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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
14/06/2017 |
Data da última atualização: |
03/07/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MIRANDA, V. de J.; PORTO, W. F.; FERNANDES, G. da R.; POGUE, R.; NOLASCO, D. O.; ARAUJO, A. C. G.; COTA, L. V.; FREITAS, C. G. de; DIAS, S. C.; FRANCO, O. L. |
Afiliação: |
LUCIANO VIANA COTA, CNPMS. |
Título: |
Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Scientific Reports, v. 7, n. 2483, p. 1-14, 2017. |
DOI: |
10.1038/s41598-017-02298-8 |
Idioma: |
Inglês Português |
Conteúdo: |
The hemibiotrophic fungus Colletotrichum graminicola may cause severe damage to maize, affecting normal development of the plant and decreasing grain yield. In this context, understanding plant defense pathways at the inoculation site and systemically in uninoculated tissues can help in the development of genetic engineering of resistance against this pathogen. Previous work has discussed the molecular basis of maize - C. graminicola interaction. However, many genes involved in defense have not yet been exploited for lack of annotation in public databases. Here, changes in global gene expression were studied in root, male and female inflorescences of maize under local and systemic fungal infection treatments, respectively. RNA-Seq with qPCR was used to indicate genes involved in plant defense. We found that systemic acquired resistance induction in female inflorescences mainly involves accumulation of salicylic acid (SA)-inducible defense genes (ZmNAC, ZmHSF, ZmWRKY, ZmbZIP and PR1) and potential genes involved in chromatin modification. Furthermore, transcripts involved in jasmonic acid (JA) and ethylene (ET) signaling pathways were also accumulated and may participate in plant immunity. Moreover, several genes were functionally re-annotated based on domain signature, indicating novel candidates to be tested in strategies involving gene knockout and overexpression in plants. |
Palavras-Chave: |
Maize; Resistência a doença. |
Thesagro: |
Antracnose; Colletotrichum Graminicola; Doença de planta; Gene; Milho. |
Categoria do assunto: |
-- H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161292/1/Comparative-transcriptomic.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160774/1/41598-2017-Article-2298.pdf
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Marc: |
LEADER 02366naa a2200325 a 4500 001 2071838 005 2017-07-03 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1038/s41598-017-02298-8$2DOI 100 1 $aMIRANDA, V. de J. 245 $aComparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize.$h[electronic resource] 260 $c2017 520 $aThe hemibiotrophic fungus Colletotrichum graminicola may cause severe damage to maize, affecting normal development of the plant and decreasing grain yield. In this context, understanding plant defense pathways at the inoculation site and systemically in uninoculated tissues can help in the development of genetic engineering of resistance against this pathogen. Previous work has discussed the molecular basis of maize - C. graminicola interaction. However, many genes involved in defense have not yet been exploited for lack of annotation in public databases. Here, changes in global gene expression were studied in root, male and female inflorescences of maize under local and systemic fungal infection treatments, respectively. RNA-Seq with qPCR was used to indicate genes involved in plant defense. We found that systemic acquired resistance induction in female inflorescences mainly involves accumulation of salicylic acid (SA)-inducible defense genes (ZmNAC, ZmHSF, ZmWRKY, ZmbZIP and PR1) and potential genes involved in chromatin modification. Furthermore, transcripts involved in jasmonic acid (JA) and ethylene (ET) signaling pathways were also accumulated and may participate in plant immunity. Moreover, several genes were functionally re-annotated based on domain signature, indicating novel candidates to be tested in strategies involving gene knockout and overexpression in plants. 650 $aAntracnose 650 $aColletotrichum Graminicola 650 $aDoença de planta 650 $aGene 650 $aMilho 653 $aMaize 653 $aResistência a doença 700 1 $aPORTO, W. F. 700 1 $aFERNANDES, G. da R. 700 1 $aPOGUE, R. 700 1 $aNOLASCO, D. O. 700 1 $aARAUJO, A. C. G. 700 1 $aCOTA, L. V. 700 1 $aFREITAS, C. G. de 700 1 $aDIAS, S. C. 700 1 $aFRANCO, O. L. 773 $tScientific Reports$gv. 7, n. 2483, p. 1-14, 2017.
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Embrapa Milho e Sorgo (CNPMS) |
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1. | | MIRANDA, V. de J.; PORTO, W. F.; FERNANDES, G. da R.; POGUE, R.; NOLASCO, D. O.; ARAUJO, A. C. G.; COTA, L. V.; FREITAS, C. G. de; DIAS, S. C.; FRANCO, O. L. Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize. Scientific Reports, v. 7, n. 2483, p. 1-14, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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