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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
07/02/2017 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, L. F. da; GUERRA, C. C.; KLEIN, D.; BERGOLD, A. M. |
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Afiliação: |
LETICIA FLORES DA SILVA, CNPUV; CELITO CRIVELLARO GUERRA, CNPUV; Diandra Klein, Faculdade de Farmácia, Universidade de Caxias do Sul; Ana Maria Bergold, Laboratório de Química Farmacêutica, Universidade Federal do Rio Grande do Sul. |
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Título: |
Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Food Chemistryl, v. 227, p. 158-165, 2017. |
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DOI: |
http://dx.doi.org/10.1016/j.foodchem.2017.01.087 |
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Idioma: |
Inglês |
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Conteúdo: |
Bioactive phenols (BPs) are often targets in red wine analysis. However, other compounds interfere in the liquid chromatography methods used for this analysis. Here, purification procedures were tested to eliminate anthocyanin interference during the determination of 19 red-wine BPs. Liquid chromatography, coupled to a diode array detector (HPLC-DAD) and a mass spectrometer (UPLC-MS), was used to compare the direct injection of the samples with solid-phase extractions: reversed-phase (C18) and strong cation-exchange (SCX). The HPLC-DAD method revealed that, out of 13 BPs, only six are selectively analyzed with or without C18 treatment, whereas SCX enabled the detection of all BPs. The recovery with SCX was above 86.6% for eight BPs. Moreover, UPLC-MS demonstrated the potential of SCX sample preparation for the determination of 19 BPs. The developed procedure may be extended to the analysis of other red wine molecules or to other analytical methods where anthocyanins may interfere. Chemical compounds studied in this article trans-Resveratrol (PubChemCID: 445154); trans-?-Viniferin (PubChemCID: 5315233); Quercetin (PubChemCID: 5280343); Myricetin (PubChemCID: 5281672); Kaempferol (PubChemCID: 5280863); trans-Cinnamic acid (PubChemCID: 444539); trans-Piceid (PubChemCID: 5281718); Taxifolin (PubChemCID: 439533) Keywords Bioactive phenols; Red wine; Liquid chromatography; Mass spectrometry; Diode array detector; Sample purification |
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Palavras-Chave: |
Bioactive phenols; Diode array detector; Mass spectometry; Red wine; Sample purification. |
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Thesaurus Nal: |
Liquid Chromatography. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154893/1/Published-version-2017.pdf
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Marc: |
LEADER 02219naa a2200241 a 4500
001 2062946
005 2019-05-06
008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.foodchem.2017.01.087$2DOI
100 1 $aSILVA, L. F. da
245 $aSolid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.$h[electronic resource]
260 $c2017
520 $aBioactive phenols (BPs) are often targets in red wine analysis. However, other compounds interfere in the liquid chromatography methods used for this analysis. Here, purification procedures were tested to eliminate anthocyanin interference during the determination of 19 red-wine BPs. Liquid chromatography, coupled to a diode array detector (HPLC-DAD) and a mass spectrometer (UPLC-MS), was used to compare the direct injection of the samples with solid-phase extractions: reversed-phase (C18) and strong cation-exchange (SCX). The HPLC-DAD method revealed that, out of 13 BPs, only six are selectively analyzed with or without C18 treatment, whereas SCX enabled the detection of all BPs. The recovery with SCX was above 86.6% for eight BPs. Moreover, UPLC-MS demonstrated the potential of SCX sample preparation for the determination of 19 BPs. The developed procedure may be extended to the analysis of other red wine molecules or to other analytical methods where anthocyanins may interfere. Chemical compounds studied in this article trans-Resveratrol (PubChemCID: 445154); trans-?-Viniferin (PubChemCID: 5315233); Quercetin (PubChemCID: 5280343); Myricetin (PubChemCID: 5281672); Kaempferol (PubChemCID: 5280863); trans-Cinnamic acid (PubChemCID: 444539); trans-Piceid (PubChemCID: 5281718); Taxifolin (PubChemCID: 439533) Keywords Bioactive phenols; Red wine; Liquid chromatography; Mass spectrometry; Diode array detector; Sample purification
650 $aLiquid Chromatography
653 $aBioactive phenols
653 $aDiode array detector
653 $aMass spectometry
653 $aRed wine
653 $aSample purification
700 1 $aGUERRA, C. C.
700 1 $aKLEIN, D.
700 1 $aBERGOLD, A. M.
773 $tFood Chemistryl$gv. 227, p. 158-165, 2017.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/07/2019 |
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Data da última atualização: |
20/08/2019 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA. |
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Título: |
Relatório de destaques 2018: preservando o passado e antecipando o futuro. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Brasília, DF: 2019. |
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Páginas: |
120 p. |
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Série: |
(Embrapa Recursos Genéticos e Biotecnologia / Documentos, 364) |
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Idioma: |
Português |
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Palavras-Chave: |
Relatório; Tecnologia e inovação. |
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Thesagro: |
Agricultura; Gestão; Instituição de Pesquisa; Pesquisa Agrícola. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/199805/1/Relatorio-de-destaques-2018-2.pdf
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Marc: |
LEADER 00640nam a2200193 a 4500
001 2110810
005 2019-08-20
008 2019 bl uuuu 00u1 u #d
100 1 $aEMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA.
245 $aRelatório de destaques 2018$bpreservando o passado e antecipando o futuro.$h[electronic resource]
260 $aBrasília, DF: 2019.$c2019
300 $a120 p.
490 $a(Embrapa Recursos Genéticos e Biotecnologia / Documentos, 364)
650 $aAgricultura
650 $aGestão
650 $aInstituição de Pesquisa
650 $aPesquisa Agrícola
653 $aRelatório
653 $aTecnologia e inovação
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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