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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
08/08/2011 |
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Data da última atualização: |
08/08/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RAMOS, C. A. N.; ARAUJO, F. R.; SOUZA, I. I. F.; BACANELLI, G.; LUIZ, H. L.; RUSSI, L. S.; OLIVEIRA, R. H. M. de; SOARES, C. O.; ROSINHA, G. M. S.; ALVES, L. C. |
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Afiliação: |
Carlos Alberto do Nascimento Ramos, Bolsista; FLABIO RIBEIRO ARAUJO, CNPGC; Ingrid I.F. Souza, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco; G. Bacanelli, BOLSISTA; Hera L. Luiz, Bolsista; Lívia S. Russi, Bolsista; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC; Leucio C. Alves, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco. |
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Título: |
Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Veterinary Parasitology, v.176, p.79-83, Feb. 2011. Issue 1. |
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Idioma: |
Inglês |
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Conteúdo: |
This paper reports a quantitative real-time polymerase chain reaction (q-PCR) based on the msa2c gene and standardized with Platinum SYBR Green/ROX for the detection of Babesia bovis in cattle. The msa2c q-PCR amplified a DNA fragment with average dissociation temperature of 77.41 ◦C (±0.25 ◦C). No amplification was detected when DNA from B. bigemina, A. marginale or Bos taurus was used as the template. The detection limit of the msa2c q-PCR was 1000 copies per ml of blood sample, with a linear correlation between the number of msa2c copies and threshold cycle. The comparison between msa2c q-PCR and conventional PCR for cytochrome b revealed 88.8% agreement, with a Kappa index of 0.75. In the comparison between msa2c q-PCR and an enzyme-linked immunosorbent assay (ELISA) with semi-purified B. bovis antigen, agreement was 96.3% and the Kappa index was 0.91. The agreement between three tests was 85.8%. The msa2c q-PCR detected a higher number of positive cattle than conventional PCR in an enzootically stable area, but did not differ significantly from ELISA. No significant differences were detected between the three diagnostic tests with cattle from an enzootically unstable area. All animals raised on a tick-free facility were negative for B. bovis in the three tests. These results suggest that msa2c q-PCR is a useful test for the detection of B. bovis infection. |
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Thesagro: |
Babesia Bovis; Babesiose; Sanidade Animal. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02160naa a2200265 a 4500
001 1897432
005 2011-08-08
008 2011 bl uuuu u00u1 u #d
100 1 $aRAMOS, C. A. N.
245 $aReal-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.$h[electronic resource]
260 $c2011
520 $aThis paper reports a quantitative real-time polymerase chain reaction (q-PCR) based on the msa2c gene and standardized with Platinum SYBR Green/ROX for the detection of Babesia bovis in cattle. The msa2c q-PCR amplified a DNA fragment with average dissociation temperature of 77.41 ◦C (±0.25 ◦C). No amplification was detected when DNA from B. bigemina, A. marginale or Bos taurus was used as the template. The detection limit of the msa2c q-PCR was 1000 copies per ml of blood sample, with a linear correlation between the number of msa2c copies and threshold cycle. The comparison between msa2c q-PCR and conventional PCR for cytochrome b revealed 88.8% agreement, with a Kappa index of 0.75. In the comparison between msa2c q-PCR and an enzyme-linked immunosorbent assay (ELISA) with semi-purified B. bovis antigen, agreement was 96.3% and the Kappa index was 0.91. The agreement between three tests was 85.8%. The msa2c q-PCR detected a higher number of positive cattle than conventional PCR in an enzootically stable area, but did not differ significantly from ELISA. No significant differences were detected between the three diagnostic tests with cattle from an enzootically unstable area. All animals raised on a tick-free facility were negative for B. bovis in the three tests. These results suggest that msa2c q-PCR is a useful test for the detection of B. bovis infection.
650 $aBabesia Bovis
650 $aBabesiose
650 $aSanidade Animal
700 1 $aARAUJO, F. R.
700 1 $aSOUZA, I. I. F.
700 1 $aBACANELLI, G.
700 1 $aLUIZ, H. L.
700 1 $aRUSSI, L. S.
700 1 $aOLIVEIRA, R. H. M. de
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
700 1 $aALVES, L. C.
773 $tVeterinary Parasitology$gv.176, p.79-83, Feb. 2011. Issue 1.
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