| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
09/12/2019 |
|
Data da última atualização: |
09/12/2019 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
SILVA, N. M. L.; IANELLA, P.; YAMAGISHI, M. E. B.; PAIVA, S. R.; ROCHA, J. L.; TEIXEIRA, A. K.; FARIAS, F. G.; GUERRELHAS, A. C.; CAETANO, A. R. |
|
Afiliação: |
NAYELLE MEYRE LISBOA SILVA, UNB; PATRICIA IANELLA, Cenargen; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; SAMUEL REZENDE PAIVA, Cenargen; JOÃO LUIS ROCHA, GENEARCH AQUACULTURA; ANA KARINA TEIXEIRA, GENEARCH AQUACULTURA; FLÁVIO GALVÃO FARIAS, GENEARCH AQUACULTURA; ANA CAROLINA GUERRELHAS, GENEARCH AQUACULTURA; ALEXANDRE RODRIGUES CAETANO, Cenargen. |
|
Título: |
Development and validation of a low-density SNP panel for parentage control and evaluation of genetic diversity of pacific white shrimp (Litopenaeus vannamei). |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
In: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Plant and animal genome abstracts. Livingston, NJ: Scherago, 2019. |
|
Idioma: |
Inglês |
|
Notas: |
PE0274. |
|
Thesaurus Nal: |
Litopenaeus vannamei. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00807nam a2200217 a 4500
001 2116355
005 2019-12-09
008 2019 bl uuuu u00u1 u #d
100 1 $aSILVA, N. M. L.
245 $aDevelopment and validation of a low-density SNP panel for parentage control and evaluation of genetic diversity of pacific white shrimp (Litopenaeus vannamei).$h[electronic resource]
260 $aIn: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Plant and animal genome abstracts. Livingston, NJ: Scherago$c2019
500 $aPE0274.
650 $aLitopenaeus vannamei
700 1 $aIANELLA, P.
700 1 $aYAMAGISHI, M. E. B.
700 1 $aPAIVA, S. R.
700 1 $aROCHA, J. L.
700 1 $aTEIXEIRA, A. K.
700 1 $aFARIAS, F. G.
700 1 $aGUERRELHAS, A. C.
700 1 $aCAETANO, A. R.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
22/04/2008 |
|
Data da última atualização: |
16/03/2015 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Internacional - A |
|
Autoria: |
ROUWS, L. F. M.; SIMÕES ARAÚJO, J. L.; HEMERLY, A. S.; BALDANI, J. I. |
|
Afiliação: |
Luc F. M. Rouws, UFRRJ; Jean Luis Simões Araújo, Embrapa Agrobiologia; Adriana S. Hemerly, Instituto de Pesquisas do Jardim Botânico do RJ; José Ivo Baladani, Embrapa Agrobiologia. |
|
Título: |
Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
Archives of Microbiology, New York, v. 189, n. 4, p. 397-405, apr. 2008. |
|
Idioma: |
Inglês |
|
Notas: |
Parceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro. |
|
Conteúdo: |
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool. |
|
Palavras-Chave: |
Bactéria endofítica. |
|
Thesaurus NAL: |
Gluconacetobacter diazotrophicus. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01936naa a2200193 a 4500
001 1626792
005 2015-03-16
008 2008 bl uuuu u00u1 u #d
100 1 $aROUWS, L. F. M.
245 $aValidation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant.
260 $c2008
500 $aParceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro.
520 $aGluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?<KAN-2>Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool.
650 $aGluconacetobacter diazotrophicus
653 $aBactéria endofítica
700 1 $aSIMÕES ARAÚJO, J. L.
700 1 $aHEMERLY, A. S.
700 1 $aBALDANI, J. I.
773 $tArchives of Microbiology, New York$gv. 189, n. 4, p. 397-405, apr. 2008.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
