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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/11/2004 |
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Data da última atualização: |
23/11/2015 |
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Autoria: |
CARVALHO, C. H. S.; ZEHR, U. B.; GUNARATNA, N.; ANDERSON, J.; KONONOWICZ, H. H.; HODGES, T. K.; AXTELL, J. D. |
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Afiliação: |
CARLOS HENRIQUE S DE CARVALHO, Embrapa Milho e Sorgo; Usha B. Zehr, Purdue University; Nilupa Gunaratna, Purdue University; Joseph Anderson, Purdue University; Halina H. Kononowicz, Purdue University; Thomas K. Hodges, Purdue University; John D. Axtell, Purdue University. |
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Título: |
Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 27, n. 2, p. 259-269, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection. MenosThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculu... Mostrar Tudo |
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Thesagro: |
Sorgo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32593/1/Agrobacterium-mediated.pdf
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Marc: |
LEADER 02225naa a2200205 a 4500
001 1488374
005 2015-11-23
008 2004 bl uuuu u00u1 u #d
100 1 $aCARVALHO, C. H. S.
245 $aAgrobacterium-mediated transformation of sorghum$bfactors that affect transformation efficiency.$h[electronic resource]
260 $c2004
520 $aThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection.
650 $aSorgo
700 1 $aZEHR, U. B.
700 1 $aGUNARATNA, N.
700 1 $aANDERSON, J.
700 1 $aKONONOWICZ, H. H.
700 1 $aHODGES, T. K.
700 1 $aAXTELL, J. D.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 27, n. 2, p. 259-269, 2004.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
04/12/1996 |
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Data da última atualização: |
07/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, P. A. C.; NUENBERG, S.; PIMENTEL NETO, M.; OLIVEIRA, G. P. de; REZENDE, H. E. B. de; ARAUJO, J. L. de B.; MELLO, R. P. de. |
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Afiliação: |
PLINIO ANTONIO COSTA GOMES, GEPA-RJ; SILVINO NUERNBERG, IPEACS-MA; MANOEL PIMENTEL NETO, IPEACS-MA; GILSON PEREIRA DE OLIVEIRA, CPPSE; HUGO EDISON BARBOZA DE REZENDE, UFRRJ; JOSÉ LUIZ DE BARROS ARAUJO, UFRRJ; RUBENS PINTO DE MELLO, UFRRJ. |
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Título: |
Infecção experimental de Lymnaea columella Say, 1817, com Fasciola hepatica Linnaeus, 1758, de ocorrência no estado do Rio de Janeiro. |
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Ano de publicação: |
1974 |
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Fonte/Imprenta: |
Arquivo da Universidade Federal Rural, Rio de Janeiro, v. 4, n. 1, p. 35-38, 1974. |
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Idioma: |
Português |
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Conteúdo: |
Os autores observaram o comportamento dos moluscos submetidos a infecção com quantidades variáveis de miracidios de fasciola hepática. Estes miracidios foram obtidos pela incubação de ovos de Fasciola hepática recolhidos diretamente de visicula biliar de bovinos infectados, nascidos e criados no Estado do Rio de Janeiro, provenientes de matadouro. A eclosão ocorreu entre 9 e 12 dias , a temperatura de 27 a 29 graus e os miracidios sobreviverem, aproximadamente, 4 horas. |
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Palavras-Chave: |
Bovine; Lymnaea colunella; Rio de Janeiro. |
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Thesagro: |
Bovino; Fascíola. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/121774/1/digitalizar0009.pdf
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Marc: |
LEADER 01290naa a2200253 a 4500
001 1043342
005 2023-06-07
008 1974 bl uuuu u00u1 u #d
100 1 $aGOMES, P. A. C.
245 $aInfecção experimental de Lymnaea columella Say, 1817, com Fasciola hepatica Linnaeus, 1758, de ocorrência no estado do Rio de Janeiro.$h[electronic resource]
260 $c1974
520 $aOs autores observaram o comportamento dos moluscos submetidos a infecção com quantidades variáveis de miracidios de fasciola hepática. Estes miracidios foram obtidos pela incubação de ovos de Fasciola hepática recolhidos diretamente de visicula biliar de bovinos infectados, nascidos e criados no Estado do Rio de Janeiro, provenientes de matadouro. A eclosão ocorreu entre 9 e 12 dias , a temperatura de 27 a 29 graus e os miracidios sobreviverem, aproximadamente, 4 horas.
650 $aBovino
650 $aFascíola
653 $aBovine
653 $aLymnaea colunella
653 $aRio de Janeiro
700 1 $aNUENBERG, S.
700 1 $aPIMENTEL NETO, M.
700 1 $aOLIVEIRA, G. P. de
700 1 $aREZENDE, H. E. B. de
700 1 $aARAUJO, J. L. de B.
700 1 $aMELLO, R. P. de
773 $tArquivo da Universidade Federal Rural, Rio de Janeiro$gv. 4, n. 1, p. 35-38, 1974.
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Embrapa Pecuária Sudeste (CPPSE) |
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