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Registro Completo |
Biblioteca(s): |
Embrapa Acre. |
Data corrente: |
15/02/2012 |
Data da última atualização: |
16/11/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
AZEVEDO, J. M. A. de; ASSIS, G. M. L. de; SILVA, H. S. F. da; SANTOS, E. C. dos; MENDONÇA, M. da S. de; MARCOLINO, E. F. |
Afiliação: |
JOSÉ MARLO DE ARAÚJO AZEVEDO, BOLSISTA CNPq; GISELLE MARIANO LESSA DE ASSIS, CPAF-AC; HELLEN SANDRA FREIRES DA SILVA, BOLSISTA CNPq; ERLAÍLSON COSTA DOS SANTOS, UFAC; MÁRCIA DA SILVA DE MENDONÇA, UNINORTE; EDIRLEI FROTA MARCOLINO, UNINORTE. |
Título: |
Comprimento do maior estolão em genótipos de amendoim forrageiro submetidos à inoculação micorrízica e adubação fosfatada. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém, PA. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém, PA: SBZ, 2011. |
Páginas: |
3 p. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Conteúdo: |
O amendoim forrageiro produz uma camada densa de estolões e é capaz de nodular e fixar nitrogênio em simbiose com FMAs. O objetivo deste estudo foi determinar o comprimento do maior estolão em genótipos de amendoim forrageiro submetidos à inoculação micorrízica e adubação fosfatada. |
Palavras-Chave: |
Adubação fosfatada; Amendoim forrageiro; Fungos micorrízicos. |
Thesaurus Nal: |
Arachis pintoi. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54171/1/21122.pdf
|
Marc: |
LEADER 01195nam a2200229 a 4500 001 1915656 005 2023-11-16 008 2011 bl uuuu u00u1 u #d 100 1 $aAZEVEDO, J. M. A. de 245 $aComprimento do maior estolão em genótipos de amendoim forrageiro submetidos à inoculação micorrízica e adubação fosfatada.$h[electronic resource] 260 $aREUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém, PA. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém, PA: SBZ$c2011 300 $a3 p.$c1 CD-ROM. 520 $aO amendoim forrageiro produz uma camada densa de estolões e é capaz de nodular e fixar nitrogênio em simbiose com FMAs. O objetivo deste estudo foi determinar o comprimento do maior estolão em genótipos de amendoim forrageiro submetidos à inoculação micorrízica e adubação fosfatada. 650 $aArachis pintoi 653 $aAdubação fosfatada 653 $aAmendoim forrageiro 653 $aFungos micorrízicos 700 1 $aASSIS, G. M. L. de 700 1 $aSILVA, H. S. F. da 700 1 $aSANTOS, E. C. dos 700 1 $aMENDONÇA, M. da S. de 700 1 $aMARCOLINO, E. F.
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
23/08/2012 |
Data da última atualização: |
22/02/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; MELO, D. S. de; NICIURA, S. C. M.; GARCIA, J. M. |
Afiliação: |
NAIARA ZOCCAL SARAIVA, Universidade Estadual Paulista; CLARA SLADE OLIVEIRA, UNESP; TATIANE ALMEIDA DRUMMOND TETZNER, UNESP; MARINA RAGAGNIN DE LIMA, UNESP/JABOTICABAL; DANILAS SALINET DE MELO, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
Título: |
Chemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Cellular Reprogramming, v. 14, n. 5, p. 1-11, 2012. |
DOI: |
10.1089/cell.2011.0077 |
Idioma: |
Inglês |
Conteúdo: |
Despite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)?derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Where as in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher ( p < 0.05) levels of G6PD were observed in SCNT deme and in vitro?derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher metabolism in embryos considered of superior quality or if the presence of higher reactive oxygen species (ROS) levels generated by the increased oxygen consumption triggers G6PD activation, the expression of genes related to stress response should be investigated in embryos produced by that technique. MenosDespite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)?derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Where as in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher ( p < 0.05) levels of G6PD were observed in SCNT deme and in vitro?derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher m... Mostrar Tudo |
Palavras-Chave: |
Higher G6PD; Nuclear transfer; Somatic cell. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02567naa a2200241 a 4500 001 1932049 005 2023-02-22 008 2012 bl uuuu u00u1 u #d 024 7 $a10.1089/cell.2011.0077$2DOI 100 1 $aSARAIVA, N. Z. 245 $aChemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer.$h[electronic resource] 260 $c2012 520 $aDespite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)?derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Where as in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher ( p < 0.05) levels of G6PD were observed in SCNT deme and in vitro?derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher metabolism in embryos considered of superior quality or if the presence of higher reactive oxygen species (ROS) levels generated by the increased oxygen consumption triggers G6PD activation, the expression of genes related to stress response should be investigated in embryos produced by that technique. 653 $aHigher G6PD 653 $aNuclear transfer 653 $aSomatic cell 700 1 $aOLIVEIRA, C. S. 700 1 $aTETZNER, T. A. D. 700 1 $aLIMA, M. R. de 700 1 $aMELO, D. S. de 700 1 $aNICIURA, S. C. M. 700 1 $aGARCIA, J. M. 773 $tCellular Reprogramming$gv. 14, n. 5, p. 1-11, 2012.
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