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| 2. |  | PINHEIRO, J. B. Manejo e danos de fitonematoides em hortaliças: cenoura, batata, gengibre, inhame, mandioquinha-salsa e tomate para processamento industrial. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 34., 2017, Vitória. Nematoides: manejo, desafios e soluções. Piracicaba: Sociedade Brasileira de Nematologia, 2017.| Biblioteca(s): Embrapa Hortaliças. |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/11/2021 |
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Data da última atualização: |
30/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SOUZA, S. de O.; OLIVEIRA, R. S. de; AONA, L. Y. S.; SOUZA, F. V. D.; SOARES, T. L.; ROSSI, M. L. |
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Afiliação: |
SANDRA DE OLIVEIRA SOUZA, UFRB; RAFAELLE SOUZA DE OLIVEIRA, UFRB; LIDYANNE YURIKO SALEME AONA, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; TALIANE LEILA SOARES, UEFS; MÔNICA LANZONI ROSSI, USP. |
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Título: |
Pollen morphology and viability of Tillandsia (Bromeliaceae) species by light microscopy and scanning electron microscopy. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Microscopy Reserarch Technique, p.1-19, 2020. |
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ISSN: |
1097-0029 |
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Idioma: |
Inglês |
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Conteúdo: |
Tillandsia is the bromeliad genus containing the largest number of species, with wide geographic dispersion and an important ecological role in the ecosystems. Investiga- tions of pollen morphology are important to support taxonomic and conservation studies as well as to choose promising species for cross-pollination to obtain hybrids. The objective of this study was to evaluate the morphology, production, and viability of pollen grains of 24 Tillandsia species that naturally occur in the state of Bahia, Bra- zil. Pollen grains were acetolized and analyzed by scanning electron microscopy. Thepollen were germinated in vitro in two culture media (BM and BKM) and collected at three floral development stages (pre-anthesis, anthesis and post-anthesis). Pollen via-bility also was analyzed by staining with Alexander's solution and acetocarmine in three floral development stages. Variations in the ornamentation and size of the pol-len were observed among the species studied, with the majority having medium size, while T. polystachia and T. juncea had large grains and the subgenera Diaphoranthema and Phytarrhiza had small grains. The pollen of the majority of species had bilateral symmetry, with a single irregularly shaped colpus, semitectate exine and reticulated-heterobrochate surface. The pollen presented high germination percentage, tube length and viability according to the histochemistry, except for T. recurvata, T. usneoides and T. loliacea. The BKM medium and collection of pollen during anthesis produced the best results for the Tillandsia species. The results can support studies ofthe taxonomy and conservation of these species, which have great environmental importance and high value for ornamental purposes. MenosTillandsia is the bromeliad genus containing the largest number of species, with wide geographic dispersion and an important ecological role in the ecosystems. Investiga- tions of pollen morphology are important to support taxonomic and conservation studies as well as to choose promising species for cross-pollination to obtain hybrids. The objective of this study was to evaluate the morphology, production, and viability of pollen grains of 24 Tillandsia species that naturally occur in the state of Bahia, Bra- zil. Pollen grains were acetolized and analyzed by scanning electron microscopy. Thepollen were germinated in vitro in two culture media (BM and BKM) and collected at three floral development stages (pre-anthesis, anthesis and post-anthesis). Pollen via-bility also was analyzed by staining with Alexander's solution and acetocarmine in three floral development stages. Variations in the ornamentation and size of the pol-len were observed among the species studied, with the majority having medium size, while T. polystachia and T. juncea had large grains and the subgenera Diaphoranthema and Phytarrhiza had small grains. The pollen of the majority of species had bilateral symmetry, with a single irregularly shaped colpus, semitectate exine and reticulated-heterobrochate surface. The pollen presented high germination percentage, tube length and viability according to the histochemistry, except for T. recurvata, T. usneoides and T. loliacea. The BKM medium and collection of po... Mostrar Tudo |
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Thesagro: |
Pólen. |
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Categoria do assunto: |
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Marc: |
LEADER 02359naa a2200205 a 4500
001 2136803
005 2021-11-30
008 2020 bl uuuu u00u1 u #d
022 $a1097-0029
100 1 $aSOUZA, S. de O.
245 $aPollen morphology and viability of Tillandsia (Bromeliaceae) species by light microscopy and scanning electron microscopy.$h[electronic resource]
260 $c2020
520 $aTillandsia is the bromeliad genus containing the largest number of species, with wide geographic dispersion and an important ecological role in the ecosystems. Investiga- tions of pollen morphology are important to support taxonomic and conservation studies as well as to choose promising species for cross-pollination to obtain hybrids. The objective of this study was to evaluate the morphology, production, and viability of pollen grains of 24 Tillandsia species that naturally occur in the state of Bahia, Bra- zil. Pollen grains were acetolized and analyzed by scanning electron microscopy. Thepollen were germinated in vitro in two culture media (BM and BKM) and collected at three floral development stages (pre-anthesis, anthesis and post-anthesis). Pollen via-bility also was analyzed by staining with Alexander's solution and acetocarmine in three floral development stages. Variations in the ornamentation and size of the pol-len were observed among the species studied, with the majority having medium size, while T. polystachia and T. juncea had large grains and the subgenera Diaphoranthema and Phytarrhiza had small grains. The pollen of the majority of species had bilateral symmetry, with a single irregularly shaped colpus, semitectate exine and reticulated-heterobrochate surface. The pollen presented high germination percentage, tube length and viability according to the histochemistry, except for T. recurvata, T. usneoides and T. loliacea. The BKM medium and collection of pollen during anthesis produced the best results for the Tillandsia species. The results can support studies ofthe taxonomy and conservation of these species, which have great environmental importance and high value for ornamental purposes.
650 $aPólen
700 1 $aOLIVEIRA, R. S. de
700 1 $aAONA, L. Y. S.
700 1 $aSOUZA, F. V. D.
700 1 $aSOARES, T. L.
700 1 $aROSSI, M. L.
773 $tMicroscopy Reserarch Technique, p.1-19, 2020.
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