Data/hora: 17/06/2024 11:48:06

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 16/09/1993
Data da última atualização: | 16/09/1993
Autoria: | YOKOMI, R. K.; GARNSEY, S. M.; PERMAR, T. A.; LEE, R. F.; YOUTSEY, C. O.
Afiliação: | USDA, ARS, 2120 Camden Road, Orlando, FL 32803, USA.
Título: | Natural spread of severe citrus tristeza virus isolates in citrus preinfected with mild CTV isolates.
Ano de publicação: | 1991
Fonte/Imprenta: | Riverside, California: International Organization of Citrus Virologists, 1991.
Páginas: | p.86-92
Idioma: | Inglês
Notas: | Conference of the International Organization of Citrus virologists, 11., 1991, Riverside, California, Proceedings...
Conteúdo: | Natural spread of severe citrus tristeza virus (CTV) isolates was monitored in a 2-ha, 306-tree plot near Dundee, Florida. This plot, planted in spring 1986, consisted of Hamlin orange and Redblush grapefruit trees on sour orange rootstock preinfected with fourteen different CTV mild isolates. The trees were surveyed for symptoms of CTV decline in November 1988, March 1989, and October 1989, and assayed by enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody, CTV-MCA13, which reacts specifically to severe decline-inducing isolates of CTV, and a broad-spectrum monoclonal antibody, 3DF1, were used in double sandwich indirect assay. After 3 yr of natural challenge, 3DF1-based ELISA indicated that 10 of 18 (56%) Hamlin and four of 19 (21%) Redblush grapefruit control trees became infected with CTV; seven of these Hamlin and all Redblush trees naturally infected with CTV also reacted with CTV-MCA13, indicating that they were infected with a severe isolate. Infection levels of CTV severe isolates in preinfected Hamlin treatments ranged from 0 to 50% based on CTV-MCA13 ELISA, with two treatments free of severe isolates. In Redblush grapefruit, infection levels of CTV severe isolates ranged from 0 to 22% but 11 CTV mild isolate treatments remained free from severe CTV infection. Decline symptoms were observed in 34 Hamlin and six grapefruit trees and supported the CTV-MCA13 ELISA results. Although substantial differences occurred between Hamlin treatments, results were not statistically different due to the ...
Palavras-Chave: | Aphid vectors; Mild strain cross protection; Mnoclonal antibody; Quick decline; tristeza control.
Thesagro: | Elisa.
Thesaurus Nal: | epidemiology.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13278-1) - ADC - PL - -- - 634.3040601 - C748p - 1992000183 - DIS