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| 16. |  | BARBOSA, J. N. do N.; ALVES, R. M.; OLIVEIRA, C. C. de; FERNANDES, J. R. Q. Definição de níveis categóricos de descritores qualitativos, para utilização na caracterização foliar do cupuaçuzeiro. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | ALVES, F. V.; ALMEIDA, R. G. de; LAURA, V. A.; OLIVEIRA, C. C. de. Ambiência e bem estar animal de bovinos de corte em sistemas de integração lavoura-pecuária-floresta (iLPF). In: CONGRESO COLOMBIANO, 2.; SEMINARIO INTERNACIONAL DE SILVOPASTOREO, 1., 2012, Medellín. Reforestacion con ganaderia, propuesta innovadora y sostenible. Medellín: Universidad Nacional de Colombia, 2012. 10 p.| Biblioteca(s): Embrapa Gado de Corte. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
29/05/2019 |
|
Data da última atualização: |
02/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CAMARGO, A. C. de; BIASOTO, A. C. T.; SCHWEMBER, A. R.; GRANADO, D.; RASERA, G. B.; FRANCHIN, M.; ROSALEN, P. L.; ALENCAR, S. M.; SHAHIDI, F. |
|
Afiliação: |
Adriano Costa de Camargo; ALINE TELLES BIASOTO MARQUES, CPATSA; Andrés R. Schwember; Daniel Granato; Gabriela Boscariol Rasera; Marcelo Franchin; Pedro L. Rosalen; Severino Matias Alencar; Fereidoon Shahidi. |
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Título: |
Should we ban total phenolics and antioxidant screening methods? The link between antioxidant potential and activation of NF-KB using phenolic compounds from grape by-products. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Food Chemistry, v. 290, p. 229-238, 2019. |
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DOI: |
10.1016/j.foodchem.2019.03.145 |
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Idioma: |
Inglês |
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Conteúdo: |
Free radical imbalance is associated with several chronic diseases. However, recent controversies have put in check the validity of colorimetric methods to screen the functionality of polyphenols. Therefore, in this study two antioxidant methods, based on chemical reactions, were tested for their ability in anticipating the reduction of the activation of NF-κB using LPS-activated RAW 264.7 macrophages, selected as a biological model. Grape processing by-products from winemaking showed higher total phenolic content (TPC), antioxidant capacity towards peroxyl radical (31.1%) as well as reducing power (39.5%) than those of grape juice by-products. The same trend was observed when these samples were tested against LPS-activated RAW 264.7 macrophages by reducing the activation NF-κB. Feedstocks containing higher TPC and corresponding ORAC and FRAP results translated to higher reduction in the activation of NF-κB (36.5%). Therefore, this contribution demonstrates that colorimetric methods are still important screening tools owing their simplicity and widespread application. |
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Palavras-Chave: |
Compostos fenólicos; Fenólicos bioativos; Redução de poder antioxidante férrico. |
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Thesagro: |
Subproduto; Uva. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02041naa a2200289 a 4500
001 2109420
005 2019-12-02
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.foodchem.2019.03.145$2DOI
100 1 $aCAMARGO, A. C. de
245 $aShould we ban total phenolics and antioxidant screening methods? The link between antioxidant potential and activation of NF-KB using phenolic compounds from grape by-products.$h[electronic resource]
260 $c2019
520 $aFree radical imbalance is associated with several chronic diseases. However, recent controversies have put in check the validity of colorimetric methods to screen the functionality of polyphenols. Therefore, in this study two antioxidant methods, based on chemical reactions, were tested for their ability in anticipating the reduction of the activation of NF-κB using LPS-activated RAW 264.7 macrophages, selected as a biological model. Grape processing by-products from winemaking showed higher total phenolic content (TPC), antioxidant capacity towards peroxyl radical (31.1%) as well as reducing power (39.5%) than those of grape juice by-products. The same trend was observed when these samples were tested against LPS-activated RAW 264.7 macrophages by reducing the activation NF-κB. Feedstocks containing higher TPC and corresponding ORAC and FRAP results translated to higher reduction in the activation of NF-κB (36.5%). Therefore, this contribution demonstrates that colorimetric methods are still important screening tools owing their simplicity and widespread application.
650 $aSubproduto
650 $aUva
653 $aCompostos fenólicos
653 $aFenólicos bioativos
653 $aRedução de poder antioxidante férrico
700 1 $aBIASOTO, A. C. T.
700 1 $aSCHWEMBER, A. R.
700 1 $aGRANADO, D.
700 1 $aRASERA, G. B.
700 1 $aFRANCHIN, M.
700 1 $aROSALEN, P. L.
700 1 $aALENCAR, S. M.
700 1 $aSHAHIDI, F.
773 $tFood Chemistry$gv. 290, p. 229-238, 2019.
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