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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
24/03/2015 |
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Data da última atualização: |
24/03/2015 |
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Autoria: |
HERINGER, A. S.; STEINMARCHER, D. A.; FRAGA, H. P. F.; VIEIRA, L. N.; MONTAGNA, T.; QUINGA, L. A. P.; QUOIRIN, M. G. G.; JIMENEZ, V. M.; GUERRA, M. P. |
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Título: |
Improved high-efficiency protocol for somatic embryogenesis in peach palm (Bactris gasipaes Kunth) using RITA® temporary immersion system. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Scientia Horticulturae, v. 179, p. 284-292, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
tBactris gasipaes Kunth (Arecaceae) is an Amazonian palm cultivated mainly for the production of fruitsand heart of palm. Biotechnological tools based on somatic embryogenesis (SE) can be used for improve-ment and germplasm conservation of this species. The efficiency of different culture systems and culturemedium composition were evaluated to optimize the efficiency of SE. The study also evaluated globalDNA methylation levels, total protein and starch contents, and alcohol dehydrogenase (ADH) activity. TheRITA®bioreactor showed enhanced multiplication of somatic embryos with increased protein, starch lev-els and ADH activity. Furthermore, a low DNA global methylation rate (27.52%) was observed, suggestingits relationship with the expression of proteins associated with the maturation of somatic embryos andtheir subsequent conversion to plantlets. Somatic embryo maturation was successfully achieved on Petridishes and abscisic acid (ABA) supplementation in the culture medium. Chlorophyll pigmented somaticembryos were obtained in Petri dishes containing PGR-free culture medium, and RITA®provided thegreatest number of plantlets to be acclimatized after reaching 6?7 cm in length. In the acclimatizationstep, the best results were obtained with plantlets derived from RITA®after reaching 6?7 cm in length.These new findings shed light on the importance of defining adequate culture systems for differentsteps of in vitro regenerative protocols and represent a breakthrough in the in vitro morphogenesis pro-cess of peach palm, resulting in a large number of somatic plantlets with enhanced physiological andmorphological features. MenostBactris gasipaes Kunth (Arecaceae) is an Amazonian palm cultivated mainly for the production of fruitsand heart of palm. Biotechnological tools based on somatic embryogenesis (SE) can be used for improve-ment and germplasm conservation of this species. The efficiency of different culture systems and culturemedium composition were evaluated to optimize the efficiency of SE. The study also evaluated globalDNA methylation levels, total protein and starch contents, and alcohol dehydrogenase (ADH) activity. TheRITA®bioreactor showed enhanced multiplication of somatic embryos with increased protein, starch lev-els and ADH activity. Furthermore, a low DNA global methylation rate (27.52%) was observed, suggestingits relationship with the expression of proteins associated with the maturation of somatic embryos andtheir subsequent conversion to plantlets. Somatic embryo maturation was successfully achieved on Petridishes and abscisic acid (ABA) supplementation in the culture medium. Chlorophyll pigmented somaticembryos were obtained in Petri dishes containing PGR-free culture medium, and RITA®provided thegreatest number of plantlets to be acclimatized after reaching 6?7 cm in length. In the acclimatizationstep, the best results were obtained with plantlets derived from RITA®after reaching 6?7 cm in length.These new findings shed light on the importance of defining adequate culture systems for differentsteps of in vitro regenerative protocols and represent a breakthrough in the in vit... Mostrar Tudo |
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Palavras-Chave: |
Embriogênese somática; Espécie florestal; Pupunheira. |
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Thesagro: |
Bactris Gasipaes; Espécie Nativa; Pupunha. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Florestas (CNPF) |
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