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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
24/04/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. G.; CUNHA, E. R.; BLUME, G. R.; MALAQUIAS, J. V.; BÁO, S. N.; MARTINS, C. F. |
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Afiliação: |
JUACI VITORIA MALAQUIAS, CPAC; UNIVERSIDADE DE BRASILIA; CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Cryobiology, v. 70, p. 90-94, 2015. |
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ISSN: |
0011-2240 |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2015.01.001 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique. MenosAbstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cry... Mostrar Tudo |
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Thesagro: |
Congelamento; Conservação; Criopreservação; Esperma; Inseminação artificial; Suino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02370naa a2200277 a 4500
001 2024328
005 2025-04-24
008 2015 bl uuuu u00u1 u #d
022 $a0011-2240
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2015.01.001$2DOI
100 1 $aSILVA, C. G.
245 $aCryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.$h[electronic resource]
260 $c2015
520 $aAbstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.
650 $aCongelamento
650 $aConservação
650 $aCriopreservação
650 $aEsperma
650 $aInseminação artificial
650 $aSuino
700 1 $aCUNHA, E. R.
700 1 $aBLUME, G. R.
700 1 $aMALAQUIAS, J. V.
700 1 $aBÁO, S. N.
700 1 $aMARTINS, C. F.
773 $tCryobiology$gv. 70, p. 90-94, 2015.
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| 8. |  | FONSECA, J. F. da; CRUZ, R. C.; MANCIO, A. B.; ESTEVES, L. V.; BRANDÃO, F. Z.; FACO, O. Comportamento estral, dinâmica ovulatória e descarga de muco cervical em cabras Toggenburg submetidas à indução de estro com diferentes gonadotrofinas. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012. SBTE 067. p. 361.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | FONSECA, J. F. da; CAVALCANTI, A. S.; BARTZ, C.; SILVA, A. L. S.; NOGUEIRA, L. A. G.; BRANDÃO, F. Z. Diferentes doses de dinoprost induzem estro eficientemente no início do ciclo estral em ovelhas. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 17., 2007, Curitiba. Resumos...Belo Horizonte: CBRA, 2007. p. 145.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | VENTURI, S. S.; SOARES, I. R.; RODRIGUES, A. L. R.; CARDOSO, E. S.; FONSECA, J. F. da; BRANDÃO, F. Z. Efeito da suplementação calórica sobre a taxa de ovulação e parâmetros metabólicos em ovelhas. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 19., 2011, Recife. Anais... Belo Horizonte: CBRA, 2011. p. 270. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | PINNA, A. E.; BRANDÃO, F. Z.; CAVALCANTI, A. S.; BORGES, A. M.; LOUREIRO, A. A. A.; FONSECA, J. F. da. Fertilidade de ovelhas cíclicas submetidas à sincronização do estro utilizando implantes intravaginais (CIDR) novos ou reutilizados. Acta Scientiae Veterinariae Porto Alegre, v. 36, Supl. 2, p. s581, 2008. Edição dos Resumos XXII Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | SOUZA, J. M. G.; CORDEIRO, R. G.; FONSECA, J. F. da; BRANDÃO, F. Z.; BRUSCHI, J. H. Hormonal induction of lactation in non-pregnant Saanen and Toggenburg goats. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23., 2008, Querétaro, México. Sustainable goat production: challenges and opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. p. 124. Abstract 123.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 275 | |
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