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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
30/12/2019 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PILLAI, V. V.; SIQUEIRA, L. G. B.; DAS, M.; KEI, T. G.; TU, L. N.; HERREN, A. W.; PHINNEY, B. S.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V. |
Afiliação: |
VIJU VIJAYAN PILLAI, Cornell University, Ithaca, NY; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MOUBANI DAS, Cornell University, Ithaca, NY; TIFFANY G. KEI, Cornell University, Ithaca, NY; LAN N. TU, Cornell University, Ithaca, NY; ANTHONY W. HERREN, University of California; BRETT S. PHINNEY, University of California; SOON HON CHEONG, Cornell University, Ithaca, NY; PETER J. HANSEN, University of Florida, Gainesville, FL; VIMAL SELVARAJ, Cornell University, Ithaca, NY. |
Título: |
Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Biology Open, v. 8, n. 5, article bio037937, 2019. |
DOI: |
https://doi.org/10.1242/bio.037937 |
Idioma: |
Inglês |
Conteúdo: |
Trophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation. MenosTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understandi... Mostrar Tudo |
Thesagro: |
Placenta. |
Thesaurus Nal: |
Blastocyst; Embryo implantation; Pregnancy; Stem cells; Trophoblast. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207915/1/BiolOpen-LGustavoSiq-Physiological-bio037937.full.pdf
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Marc: |
LEADER 02435naa a2200313 a 4500 001 2117829 005 2024-02-06 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1242/bio.037937$2DOI 100 1 $aPILLAI, V. V. 245 $aPhysiological profile of undifferentiated bovine blastocyst-derived trophoblasts.$h[electronic resource] 260 $c2019 520 $aTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation. 650 $aBlastocyst 650 $aEmbryo implantation 650 $aPregnancy 650 $aStem cells 650 $aTrophoblast 650 $aPlacenta 700 1 $aSIQUEIRA, L. G. B. 700 1 $aDAS, M. 700 1 $aKEI, T. G. 700 1 $aTU, L. N. 700 1 $aHERREN, A. W. 700 1 $aPHINNEY, B. S. 700 1 $aCHEONG, S. H. 700 1 $aHANSEN, P. J. 700 1 $aSELVARAJ, V. 773 $tBiology Open$gv. 8, n. 5, article bio037937, 2019.
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Embrapa Gado de Leite (CNPGL) |
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1. | | PILLAI, V. V.; SIQUEIRA, L. G. B.; DAS, M.; KEI, T. G.; TU, L. N.; HERREN, A. W.; PHINNEY, B. S.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V. Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. Biology Open, v. 8, n. 5, article bio037937, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite. |
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