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| Registros recuperados : 7 | |
| 1. |  | FARIA, A. R. N.; NORONHA, A. C. da S.; OLIVEIRA, A. A. R.; OLIVEIRA, A. M. G.; CARDOSO, C. E. L.; RITZINGER, C. H. S. P.; OLIVEIRA, E. J. de; COELHO, E. F.; SANTOS FILHO, H. P.; CRUZ, J. L.; OLIVEIRA, J. R. P.; DANTAS, J. L. L.; SOUZA, L. D.; OLIVEIRA, M. de A.; COELHO FILHO, M. A.; SANCHES, N. F.; MEISSNER FILHO, P. E.; MEDINA, V. M.; CORDEIRO, Z. J. M. A cultura do mamão. 3. ed. rev. e ampl. Brasília, DF: Embrapa Informação Tecnológica; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 119 p. il. (Coleção plantar, 65). Autoria: COROLEIRO (i.e. CORDEIRO), Z. J. M.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Pantanal; Embrapa Unidades Centrais. |
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| 2. | | FARIA, A. R. N.; NORONHA, A. C. da S.; OLIVEIRA, A A. R.; OLIVEIRA, A. M. G.; CARDOSO, C. E. L.; RITZINGER, C. H. S. P.; OLIVEIRA, E. J. de; COELHO, E. F.; SANTOS FILHO, H. P.; CRUZ, J. L.; OLIVEIRA, J. R. P. de; DANTAS, J. L. L.; SOUZA, L. D.; OLIVEIRA, M. de A.; COELHO FILHO, M. A.; SANCHES, N. F.; MEISSNER FILHO, P. E.; MEDINA, V. M.; COROLEIRO, Z. J. M. A cultura do mamão. 3. ed. rev. e ampl. Brasília, DF: Embrapa Informação Tecnológica; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 119 p. il. (Coleção plantar, 65). Autoria: COROLEIRO (i.e. CORDEIRO), Z. J. M.| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Cerrados; Embrapa Instrumentação; Embrapa Mandioca e Fruticultura; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia; Embrapa Roraima; Embrapa Semiárido; Embrapa Solos / UEP-Recife. MenosEmbrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Cerrados; Embrapa Instrumentação; Embrapa Mandioca e Fruticultura; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia... Mostrar Todas |
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| 4. | | FARIA, A. R. N.; NORONHA, A. C. da S.; OLIVEIRA, A A. R.; OLIVEIRA, A. M. G.; CARDOSO, C. E. L.; RITZINGER, C. H. S. P.; OLIVEIRA, E. J. de; COELHO, E. F.; SANTOS FILHO, H. P.; CRUZ, J. L.; OLIVEIRA, J. R. P. de; DANTAS, J. L. L.; SOUZA, L. D.; OLIVEIRA, M. de A.; COELHO FILHO, M. A.; SANCHES, N. F.; MEISSNER FILHO, P. E.; MEDINA, V. M.; COROLEIRO, Z. J. M. A cultura do mamão. 3. ed. rev. e ampl. Brasília, DF: Embrapa Informação Tecnológica; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 119 p. il. (Coleção plantar, 65). Autoria: COROLEIRO (i.e. CORDEIRO), Z. J. M.| Biblioteca(s): Embrapa Clima Temperado. |
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| 6. | | FARIAS, A. R. N.; OLIVEIRA, A. M. G.; SANTOS FILHO, H. P.; OLIVEIRA, J. R. P. de; DANTAS, J. L. L.; OLIVEIRA, M. de A.; SANCHES, N. F.; MEDINA, V. M.; CORDEIRO, Z. J. M. A cultura do mamão. 2. ed. rev. e aum. Brasília, DF: Embrapa-SPI; Cruz das Almas: Embrapa-CNPMF, 1998. 95 p. (Coleção plantar, 37).| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Instrumentação; Embrapa Mandioca e Fruticultura; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia; Embrapa Semiárido; Embrapa Soja; Embrapa Tabuleiros Costeiros; Embrapa Unidades Centrais. MenosEmbrapa Agroindústria de Alimentos; Embrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Instrumentação; Embrapa Mandioca e Fruticultura; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte; Embrapa Pecuária Sul... Mostrar Todas |
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| Registros recuperados : 7 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
10/02/2014 |
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Data da última atualização: |
08/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MOURÃO, M. de M.; BITAR, M.; LOBO, F. P.; PECONICK, A. P.; GRYNBERG, P.; PROSDOCIMI, F.; WAISBERG, M.; CERQUEIRA, G. C.; MACEDO, A. M.; MACHADO, C. R.; YOSHINO, T.; FRANCO, G. R. |
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Afiliação: |
MARINA DE MORAES MOURÃO, Centro de Pesquisas René Rachou-Fiocruz; MAINÁ BITAR, UFMG; FRANCISCO PEREIRA LOBO, CNPTIA; ANA PAULA PECONICK, Ufla; PRISCILA GRYNBERG, UFMG; FRANCISCO PROSDOCIMI, UFRJ; MICHAEL WAISBERG, University of Virginia; GUSTAVO COUTINHO CERQUEIRA, Broad Institute of Massachusetts Institute of Technology and Harvard; ANDRÉA MARA MACEDO, UFMG; CARLOS RENATO MACHADO, UFMG; TIMOTHY YOSHINO, University of Wisconsin; GLÓRIA REGINA FRANCO, UFMG. |
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Título: |
A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974). MenosSchistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different librari... Mostrar Tudo |
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Palavras-Chave: |
Interferência de RNA. |
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Thesagro: |
Schistosoma Mansoni. |
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Thesaurus NAL: |
RNA interference; Trans-splicing. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02851naa a2200301 a 4500
001 1979292
005 2020-01-08
008 2013 bl uuuu u00u1 u #d
100 1 $aMOURÃO, M. de M.
245 $aA directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni.$h[electronic resource]
260 $c2013
520 $aSchistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974).
650 $aRNA interference
650 $aTrans-splicing
650 $aSchistosoma Mansoni
653 $aInterferência de RNA
700 1 $aBITAR, M.
700 1 $aLOBO, F. P.
700 1 $aPECONICK, A. P.
700 1 $aGRYNBERG, P.
700 1 $aPROSDOCIMI, F.
700 1 $aWAISBERG, M.
700 1 $aCERQUEIRA, G. C.
700 1 $aMACEDO, A. M.
700 1 $aMACHADO, C. R.
700 1 $aYOSHINO, T.
700 1 $aFRANCO, G. R.
773 $tMemórias do Instituto Oswaldo Cruz, Rio de Janeiro$gv. 108, n. 6, p. 707-717, Sept. 2013.
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