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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
20/01/2015 |
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Data da última atualização: |
08/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CRATIVOL, C.; REGULSKI, M.; BERTALAN, M.; MCCOMBIE, W. R.; SILVA, F. R. da; ZERLOTINI NETO, A.; VICENTINI, R.; FARINELLI, L.; HEMERLY, A. S.; MARTIENSSEN, R. A.; FERREIRA, P. C. G. |
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Afiliação: |
CLÍCIA GRATIVOL, UFRJ; MICHAEL REGULSKI, Cold Spring Harbor Laboratory; MARCELO BERTALAN, Institute of Biological Psychiatry, Mental Health Center; W. RICHARD MCCOMBIE, Cold Spring Harbor Laboratory; FELIPE RODRIGUES DA SILVA, CNPTIA; ADHEMAR ZERLOTINI NETO, CNPTIA; RENATO VICENTINI, CBMEG/Unicamp; LAURENT FARINELLI, Fasteris SA; ADRIANA SILVA HEMERLY, UFRJ; ROBERT A. MARTIENSSEN, Cold Spring Harbor Laboratory; PAULO CAVALCANTI GOMES FERREIRA, UFRJ. |
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Título: |
Sugarcane genome sequencing by methylation filtration provides tools for genomic research in the genus Saccharum. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
The Plant Journal, Oxford, v. 79, n. 1, p. 162-172, 2014. |
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DOI: |
10.1111/tpj.12539 |
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Idioma: |
Inglês |
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Conteúdo: |
Many economically important crops have large and complex genomes that hamper their sequencing by standard methods such as whole genome shotgun (WGS). Large tracts of methylated repeats occur in plant genomes that are interspersed by hypomethylated gene-rich regions. Gene-enrichment strategies based on methylation profiles offer an alternative to sequencing repetitive genomes. Here, we have applied methyl filtration with McrBC endonuclease digestion to enrich for euchromatic regions in the sugarcane genome. To verify the efficiency of methylation filtration and the assembly quality of sequences submitted to gene-enrichment strategy, we have compared assemblies using methyl-filtered (MF) and unfiltered (UF) libraries. The use of methy filtration allowed a better assembly by filtering out 35% of the sugarcane genome and by producing 1.53 more scaffolds and 1.73 more assembled Mb in length compared with unfiltered dataset. The coverage of sorghum coding sequences (CDS) by MF scaffolds was at least 36% higher than by the use of UF scaffolds. Using MF technology, we increased by 1343 the coverage of gene regions of the monoploid sugarcane genome. The MF reads assembled into scaffolds that covered all genes of the sugarcane bacterial artificial chromosomes (BACs), 97.2% of sugarcane expressed sequence tags (ESTs), 92.7% of sugarcane RNA-seq reads and 98.4% of sorghum protein sequences. Analysis of MF scaffolds from encoded enzymes of the sucrose/starch pathway discovered 291 single-nucleotide polymorphisms (SNPs) in the wild sugarcane species, S. spontaneum and S. officinarum. A large number of microRNA genes was also identified in the MF scaffolds. The information achieved by the MF dataset provides a valuable tool for genomic research in the genus Saccharum and for improvement of sugarcane as a biofuel crop. MenosMany economically important crops have large and complex genomes that hamper their sequencing by standard methods such as whole genome shotgun (WGS). Large tracts of methylated repeats occur in plant genomes that are interspersed by hypomethylated gene-rich regions. Gene-enrichment strategies based on methylation profiles offer an alternative to sequencing repetitive genomes. Here, we have applied methyl filtration with McrBC endonuclease digestion to enrich for euchromatic regions in the sugarcane genome. To verify the efficiency of methylation filtration and the assembly quality of sequences submitted to gene-enrichment strategy, we have compared assemblies using methyl-filtered (MF) and unfiltered (UF) libraries. The use of methy filtration allowed a better assembly by filtering out 35% of the sugarcane genome and by producing 1.53 more scaffolds and 1.73 more assembled Mb in length compared with unfiltered dataset. The coverage of sorghum coding sequences (CDS) by MF scaffolds was at least 36% higher than by the use of UF scaffolds. Using MF technology, we increased by 1343 the coverage of gene regions of the monoploid sugarcane genome. The MF reads assembled into scaffolds that covered all genes of the sugarcane bacterial artificial chromosomes (BACs), 97.2% of sugarcane expressed sequence tags (ESTs), 92.7% of sugarcane RNA-seq reads and 98.4% of sorghum protein sequences. Analysis of MF scaffolds from encoded enzymes of the sucrose/starch pathway discovered 291 single... Mostrar Tudo |
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Palavras-Chave: |
Cana-de-açúcar; De novo assembly; Gene-rich regions. |
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Thesagro: |
Metilação. |
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Thesaurus Nal: |
Methylation; Saccharum; Sugarcane. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02815naa a2200337 a 4500
001 2006128
005 2020-01-08
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1111/tpj.12539$2DOI
100 1 $aCRATIVOL, C.
245 $aSugarcane genome sequencing by methylation filtration provides tools for genomic research in the genus Saccharum.$h[electronic resource]
260 $c2014
520 $aMany economically important crops have large and complex genomes that hamper their sequencing by standard methods such as whole genome shotgun (WGS). Large tracts of methylated repeats occur in plant genomes that are interspersed by hypomethylated gene-rich regions. Gene-enrichment strategies based on methylation profiles offer an alternative to sequencing repetitive genomes. Here, we have applied methyl filtration with McrBC endonuclease digestion to enrich for euchromatic regions in the sugarcane genome. To verify the efficiency of methylation filtration and the assembly quality of sequences submitted to gene-enrichment strategy, we have compared assemblies using methyl-filtered (MF) and unfiltered (UF) libraries. The use of methy filtration allowed a better assembly by filtering out 35% of the sugarcane genome and by producing 1.53 more scaffolds and 1.73 more assembled Mb in length compared with unfiltered dataset. The coverage of sorghum coding sequences (CDS) by MF scaffolds was at least 36% higher than by the use of UF scaffolds. Using MF technology, we increased by 1343 the coverage of gene regions of the monoploid sugarcane genome. The MF reads assembled into scaffolds that covered all genes of the sugarcane bacterial artificial chromosomes (BACs), 97.2% of sugarcane expressed sequence tags (ESTs), 92.7% of sugarcane RNA-seq reads and 98.4% of sorghum protein sequences. Analysis of MF scaffolds from encoded enzymes of the sucrose/starch pathway discovered 291 single-nucleotide polymorphisms (SNPs) in the wild sugarcane species, S. spontaneum and S. officinarum. A large number of microRNA genes was also identified in the MF scaffolds. The information achieved by the MF dataset provides a valuable tool for genomic research in the genus Saccharum and for improvement of sugarcane as a biofuel crop.
650 $aMethylation
650 $aSaccharum
650 $aSugarcane
650 $aMetilação
653 $aCana-de-açúcar
653 $aDe novo assembly
653 $aGene-rich regions
700 1 $aREGULSKI, M.
700 1 $aBERTALAN, M.
700 1 $aMCCOMBIE, W. R.
700 1 $aSILVA, F. R. da
700 1 $aZERLOTINI NETO, A.
700 1 $aVICENTINI, R.
700 1 $aFARINELLI, L.
700 1 $aHEMERLY, A. S.
700 1 $aMARTIENSSEN, R. A.
700 1 $aFERREIRA, P. C. G.
773 $tThe Plant Journal, Oxford$gv. 79, n. 1, p. 162-172, 2014.
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Embrapa Agricultura Digital (CNPTIA) |
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| 1. |  | AVILA, P. C.; CANUTO, J. C.; GALLAR HERNANDEZ, D. Aplicação de uma proposta metodológica de Diagnóstico Participativo da Vulnerabilidade à Insegurança Alimentar a nível local em quatro comunidades rurais do Vale de Cusco, Peru. Cadernos de Agroecologia, Porto Alegre, v. 13, n. 1, p. 1-6, Jul. 2018. Edição dos anais do VI Congresso Latino-Americano (CLAA), X Congresso Brasileiro (CBA), V Seminário do DF e Entorno (SEMDF), 12-15 setembro de 2017, Brasília, DF, Brasil.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 4. | | AVILA, P. C.; JUNQUEIRA, A. da C.; GONCALVES, J. R. P.; MORICONI, W.; CANUTO, J. C. Avaliação da resistência à penetração em diferentes sistemas em lote de reforma agrária no município de Itapeva, SP. In: JORNADA DE ESTUDOS EM ASSENTAMENTOS RURAIS, 6., 2013, Campinas. Caderno de Resumos... Campinas: Universidade Estadual de Campinas, 2013. 6 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 5. | | CANUTO, J. C.; CAMARGO, R. C. R. de; URCHEI, M. A.; AVILA, P. C. Os sistemas agroflorestais biodiversos na perspectiva da segurança alimentar. In: MAGNONI JÚNIOR, L.; STEVENS, D.; SILVA, W. T. L. da; VALE, J. M. F. do; PURINI, S. R. de M.; MAGNONI, M. da G. M.; SEBASTIÃO, E.; BRANCO JÚNIOR, G.; ADORNO FILHO, E. F.; FIGUEIREDO, W. dos S.; SEBASTIÃO, I. (Org.). JC na Escola Ciência, Tecnologia e Sociedade: mobilizar o conhecimento para alimentar o Brasil. 2. ed. São Paulo: Centro Paula Souza, 2017. p. 373-389.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 7. | | QUEIROGA, J. L. de; JUNQUEIRA, A. da C.; AVILA, P. C.; CANUTO, J. C.; URCHEI, M. A.; MALAGODI-BRAGA, K. S. Monitoramento em agroflorestas: adequação de metodologias para a avaliação da sustentabilidade. Cadernos de Agroecologia, Cruz Alta, v. 8, n. 2, 2013. Resumo 15155. Edição dos resumos do VIII Congresso Brasileiro de Agroecologia, Porto Alegre, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 7 | |
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