Data/hora: 19/06/2024 05:18:58

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	18/09/1995	
Data da última atualização: 	12/04/2023	
Autoria: 	DORSEY, T. E.; McDONALD, P. W.; ROELS, O. A.	
Afiliação: 	University of Texas Marine Institute, Port Aransas Laboratory, Port Aransas, Texas.	
Título: 	A heated biuret-folin protein assay which gives equal absorbance with different proteins.	
Ano de publicação: 	1977	
Fonte/Imprenta: 	Analytical Biochemistry, v.78, p.156-164, 1977.	
Idioma: 	Inglês	
Conteúdo: 	This protein assay requires heating the sample with biuret reagent for 100 min at 100grausC before addition of Folin. Different proteins give almost identical optical densities on a nitrogen basis with this procedure. The absorbance per microgram-atom of protein nitrogin is linear at or below 0.400. A convenient final concentration of protein in this assay is about 0.2 microg-atom of protein nitrogen per ml. Most of the absorbance is due to amino acid combinations other than tyrosine and tryptophan in the protein. The specificity appears similar to that of the biuret where few compounds of biological importance interfere significantly in the assay at normal cellular concentrations.	
Palavras-Chave: 	Bioquimical Nitrogenio.	
Thesagro: 	Proteína.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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