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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/01/1996 |
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Data da última atualização: |
05/06/2023 |
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Autoria: |
YOUNIS, A. I.; ZUELKE, K. A.; HARPER, K. M.; OLIVEIRA, M. A. L.; BRACKETT, B. G. |
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Título: |
In vitro fertilization of goat oocytes. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Biology of Reproduction, v. 44, n. 46, p. 1177-1182, Jun. 1991. |
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DOI: |
10.1095/biolreprod44.6.1177 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Experiments were carried out to achieve fertilization (IVF) and initial embryonic development of goat oocytes in vitro. Oocyte/cumulus complexes were recovered from large follicles (greater than 7 mm) of hormonally treated doses and from 1-6-mm follicles of ovaries from hormonally superstimulated and nontreated goats. Three different sperm treatment/IVF media were used: defined medium (Brackett and Oliphant, Biol Reprod 1975; 12:260-274) with modifications (mDM); TALP (Bavister and Yanagimachi, Biol Reprod 1977; 16:228-237), as modified by Parrish et al. (Theriogenology 1986; 25:591-600), i.e. modified TALP (mTALP); and HEPES-buffered M199 with modifications (mH-M199). Immature oocytes (from 1-6 mm, small antral follicles) were cultured for in vitro maturation (IVM) in M199 buffered with bicarbonate and with modifications including supplementation with 20% (v/v) goat serum (mB-M199) with either (a) 100 micrograms LH/ml, (b) 5 micrograms FSH/ml, or (c) no added gonadotropin control. Insemination of (in vivo or in vitro) matured oocytes was performed with swim-up separated and heparin-treated freshly ejaculated sperm; additionally, caffeine was included in the mDM treatment. Use of mDM yielded better results than mTALP or mH-M199 (p less than .05). Results with oocytes after IVM were significantly better than those obtained with oocytes matured in vivo (68.4% vs. 45.5%, p less than 0.05). Presence of LH or FSH during oocyte maturation improved both the IVM and IVF results over those of the control (p less than 0.05). The highest proportion of fertilized oocytes (fertilization rate) was achieved by combining the use of mDM for sperm and IVF with IVM in the presence of LH. MenosAbstract: Experiments were carried out to achieve fertilization (IVF) and initial embryonic development of goat oocytes in vitro. Oocyte/cumulus complexes were recovered from large follicles (greater than 7 mm) of hormonally treated doses and from 1-6-mm follicles of ovaries from hormonally superstimulated and nontreated goats. Three different sperm treatment/IVF media were used: defined medium (Brackett and Oliphant, Biol Reprod 1975; 12:260-274) with modifications (mDM); TALP (Bavister and Yanagimachi, Biol Reprod 1977; 16:228-237), as modified by Parrish et al. (Theriogenology 1986; 25:591-600), i.e. modified TALP (mTALP); and HEPES-buffered M199 with modifications (mH-M199). Immature oocytes (from 1-6 mm, small antral follicles) were cultured for in vitro maturation (IVM) in M199 buffered with bicarbonate and with modifications including supplementation with 20% (v/v) goat serum (mB-M199) with either (a) 100 micrograms LH/ml, (b) 5 micrograms FSH/ml, or (c) no added gonadotropin control. Insemination of (in vivo or in vitro) matured oocytes was performed with swim-up separated and heparin-treated freshly ejaculated sperm; additionally, caffeine was included in the mDM treatment. Use of mDM yielded better results than mTALP or mH-M199 (p less than .05). Results with oocytes after IVM were significantly better than those obtained with oocytes matured in vivo (68.4% vs. 45.5%, p less than 0.05). Presence of LH or FSH during oocyte maturation improved both the IVM and IVF re... Mostrar Tudo |
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Palavras-Chave: |
Cell separation; Cytology; Drug effects; Fertilizacao in vitro; FIV. |
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Thesagro: |
Caprino. |
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Thesaurus Nal: |
Culture media; Embryo transfer; Endocrinology; Females; Goats; Hormones; Oocytes; Pharmacology; Pregnancy; Reproduction. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02671naa a2200373 a 4500
001 1522474
005 2023-06-05
008 1991 bl uuuu u00u1 u #d
024 7 $a10.1095/biolreprod44.6.1177$2DOI
100 1 $aYOUNIS, A. I.
245 $aIn vitro fertilization of goat oocytes.$h[electronic resource]
260 $c1991
520 $aAbstract: Experiments were carried out to achieve fertilization (IVF) and initial embryonic development of goat oocytes in vitro. Oocyte/cumulus complexes were recovered from large follicles (greater than 7 mm) of hormonally treated doses and from 1-6-mm follicles of ovaries from hormonally superstimulated and nontreated goats. Three different sperm treatment/IVF media were used: defined medium (Brackett and Oliphant, Biol Reprod 1975; 12:260-274) with modifications (mDM); TALP (Bavister and Yanagimachi, Biol Reprod 1977; 16:228-237), as modified by Parrish et al. (Theriogenology 1986; 25:591-600), i.e. modified TALP (mTALP); and HEPES-buffered M199 with modifications (mH-M199). Immature oocytes (from 1-6 mm, small antral follicles) were cultured for in vitro maturation (IVM) in M199 buffered with bicarbonate and with modifications including supplementation with 20% (v/v) goat serum (mB-M199) with either (a) 100 micrograms LH/ml, (b) 5 micrograms FSH/ml, or (c) no added gonadotropin control. Insemination of (in vivo or in vitro) matured oocytes was performed with swim-up separated and heparin-treated freshly ejaculated sperm; additionally, caffeine was included in the mDM treatment. Use of mDM yielded better results than mTALP or mH-M199 (p less than .05). Results with oocytes after IVM were significantly better than those obtained with oocytes matured in vivo (68.4% vs. 45.5%, p less than 0.05). Presence of LH or FSH during oocyte maturation improved both the IVM and IVF results over those of the control (p less than 0.05). The highest proportion of fertilized oocytes (fertilization rate) was achieved by combining the use of mDM for sperm and IVF with IVM in the presence of LH.
650 $aCulture media
650 $aEmbryo transfer
650 $aEndocrinology
650 $aFemales
650 $aGoats
650 $aHormones
650 $aOocytes
650 $aPharmacology
650 $aPregnancy
650 $aReproduction
650 $aCaprino
653 $aCell separation
653 $aCytology
653 $aDrug effects
653 $aFertilizacao in vitro
653 $aFIV
700 1 $aZUELKE, K. A.
700 1 $aHARPER, K. M.
700 1 $aOLIVEIRA, M. A. L.
700 1 $aBRACKETT, B. G.
773 $tBiology of Reproduction$gv. 44, n. 46, p. 1177-1182, Jun. 1991.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | YOUNIS, A. I.; KESKINTEPE, L.; SIMPLICIO, A. A.; GOULD, K.; BRACKETT, B. G. Effects of EGTA and cytochalasin-B during freezing and vitrification of immature and mature bovine and rhesus monkey (M. mulatta) oocytes. Theriogenology, v. 47, n. 1, p. 361, 1997. Proceedings Annual Conference of the International Embryo Transfer Society, Nice Acropolis, Nice, France, Jan., 1997.| Tipo: Resumo em Anais de Congresso |
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