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| Registros recuperados : 3 | |
| 2. |  | HASEGAWA, M.; SUGIURA, S.; ITO, M. T.; YAMAKI, A.; HAMAGUCHI, K.; KISHIMOTO, T.; OKOCHI, I. Community structures of soil animals on Chichijima island in Ogasawara islands in relation to the survival of land snails. In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.| Biblioteca(s): Embrapa Florestas. |
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| 3. | | HASEGAWA, M.; SUGIURA, S.; ITO, M. T.; YAMAKI, A.; HAMAGUCHI, K.; KISHIMOTO, T.; OKOCHI, I. Community structures of soil animals and survival of land snails on an island of the Ogasawara Archipelago. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 8, p. 896-903, ago. 2009. Título em português: Estruturas de comunidades de animais de solo e sobrevivência dos caracóis terrestres numa ilha do Arquipélago Ogasawara.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Unidades Centrais. |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
02/12/2010 |
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Data da última atualização: |
27/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
HERAI, R. H.; YAMAGISHI, M. E. B. |
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Afiliação: |
ROBERTO H. HERAI, IB/UNICAMP; MICHEL E. B. YAMAGISHI, CNPTIA. |
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Título: |
A bioinformatics approach to detect interchromosomal trans-splicing in bovine full length cDNA databanks. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: PLANT & ANIMAL GENOME CONFERENCE, 18., 2010, San Diego. Abstracts... [S.l.: s.n.], 2010. |
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Páginas: |
Não paginado. |
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Idioma: |
Inglês |
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Notas: |
P869. PAG-XVIII. |
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Conteúdo: |
Trans-splicing is an unusual form of RNA processing mechanism where distinct pre-mRNA sequences contribute to the formation of a single mRNA. It is common in nematodes and kinetoplastids, but it is rare in superior organisms. Nevertheless, some recent studies show that trans-splicing, although rare, may be more widespread than believed (Gingeras). Consequently, its identification in large databanks is very important for posteriori experimental confirmation. Recently, we have developed a Bioinformatic methodology that successfully found 16 inter-chromosomal trans-splicing candidate sequences in a large human full length cDNA (FlcDNA) databank (Herai & Yamagishi, in press). Unfortunately, our methodology was supposed to be applied on organisms with high quality reference genome, otherwise the number of false positive candidate sequences turned the analysis prohibitive. The problem is that many important model organisms have only draft assemblies. In order to overcome this problem, we extended our bioinformatics methodology applying additional filtering criteria and ad hoc algorithms necessary to deal with those cases. Using 83,048 bovine FlcDNA transcripts (NCBI, MGC, BGD) and Bos taurus UMD 3.0 genome assembly, our extended methodology successfully found 12 hybrid mRNAs which may be the first instances of bovine inter-chromosomal trans-splicing sequences, since the single reported bovine trans-splicing evidence was an intra-chromosomal instance (Roux et al.). Furthermore, just like in the human case, the bovine candidate sequence gene loci had many inverted repeat sequences which may support the conjecture that those sequences may be part of a non-spliceosome mediated trans-splicing mechanism (Di Segni et al.). MenosTrans-splicing is an unusual form of RNA processing mechanism where distinct pre-mRNA sequences contribute to the formation of a single mRNA. It is common in nematodes and kinetoplastids, but it is rare in superior organisms. Nevertheless, some recent studies show that trans-splicing, although rare, may be more widespread than believed (Gingeras). Consequently, its identification in large databanks is very important for posteriori experimental confirmation. Recently, we have developed a Bioinformatic methodology that successfully found 16 inter-chromosomal trans-splicing candidate sequences in a large human full length cDNA (FlcDNA) databank (Herai & Yamagishi, in press). Unfortunately, our methodology was supposed to be applied on organisms with high quality reference genome, otherwise the number of false positive candidate sequences turned the analysis prohibitive. The problem is that many important model organisms have only draft assemblies. In order to overcome this problem, we extended our bioinformatics methodology applying additional filtering criteria and ad hoc algorithms necessary to deal with those cases. Using 83,048 bovine FlcDNA transcripts (NCBI, MGC, BGD) and Bos taurus UMD 3.0 genome assembly, our extended methodology successfully found 12 hybrid mRNAs which may be the first instances of bovine inter-chromosomal trans-splicing sequences, since the single reported bovine trans-splicing evidence was an intra-chromosomal instance (Roux et al.). Furthermore, jus... Mostrar Tudo |
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Palavras-Chave: |
Algoritmo; Bioinformática. |
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Thesaurus NAL: |
Algorithms; Bioinformatics; RNA splicing. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/868482/1/869.pdf
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Marc: |
LEADER 02412nam a2200205 a 4500
001 1868482
005 2020-01-27
008 2010 bl uuuu u00u1 u #d
100 1 $aHERAI, R. H.
245 $aA bioinformatics approach to detect interchromosomal trans-splicing in bovine full length cDNA databanks.$h[electronic resource]
260 $aIn: PLANT & ANIMAL GENOME CONFERENCE, 18., 2010, San Diego. Abstracts... [S.l.: s.n.], 2010.$c2010
300 $aNão paginado.
500 $aP869. PAG-XVIII.
520 $aTrans-splicing is an unusual form of RNA processing mechanism where distinct pre-mRNA sequences contribute to the formation of a single mRNA. It is common in nematodes and kinetoplastids, but it is rare in superior organisms. Nevertheless, some recent studies show that trans-splicing, although rare, may be more widespread than believed (Gingeras). Consequently, its identification in large databanks is very important for posteriori experimental confirmation. Recently, we have developed a Bioinformatic methodology that successfully found 16 inter-chromosomal trans-splicing candidate sequences in a large human full length cDNA (FlcDNA) databank (Herai & Yamagishi, in press). Unfortunately, our methodology was supposed to be applied on organisms with high quality reference genome, otherwise the number of false positive candidate sequences turned the analysis prohibitive. The problem is that many important model organisms have only draft assemblies. In order to overcome this problem, we extended our bioinformatics methodology applying additional filtering criteria and ad hoc algorithms necessary to deal with those cases. Using 83,048 bovine FlcDNA transcripts (NCBI, MGC, BGD) and Bos taurus UMD 3.0 genome assembly, our extended methodology successfully found 12 hybrid mRNAs which may be the first instances of bovine inter-chromosomal trans-splicing sequences, since the single reported bovine trans-splicing evidence was an intra-chromosomal instance (Roux et al.). Furthermore, just like in the human case, the bovine candidate sequence gene loci had many inverted repeat sequences which may support the conjecture that those sequences may be part of a non-spliceosome mediated trans-splicing mechanism (Di Segni et al.).
650 $aAlgorithms
650 $aBioinformatics
650 $aRNA splicing
653 $aAlgoritmo
653 $aBioinformática
700 1 $aYAMAGISHI, M. E. B.
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Embrapa Agricultura Digital (CNPTIA) |
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