V. da; ZIEMNICZAK, H. M.; BACHA, F. B.; FERNANDES, R. B. S.; FUJIMOTO, R. Y.; SOUSA, R. M.; SATURINO, K. C.; HONORATO, C. A. Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations. Semina: ciências agrárias, v. 42, n. 5, p. 2993-3006, set./out. 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 2. | | PEREIRA, L. DE A.; AGUIAR, J. C. B.; RITA, L. P. S.; GUIMARÃES, R. G.; GONÇALVES, B. S.; MACHADO, D. P.; SILVA, B. L.; SILVA, L. S. C. V. DA; SILVA, W. V. DA; CURADO, F. F. Avaliação da execução orçamentária e financeira do Programa Nacional de Alimentação Escolar no Instituto Federal de Alagoas. Revista Delos, v. 18, n. 63, e3644, 2025.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Alimentos e Territórios. |
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| 3. | | AGUIAR, J. C. B.; PEREIRA, L. DE A.; SILVA, L. S. C. V. DA; SILVA, W. V. DA; CURADO, F. F.; SILVA, B. L.; SÁ JÚNIOR, A. N.; SALDANHA, P. T. M. Contribuições do estado da arte sobre as políticas públicas para a agricultura familiar após a agenda 2030 das Nações Unidas. International Journal of Scientific Management and Tourism, v. 10, n. 3, 2024. 20 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Alimentos e Territórios. |
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| 4. | | AGUIAR, J. C. B.; SILVA, L. S. C. V. DA; SILVA, W. V. DA; CURADO, F. F.; LIMA, P. H. C.; PEREIRA, L. DE A.; SILVA, B. L.; RITA, L. P. S.; PINTO, I. M. B. S. Temas centrais da relação entre agrotóxicos e câncer: uma revisão sistemática de literatura. São José dos Pinhais: Seven Editora, 2023. Cap. 48. 26 p.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Alimentos e Territórios. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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