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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
19/11/2014 |
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Data da última atualização: |
19/11/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VARELA, A. P. M.; SANTOS, H. F. dos; CIBULSKI, S. P.; SCHEFFER, C. M.; SCHMIDT, C.; LIMA, F. E. S.; SILVA, A. D.; ESTEVES, P. A.; FRANCO, A. C.; ROEHE, P. M. |
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Afiliação: |
ANA PAULA MUTERLE VARELA, FEPAGRO; HELTON FERNANDES DOS SANTOS, FEPAGRO; SAMUEL PAULO CIBULSKI, FEPAGRO; CAMILA MENGUE SCHEFFER, FEPAGRO; CANDICE SCHMIDT, FEPAGRO; FRANCISCO ESMAILE SALES LIMA, UFGRS; ALESSANDRA D'ÁVILA SILVA; PAULO AUGUSTO ESTEVES, CNPSA; ANA CLÁUDIA FRANCO, UFGRS; PAULO MICHEL ROEHE, FEPAGRO. |
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Título: |
Chicken anemia virus and avian gyrovirus 2 as contaminants in poultry vaccines. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Biologicals, London, v. 42, n. 6., p. 346–350, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
This study focuses on the detection of chicken anemia virus (CAV) and avian gyrovirus 2 (AGV2) genomes in commercially available poultry vaccines. A duplex quantitative real-time PCR (dqPCR), capable of identifying genomes of both viruses in a single assay, was employed to determine the viral loads of these agents in commercially available vaccines. Thirty five vaccines from eight manufacturers (32 prepared with live and 3 with inactivated microorganisms) were examined. Genomes of CAV were detected as contaminants in 6/32 live vaccines and in 1/3 inactivated vaccines. The CAV genome loads ranged from 6.4 to 173.4 per 50 ng of vaccine DNA (equivalent to 0.07 to 0.69 genome copies per dose of vaccine). Likewise, AGV2 genomes were detected in 9/32 live vaccines, with viral loads ranging from 93 to 156,187 per 50 ng of vaccine DNA (equivalent to 0.28e9176 genome copies per dose of vaccine). These findings provide evidence for the possibility of contamination of poultry vaccines with CAV and AGV2 and they also emphasize the need of searching for these agents in vaccines in order to ensure the absence of such potential contaminants. |
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Thesagro: |
Anemia infecciosa; Ave doméstica; Frango; Virologia. |
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Thesaurus Nal: |
Chicken anemia virus; Gyrovirus; Live vaccines; Poultry; Virology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02059naa a2200337 a 4500
001 2000508
005 2014-11-19
008 2014 bl uuuu u00u1 u #d
100 1 $aVARELA, A. P. M.
245 $aChicken anemia virus and avian gyrovirus 2 as contaminants in poultry vaccines.$h[electronic resource]
260 $c2014
520 $aThis study focuses on the detection of chicken anemia virus (CAV) and avian gyrovirus 2 (AGV2) genomes in commercially available poultry vaccines. A duplex quantitative real-time PCR (dqPCR), capable of identifying genomes of both viruses in a single assay, was employed to determine the viral loads of these agents in commercially available vaccines. Thirty five vaccines from eight manufacturers (32 prepared with live and 3 with inactivated microorganisms) were examined. Genomes of CAV were detected as contaminants in 6/32 live vaccines and in 1/3 inactivated vaccines. The CAV genome loads ranged from 6.4 to 173.4 per 50 ng of vaccine DNA (equivalent to 0.07 to 0.69 genome copies per dose of vaccine). Likewise, AGV2 genomes were detected in 9/32 live vaccines, with viral loads ranging from 93 to 156,187 per 50 ng of vaccine DNA (equivalent to 0.28e9176 genome copies per dose of vaccine). These findings provide evidence for the possibility of contamination of poultry vaccines with CAV and AGV2 and they also emphasize the need of searching for these agents in vaccines in order to ensure the absence of such potential contaminants.
650 $aChicken anemia virus
650 $aGyrovirus
650 $aLive vaccines
650 $aPoultry
650 $aVirology
650 $aAnemia infecciosa
650 $aAve doméstica
650 $aFrango
650 $aVirologia
700 1 $aSANTOS, H. F. dos
700 1 $aCIBULSKI, S. P.
700 1 $aSCHEFFER, C. M.
700 1 $aSCHMIDT, C.
700 1 $aLIMA, F. E. S.
700 1 $aSILVA, A. D.
700 1 $aESTEVES, P. A.
700 1 $aFRANCO, A. C.
700 1 $aROEHE, P. M.
773 $tBiologicals, London$gv. 42, n. 6., p. 346–350, 2014.
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