Data/hora: 21/06/2024 18:58:30

Biblioteca(s): | Embrapa Pecuária Sudeste.
Data corrente: | 26/11/2009
Data da última atualização: | 15/03/2023
Autoria: | MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M.
Afiliação: | SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, USP; FELIPE PERECIN, USP; NAIARA ZOCCAL SARAIVA, UNESP; TATIANE ALMEIDA DRUMMOND TETZNER, UNESP; WALT YAMAZAKI, UNESP; CLÁUDIA LIMA VERDE LEA, USP; FLÁVIO VIEIRA MEIRELLES, USP; JOAQUIM MANSANO GARCIA, UNESP.
Título: | Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Ano de publicação: | 2009
Fonte/Imprenta: | Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009.
DOI: | https://doi.org/10.1016/j.anireprosci.2009.02.020
Idioma: | Inglês
Conteúdo: | Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT. Developmental arrest up to eight-cell stage was greater in SCDN and DCSN than in S-PT and D-PT. In conclusion, karyoplast exchange between parthenogenetic zygotes activated with strontium and 6-DMAP can lead to nuclear?cytoplasmic incompatibilities and affect embryonic development to the eight-cell and blastocyst stages.
Palavras-Chave: | Artificial activation; Bovine; Embryonic development; Pronuclear transfer.
Thesaurus Nal: | beef cattle.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (18842-1) - UPC - AP - DD - PROCI-2009.00015 - MEO - 2009.00015 - DIS