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| Registros recuperados : 12 | |
| 4. |  | DUPONT, P. B.; LIMA, C. L. R. de; PILLON, C. N.; ABREU, M. D.; COMPAGNONI, L.; STUMPF, L. Qualidade estrutural de um argissolo vermelho amarelo sob campo nativo e cultivo de milho em Turuçu, RS. In: SIMPÓSIO NACIONAL DE RECUPERAÇÃO DE ÁREAS DEGRADADAS, 8., 31 A 02 de nov. de 2010, Guarapari. anais... Curitiba: SOBRADE, 2010. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | STUMPF, L.; PAULETTO, E. A.; PINTO, L. F. S.; GARCIA, G. F.; AMBUS, J. V.; SILVA, T. S.; PINTO, M. A. B.; TUCHTENHAGEN, I. K. Condição física e desenvolvimento radicular de gramíneas em solo construído após mineração de carvão. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 9, p. 1078-1087, set. 2016. Título em inglês: Physical condition and root development of grasses in a constructed minesoil after coal mining.| Biblioteca(s): Embrapa Solos / UEP-Recife; Embrapa Unidades Centrais. |
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| 6. | | MARTINAZZO, R.; SILVEIRA, C. A. P.; BAMBERG, A. L.; STUMPF, L.; STOCKER, C. M.; MONTEIRO, A. B.; PEREIRA, I dos S.; RIBEIRO, P. L.; GIACOMINI, S. J. Incorporation of retorted oil shale in Brazilian agricultural soil: An assessment of impacts after successive applications. Journal of Cleaner Production, v. 245, 118652, Feb. 2020.| Biblioteca(s): Embrapa Clima Temperado. |
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| 7. | | MARTINAZZO, R.; MULLER, C. A.; TEIXEIRA, L. C.; STUMPF, L.; ANTUNES, w. r.; METZ, L. E. G.; VALGAS, R. A.; BAMBERG, A. L.; SILVEIRA, C. A. P. Leather-based fertilizers from Personal Protective Equipment (PPE) reverse logistics: Technical efficiency and environmental safety. Resources, Conservation & Recycling Advances, v. 18, 200153, Oct. 2023.| Biblioteca(s): Embrapa Clima Temperado; Embrapa Unidades Centrais. |
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| 8. | | BAMBERG, A. L.; MARTINAZZO, R.; SILVEIRA, C. A. P.; PILLON, C. N.; STUMPF, L.; BERGMANN, M.; VAN STRAATEN, P.; MARTINS, E. de S. Selected rock powders as sources of nutrients for soil fertilization and maize-wheat grain production in southern Brazil. The Journal of Agricultural Science, v. 161, n. 5, p. 654-668, Oct. 2023.| Biblioteca(s): Embrapa Clima Temperado; Embrapa Unidades Centrais. |
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| 9. | | PINTO, M. A. B.; PARFITT, J. M. B.; TIMM, L. C.; FARIA, L. C.; CONCENÇO, G.; STUMPF, L.; NÖRENBERG, B. G. Sprinkler irrigation in lowland rice: Crop yield and its components as afunction of water availability in different phenological phases. Field Crops Research, v. 248, 107714, 1 March 2020.| Biblioteca(s): Embrapa Clima Temperado. |
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| 10. | | PANZIERA, W.; LIMA, C. L. R. de; MOURA-BUENO, J. M.; PAULETTO, E. A.; SILVA, S. D. dos A. e; TIMM, L. C.; STUMPF, L. Spatial variability of soil physical attributes in sugarcane using different row spacings. Australian Journal of Crop Science, v. 14, n. 09, p. 1399-1404, 2020.| Biblioteca(s): Embrapa Clima Temperado. |
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| 11. | | PANZIERA, W.; LIMA, C. L. R. de; TIMM, L. C.; AQUINO, L. S.; BARROS, W. S.; STUMPF, L.; SILVA, S. D. dos A. e; MOURA-BUENO, J. M.; DUTRA JUNIOR, L. A.; PAULETTO, E. A. Investigating the relationships between soil and sugarcane attributes under different row spacing configurations and crop cycles using the state-space approach. Soil & Tillage Research, v. 217, 105270, 2022.| Biblioteca(s): Embrapa Clima Temperado. |
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| 12. | | SILVA, M. T. da; MARTINAZZO, R.; SILVA, S. D. dos A. e; BAMBERG, A. L.; STUMPF, L.; FERMINO, M. H.; KOHLER, T. W.; MATOSO, E. S.; VALGAS, R. A. Innovative substrates for sugarcane seedling production: Sewage sludges and rice husk ash in a waste-to-product strategy. Industrial Crops and Products, v. 157, 112812, 1 Dec. 2020.| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 12 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/11/2015 |
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Data da última atualização: |
31/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ROCHA, C. M. L.; VELLICCE, G. R.; GARCÍA, M. G.; PARDO, E. M.; RACEDO, J.; PERERA, M. F.; LUCÍA, A. de; GILLI, J.; BOGADO, N.; BONNECARRÈRE, V.; GERMAN, S.; MARCELINO, F.; LEDESMA, F.; REZNIKOV, S.; PLOPER, L. D.; WELIN, B.; CASTAGNARO, A. P. |
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Afiliação: |
CARLA MARIA LOURDES ROCHA, ITA - NOA; GABRIEL RICARDO VELLICCE, ITA - NOA; MARÍA GABRIELA GARCÍA, ITA - NOA; ESTEBAN MARIANO PARDO, ITA - NOA; JOSEFINA RACEDO, ITA - NOA; MARÍA FRANCISCA PERERA, ITA - NOA; ADRIAN DE LUCÍA, INTA; JAVIER GILLI, INTA; NOELIA BOGADO, IPTA; VICTORIA BONNECARRÈRE, INIA; SILVIA GERMAN, INIA; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; FERNANDO LEDESMA A ,, ITA - NOA; SEBASTIÁN REZNIKOV, ITA - NOA; LEONARDO DANIEL PLOPER, ITA - NOA; BJORN WELIN, ITA - NOA; ATILIO PEDRO CASTAGNARO, ITA- NOA. |
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Título: |
Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Electronic Journal of Biotechnology, v. 18, n. 6, p. 439-444, 2015. |
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ISSN: |
0717-3458 |
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DOI: |
10.1016/j.ejbt.2015.06.007 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. MenosBackground: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and pot... Mostrar Tudo |
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Palavras-Chave: |
Ferrugem asiática da soja. |
|
Thesagro: |
Doença de planta; Ferrugem; Fungo; Phakopsora pachyrhizi; Soja. |
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Thesaurus NAL: |
Microbial growth; Plant diseases and disorders; Soybean rust. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02845naa a2200445 a 4500
001 2028670
005 2017-07-31
008 2015 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $a10.1016/j.ejbt.2015.06.007$2DOI
100 1 $aROCHA, C. M. L.
245 $aUse of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi.$h[electronic resource]
260 $c2015
520 $aBackground: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.
650 $aMicrobial growth
650 $aPlant diseases and disorders
650 $aSoybean rust
650 $aDoença de planta
650 $aFerrugem
650 $aFungo
650 $aPhakopsora pachyrhizi
650 $aSoja
653 $aFerrugem asiática da soja
700 1 $aVELLICCE, G. R.
700 1 $aGARCÍA, M. G.
700 1 $aPARDO, E. M.
700 1 $aRACEDO, J.
700 1 $aPERERA, M. F.
700 1 $aLUCÍA, A. de
700 1 $aGILLI, J.
700 1 $aBOGADO, N.
700 1 $aBONNECARRÈRE, V.
700 1 $aGERMAN, S.
700 1 $aMARCELINO, F.
700 1 $aLEDESMA, F.
700 1 $aREZNIKOV, S.
700 1 $aPLOPER, L. D.
700 1 $aWELIN, B.
700 1 $aCASTAGNARO, A. P.
773 $tElectronic Journal of Biotechnology$gv. 18, n. 6, p. 439-444, 2015.
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