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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
23/01/2015 |
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Data da última atualização: |
04/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GAMA, R. N. C. S.; SANTOS, C. A. F.; DIAS, R. de C. S.; ALVES, J. C. S. F.; NOGUEIRA, T. O. |
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Afiliação: |
R. N. C. S. GAMA; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; RITA DE CASSIA SOUZA DIAS, CPATSA; J. C. S. F. ALVES; T. O. NOGUEIRA. |
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Título: |
Microsatellite markers linked to the locus of the watermelon fruit stripe pattern. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n. 1, p. 269-276, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Agronomic performance and external and internal appearance of watermelon (Citrullus lanatus) fruit are important traits that should be taken into consideration during the development of a new cultivar, as well as being the principal identification elements used by the consumer, which are based on the external appearance and quality of the fruit. Externally, the fruit can be characterized in terms of the shape, the color of the lower rind, and the presence of grooves and stripes, the stripes can be classified as clearly defined or diffuse. The objective of this study was to identify microsatellite markers linked to the stripe pattern of watermelon fruit to support watermelon improvement programs, with the selection of this characteristic in the plantlet stage. F1 and F2 populations, result of a cross between the cultivars BRS Opara (clearly defined stripes) and Pérola (diffuse stripes), were phenotyped for their fruit stripe pattern. The CTAB 2X protocol was used for DNA extraction and 116 microsatellite markers were examined in a group of F2 plants that had fruit with well-defined stripes and fruit with diffuse stripes. The microsatellite loci MCPI_05 and MCPI_16 exhibited a linkage to the stripe pattern at a distance of 1.5 and 1.8 cM, respectively, with LOD scores of 39.28 and 38.11, respectively, which were located on chromosome six of the watermelon genome. These markers can be used in marker-assisted selection in watermelon improvement programs, by various research institutions. MenosAgronomic performance and external and internal appearance of watermelon (Citrullus lanatus) fruit are important traits that should be taken into consideration during the development of a new cultivar, as well as being the principal identification elements used by the consumer, which are based on the external appearance and quality of the fruit. Externally, the fruit can be characterized in terms of the shape, the color of the lower rind, and the presence of grooves and stripes, the stripes can be classified as clearly defined or diffuse. The objective of this study was to identify microsatellite markers linked to the stripe pattern of watermelon fruit to support watermelon improvement programs, with the selection of this characteristic in the plantlet stage. F1 and F2 populations, result of a cross between the cultivars BRS Opara (clearly defined stripes) and Pérola (diffuse stripes), were phenotyped for their fruit stripe pattern. The CTAB 2X protocol was used for DNA extraction and 116 microsatellite markers were examined in a group of F2 plants that had fruit with well-defined stripes and fruit with diffuse stripes. The microsatellite loci MCPI_05 and MCPI_16 exhibited a linkage to the stripe pattern at a distance of 1.5 and 1.8 cM, respectively, with LOD scores of 39.28 and 38.11, respectively, which were located on chromosome six of the watermelon genome. These markers can be used in marker-assisted selection in watermelon improvement programs, by various research inst... Mostrar Tudo |
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Palavras-Chave: |
Fruit external pattern; Marcadores microssatélites; Melhoramento genético; Watermelon. |
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Thesagro: |
Citrullus Lanatus; Melancia. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02247naa a2200241 a 4500
001 2006536
005 2016-02-04
008 2015 bl uuuu u00u1 u #d
100 1 $aGAMA, R. N. C. S.
245 $aMicrosatellite markers linked to the locus of the watermelon fruit stripe pattern.$h[electronic resource]
260 $c2015
520 $aAgronomic performance and external and internal appearance of watermelon (Citrullus lanatus) fruit are important traits that should be taken into consideration during the development of a new cultivar, as well as being the principal identification elements used by the consumer, which are based on the external appearance and quality of the fruit. Externally, the fruit can be characterized in terms of the shape, the color of the lower rind, and the presence of grooves and stripes, the stripes can be classified as clearly defined or diffuse. The objective of this study was to identify microsatellite markers linked to the stripe pattern of watermelon fruit to support watermelon improvement programs, with the selection of this characteristic in the plantlet stage. F1 and F2 populations, result of a cross between the cultivars BRS Opara (clearly defined stripes) and Pérola (diffuse stripes), were phenotyped for their fruit stripe pattern. The CTAB 2X protocol was used for DNA extraction and 116 microsatellite markers were examined in a group of F2 plants that had fruit with well-defined stripes and fruit with diffuse stripes. The microsatellite loci MCPI_05 and MCPI_16 exhibited a linkage to the stripe pattern at a distance of 1.5 and 1.8 cM, respectively, with LOD scores of 39.28 and 38.11, respectively, which were located on chromosome six of the watermelon genome. These markers can be used in marker-assisted selection in watermelon improvement programs, by various research institutions.
650 $aCitrullus Lanatus
650 $aMelancia
653 $aFruit external pattern
653 $aMarcadores microssatélites
653 $aMelhoramento genético
653 $aWatermelon
700 1 $aSANTOS, C. A. F.
700 1 $aDIAS, R. de C. S.
700 1 $aALVES, J. C. S. F.
700 1 $aNOGUEIRA, T. O.
773 $tGenetics and Molecular Research$gv. 14, n. 1, p. 269-276, 2015.
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